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Biomedical subjects

F A Bautz

Publications and source records attributed to F A Bautz.

At least 37 records · Page 2Linked to original sources
PubMed1982

Cross-reaction of hnRNP-proteins of HeLa cells with nuclear proteins of Drosophila melanogaster demonstrated by a monoclonal antibody.

A monoclonal antibody from clone T7 raised against total nuclear proteins from the Kc cell line of Drosophila melanogaster (Saumweber, H. Symmons. P. Kabisch, R. Will, H & Bonhoeffer, F, Chromosoma 80 (1980) 253) [1] showed positive immunofluorescent staining on interphase nuclei of HeLa and PTK2 cells. When this antibody was allowed to react with several nuclear protein fractions isolated from HeLa S3 cells, three polypeptides of molecular weights (MW) 44 000, 63 000 and 70 000 were identified as the corresponding antigens, all components of hnRNA containing ribonucleoprotein particles. Sucrose gradient fractionation of such particles after mild RNase treatment and subsequent analysis of the proteins by the immunoblotting method revealed that the 44 000 MW antigen was an integral part of the ribonuclease-resistant complex. The results support the view that hnRNA molecules are associated with certain proteins conserved during evolution which may play structural roles in the ribonucleoprotein organization.

Animals↗
PubMed1980

Monoclonal antibody directed against RNA polymerase II of Drosophila melanogaster.

Monoclonal antibodies were raised against purified RNA polymerase II ( or B) from Drosophila melanogaster. The antibody produced by one hybridoma cell clone was found to be directed against the two large subunits of the enzyme. The absence of antibodies directed against proteins possibly contaminating the antigens used for immunization allowed us to identify RNA polymerase unequivocally in interbands and puffs of polytene chromosomes. Within a single heat shock puff (87C1) RNA polymerase was found to be clustered in two separate areas suggesting two distinct regions of RNA polymerase activity in this puff.

Animals↗
PubMed1980

[Possible therapy with and limits of gene technology (author's transl)].

This paper is an attempt to discuss hopes and limits of recent advances in genetechnology especially in respect of their medical applications. Although advances in modern biology are enormous no realistic prognoses can be given at the moment to their medical application in correcting inborn errors of metabolism at the level of the gene. However the production by fermentation of biosubstances like insulin, interferon and hormones, just to name a few, is not only possible but already carried out in many laboratories in USA. Therefore therapy of clinical manifestations like metabolic disorders, viral infections and hormone insufficiencies should become feasible based on the nearly unlimited in vitro production of the necessary compounds.

Animals↗
PubMed1979

Fluorescent phallotoxin, a tool for the visualization of cellular actin.

A fluorescent derivative of phalloidin has been synthesized possessing high affinity to filamentous actin. This compound was used for visualization of actin-containing structures in eukaryotic nonmuscle cells. Due to its low molecular weight (1250), fixation for formaldehyde was sufficient to render the membrane permeable for the labeled peptide. Bundles of microfilaments are the predominant pattern in the flat rat kangaroo PtK1 cells, whereas a net of concentric fibers characterizes the more spherical bovine kidney MDBK cells. Specificity of staining was confirmed by competition experiments with unlabeled phalloidin.

Actins↗