Biomedical subjects

Eva Mattsson

Publications and source records attributed to Eva Mattsson.

11 recordsLinked to original sources

PubMed2006

Six-minute walk test in adults with cerebral palsy. A study of reliability.

OBJECTIVE: To determine the reliability of a six-minute walk test in adults with cerebral palsy. DESIGN: Four repeated walk tests during two weeks. SETTING: The tests were conducted in a 40-m-long corridor at a rehabilitation centre for outpatients. SUBJECTS: Twenty-five adults (9 women and 16 men) with CP. Mean age 36 (26-58) years. Twelve subjects walked with walking aids and 13 without. INTERVENTIONS: No intervention. MEASURES: The subjects were instructed to walk as far as possible for 6 min. The distance walked, heart rate and perceived exertion were assessed. RESULTS: Mean distances walked by the subjects in the four tests were 316, 336, 341 and 345 m. The repeatability between test 1 and the other tests was 66 m, and when the first walk test was excluded 40 m. The coefficient of variation (CV) between test 1 and the other tests varied between 7.1 and 8.7%, and when the first walk test was excluded between 3.7 and 4.5%. Intraclass correlation (ICC 1.1) was 0.97 in the four tests, and when the first walk test was excluded 0.99. Walking distance was longer (P<0.01) and varied less in subjects walking without walking aids (n = 13) compared with subjects using walking aids (n = 12). CONCLUSIONS: Six-minute walk test showed high reliability, with a repeatability of 40 m. One practice test is recommended, as the walking distance in the first test was significantly shorter (P<0.01) than the other three tests.

Adult↗

PubMed2005

Physical activity and self-perception in school children assessed with the Children and Youth--Physical Self-Perception Profile.

AIM: This study validated the Swedish translation of the Children and Youth--Physical Self-Perception Profile (CY-PSPP) scale and examined the relation between physical self-perception and daily physical activity as well as the relationship between physical self-perception and body mass index (BMI) among Swedish school children. METHODS: Forty-eight children aged 11-12 years completed the CY-PSPP twice with 2 weeks in between. Test-retest reliability, concurrent and content validity were calculated. Five hundred and one children, aged 10-14 years, were measured for height and weight and perceived physical self-perception. Activity levels were analyzed using pedometers for 4 consecutive days. RESULTS: Good validity concerning concurrent and content validity was found. Test-retest reliability over a 2-week period was acceptable. In boys a fair and in girls a poor correlation between the sub-domains of the CY-PSPP and physical activity were found and a fair negative correlation between the sub-domains and BMI except for Physical Strength. CONCLUSION: The CY-PSPP distinguishes between children with low and high physical self-perception. The information is of importance when designing physical activity programs reachable for children with low physical self-esteem. According to the findings it is important to form physical activity programs that support and develop Sport Competence, Physical Condition and sense of Body Attractiveness among children.

Adolescent↗

PubMed2004

Low antibody levels against cell wall-attached proteins of Streptococcus pyogenes predispose for severe invasive disease.

Acute-phase serum samples from 70 patients with group A streptococcal (GAS) invasive disease were analyzed for IgG antibodies against 6 recently characterized GAS virulence factors (SclA, SclB, GRAB, MtsA, EndoS, and IdeS) and SpeB. Antibody levels against the cell wall-attached GAS antigens SclA, SclB, and GRAB were significantly lower in patients with severe invasive disease (streptococcal toxic shock syndrome [STSS] and/or necrotizing fasciitis [NF]; n=35), compared with levels in patients with nonsevere GAS bacteremia (n=35). Among patients with severe invasive disease, significantly lower antibody levels against GRAB were found in patients with STSS (n=10) than in patients with NF (n=17). Antibody levels against SpeB in patients with severe bacteremia were similar to those in patients with nonsevere bacteremia, and levels in patients with STSS were similar to those in patients with NF. The data indicate that immunity to cell wall-attached proteins may play a role in the protection against severe invasive disease and that antibodies against GRAB may be of importance in the pathogenesis of STSS.

Antibodies, Bacterial↗

PubMed2004

Highly purified lipoteichoic acid from Staphylococcus aureus induces procoagulant activity and tissue factor expression in human monocytes but is a weak inducer in whole blood: comparison with peptidoglycan.

Lipoteichoic acid from Staphylococcus aureus was a potent inducer of procoagulant activity in isolated mononuclear cells but not in whole blood. In contrast, staphylococcal peptidoglycan showed equal levels of potency in isolated mononuclear cells and whole blood, suggesting that peptidoglycan is an important inducer of procoagulant activity in severe sepsis involving gram-positive bacteria.

Blood↗

PubMed2003

Long-term effects of exercise training on physical activity level and quality of life in elderly coronary patients--a three- to six-year follow-up.

BACKGROUND AND PURPOSE: Physical activity and exercise as part of cardiac rehabilitation after an acute coronary event improves exercise capacity and quality of life in most patients. The aim of the present study was to evaluate physical activity level, health-related quality of life (HRQL) and perceived health three to six years after an acute myocardial infarction or an episode of unstable angina pectoris in elderly patients and compare this to earlier follow-up examination data. METHOD STUDY DESIGN: three to six years (mean 4.4 years) after randomization a questionnaire about physical activity level, HRQL and perceived health was mailed to all subjects alive (n = 93) (response rate 96%). The original study population consisted of 101 patients aged > or = 65 years admitted to the Coronary Care Unit at the Karolinska Hospital because of an acute coronary event between 1994 and 1997. The patients were randomized to either a three-month period of aerobic group training three times a week (n = 50; acute myocardial infarction 29, unstable angina pectoris 21) or served as control subjects (n = 51; acute myocardial infarction 31, unstable angina pectoris 20). RESULTS: Since the one-year follow-up examination eight subjects had died: five in the intervention group and three in the control group. At randomization, the level of physical activity level was similar in the two groups. Those in the intervention group improved their level of physical activity significantly over time (p = 0.05) in contrast to the control group. Measurements of HRQL showed no statistical difference between the groups by use of the EuroQol instrument. However, a small advantage for the intervention group measured over time was noted. CONCLUSION: It is important to include elderly patients in cardiac rehabilitation programmes after an acute coronary event, since even a short period of supervised exercise training has the potential to positively influence physical activity level for as long as three to six years.

Aged↗

PubMed2003

Influence of weight loss programmes on walking speed and relative oxygen cost (%VO2max) in obese women during walking.

OBJECTIVE: The objective was to investigate effects of dieted weight reduction on walking ability in obese women. METHODS: Fifty-seven obese women 44.1 +/- 10.7 years, body mass index 37.1 +/- 3.4 kg x m(-2) performed an indoor walking test. Speed, oxygen consumption and heart rate were measured, perceived exertion and pain graded and oxygen cost calculated. Maximum oxygen uptake (VO2max/kg) was predicted from a submaximum bicycle ergometry test. All tests were measured at baseline, after 12 weeks' weight reduction and after 52 weeks' maintenance. RESULTS: Despite a partial weight relapse, improvements were seen in body mass index, self-selected walking speed, VO2max/kg, heart rate, perceived exertion and relative oxygen cost of walking (% VO2max). CONCLUSION: A modest weight reduction of 10% in moderately-to-severely obese women significantly improved their walking ability, perceived exertion and %VO2max. When dealing with obese women, attention should be drawn to these positive effects, instead of focusing only on the magnitude of the weight loss.

Adult↗

PubMed2003

Peptidoglycan induces mobilization of the surface marker for activation marker CD66b in human neutrophils but not in eosinophils.

Peptidoglycan from Staphylococcus aureus mobilized CD66b in human neutrophils but did not upregulate surface activation markers in eosinophils. In addition, Toll-like receptor 2, implicated in the recognition of peptidoglycan, was detected on the surface of resting neutrophils but not on eosinophils. These findings suggest roles for neutrophils but not eosinophils in innate recognition of peptidoglycan.

Antigens, CD↗

PubMed2002

Identification of two protein-binding and functional regions of curli, a surface organelle and virulence determinant of Escherichia coli.

Curli are surface organelles of Escherichia coli. These fibrous proteins, formed by polymerization of a 15-kDa subunit, are expressed by E. coli strains associated with severe infections in humans. A remarkable property of curli is their ability to interact with a wide range of human proteins, interactions that contribute to the enhanced virulence of curli-expressing E. coli. To define the protein-binding region(s) of curli, we investigated the binding properties of overlapping synthetic peptides covering the curli subunit. Two peptides, one covering a 24-amino acid residue sequence in the NH(2)-terminal half of the subunit (NNS24) and one corresponding to the 26 COOH-terminal residues (VDQ26), were found to bind a number of human proteins. Physiochemical analysis revealed that NNS24 adopts a thermally stable beta-structure, and in solution the peptide forms soluble multimers, predominantly octamers. Intact curli are known to activate the proinflammatory and procoagulant contact system, and when added to human plasma, the NNS24 and VDQ26 peptides induced the release of the potent vasoactive peptide bradykinin. The results map important curli functions to the regions corresponding to the NNS24 and VDQ26 sequences.

Amino Acid Sequence↗

PubMed2002

Influence of interelectrode distance and force level on the spectral parameters of surface electromyographic recordings from the lumbar muscles.

In order to study the influence of interelectrode distance and force level on the electromyographic (EMG) spectral parameters and on their reliability, bipolar surface EMG measurements were performed on the lumbar muscles of 15 subjects. Two test contractions (45 s) at 40% of maximal voluntary contraction (MVC) were performed, one with 2 cm interelectrode distance and the other with 4 cm, followed by two contractions at 80% MVC with the same change in interelectrode distance. Increasing the interelectrode distance from 2 to 4 cm caused a significant mean decrease (about 8%) in the initial median frequency. It is shown that this shift is of an order of magnitude that may be expected from the bipolar electrode filter factor, and we further conclude that the observed individual variations in the shift are likely to be connected to fluctuations in the shape of the power spectrum and to variations in conduction velocity. No significant change was found for the median frequency slope when changing the interelectrode distance. Increasing the force (from 40 to 80% MVC) also caused a significant mean decrease (about 10%) in the initial median frequency. The median frequency slope became significantly more negative by more than 200%. We conclude, however, that torque fluctuations during the fatigue contractions should have had only minor influence on the standard error of measurement of the initial median frequency and of the median frequency slope.

Adult↗

PubMed2002

Physical capacity in non-ambulatory people with Duchenne muscular dystrophy or spinal muscular atrophy: a longitudinal study.

The purpose of this study was to describe functional ability, muscle strength, forced vital capacity, and clinical events in participants with Duchenne muscular dystrophy (DMD) or spinal muscular atrophy (SMA) in the non-ambulatory stages of the diseases. Nineteen non-ambulatory participants with DMD (all males; 13 to 24 years) and 13 with SMA (six males, seven females; 11 to 57 years) were assessed once a year over 5 years. The assessments comprised functional ability measured with the EK scale and upper extremity grade, muscle strength measured with the manual muscle test, and forced vital capacity defined as a percentage of normal values (FVC%). In the DMD group all variables measured deteriorated and there was a direct correlation between them. In the SMA group only muscle strength and FVC% deteriorated and there was no close relation between the variables measured. In the DMD group, 16 participants had cardiorespiratory clinical events leading to death in five cases. In the SMA group only four participants had respiratory clinical events, none leading to death. Although the participants with SMA had been extremely weak and non-ambulatory since early childhood they were older and less exposed to life-threatening events than the participants with DMD.

Activities of Daily Living↗

PubMed2002

Peptidoglycan from Staphylococcus aureus induces tissue factor expression and procoagulant activity in human monocytes.

Staphylococcus aureus is one of the most significant pathogens in human sepsis and endocarditis. S. aureus can initiate blood coagulation, leading to the formation of microthrombi and multiorgan dysfunction in sepsis, whereas in endocarditis the bacterium induces fibrin clots on the inner surface of the heart, so-called endocardial vegetations. In the present study, we show that live and heat-killed S. aureus bacteria are potent inducers of procoagulant activity in human peripheral blood mononuclear cells. Furthermore, purified peptidoglycan, the main cell wall component of S. aureus, induced procoagulant activity in mononuclear cells in a concentration-dependent fashion. The procoagulant activity in these cells was dependent on expression of tissue factor, since antibodies to tissue factor inhibited the effect of peptidoglycan. In mononuclear cells stimulated with peptidoglycan, reverse transcription-PCR showed tissue factor gene expression, and the gene product was detected by enzyme-linked immunosorbent assay. Finally, flow cytometry identified tissue factor at the surface of CD14-positive monocytes. Peptidoglycan is known to induce proinflammatory cytokine production in monocytes. The present investigation shows that peptidoglycan also activates the extrinsic pathway of coagulation by inducing the expression of tissue factor in these cells. This mechanism helps to explain the procoagulant activity, which plays such an important role in the pathogenicity of severe S. aureus infections.

Blood Coagulation Factors↗