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Biomedical subjects

F A García

Publications and source records attributed to F A García.

13 recordsLinked to original sources
PubMed1999

Reconstruction of the eyelids of a dog using grafts of oral mucosa.

A five-month-old English cocker spaniel with distichiasis had been treated four times surgically by a tarsomeibomian resection technique. As a result, three-quarters of the margins of its upper eyelids had been eliminated and the lower eyelids had become considerably thinner. The dog had developed upper entropion and upper eyelid trichiasis, and keratoconjunctivitis sicca. It was treated medically and grafts of oral mucosa were used to reconstruct its upper eyelids. A year after surgery, the new palpebral margin was completely functional and cosmetically acceptable. The dog's eyes appear normal and only require treatment with artificial tears.

Animals↗
PubMed1998

Enzyme-linked immunosorbent assay (ELISA) for detection of anti-Trypanosoma evansi equine antibodies.

The standardization of ELISA for the detection of anti-Trypanosoma evansi antibodies in naturally and experimentally infected horses is described. Bayesian analysis was used to establish the cutoff between positive and negative sera. In order to determine the assessment of the ELISA test, the results obtained were compared with those from an IFA. A relative sensibility of 98.39%, a specificity of 95.12% and a predictive value of 96.83% were determined. The standardized technique was used to evaluate the antibody production against trypanosome in an experimentally infected equine, in which the sera converted 15 days after infection. The test was also used for a study of sera prevalence in a non-random sample from two different populations. A prevalence of 81.7% in workhorse and 57.14% in stable horses was found.

Animals↗
PubMed1998

[Neurosyphilis and the prozone effect].

INTRODUCTION: Neurosyphilis (NS) is an entity which still frequently presents to our Neurology Department. The prozone phenomenon occurs in approximately 2% of all cases of late primary syphilis or secondary syphilis; we have found no cases described of prozone and neurosyphilis occurring together. CLINICAL CASE: We present the unusual case of a 44 year old patient with NS and dementia PGP (progressive general paralysis). Initially serum VDRL was negative, but in CSF reacted at dilutions of 1:32. When serum VDRL was repeated using dilutions, it was reactive 1:128 and serum FTA was also reactive. The patient was treated with i.v. crystalline penicillin, after which his condition improved. CONCLUSIONS: We wish to draw attention to the possibility that patients with a dementia syndrome and negative serum VDRL may have the prozone phenomenon, and the laboratory should therefore be asked to do serial dilutions.

Adult↗
PubMed1997

A new type of intraocular prosthesis for dogs.

A simple technique is described in which sterile silicone oil (viscosity 350 cSt) was injected into the globe of an eviscerated eye with an intact cornea and sclera; the volume injected was calculated from the formula 3/4 pi r3 where 'r' was the horizontal corneal diameter. After induction of general anaesthesia and routine preparation of the surgical site, the globe was eviscerated by using a transscleral or transcorneal approach. The procedure was carried out in five dogs with follow-ups ranging between 19 and 27 months. None of the eyes developed postoperative complications. In one dog, more silicone oil had to be injected 10 days after surgery to increase the size of the globe to match the other eye. The intraocular contents were removed more easily by using the transcorneal approach, which also resulted in a perfect adjustment and virtually eliminated the possibility of leakage of silicone oil, than by a transscleral approach. The dogs responded extremely well and their owners were satisfied with the cosmetic appearance of their pets.

Animals↗
PubMed1994

[Status of insecticide resistance in 4 strains of Musca domestica collected in animal farms].

Resistance to insecticides made from different chemical compounds were studied in 4 strains of Musca domestica collected from 2 poultry farms and 2 cow farms following WHO methodology. A high resistance to malathion was observed (resistance factor values ranging from 102X to 252X), and moderate, to diazinon, fenthion, dichlorvos, DDT, and propoxur. Resistance to the deltametrine and permethrin pyreturins was not observed.

Animal Husbandry↗
PubMed1986

[Proteolytic susceptibility of alpha-amylase of the pancreas of swine deprived of its structural calcium].

Hog pancreas alpha-amylase (alpha-1-4-glucan-glucan hydrolase, E.C. 3.2.1.1) lost its structural calcium by action of EDTA at 20 degrees C. Enzymatic activity experimented a decrease whereas a big increase in proteolytic susceptibility to bovine pancreas trypsin (E.C. 3.4.4.4) was shown. Native alpha-amylase had an activity of 2,730 mg maltose/min X mg enzyme and a Km of 0.222% amylose, the activity of calcium depleted amylase being of 1,640 mg maltose/min X mg enzyme and Km 0.571% amylose. Simple methods for evaluating proteolytic susceptibility of alpha-amylase micro-amounts against trypsin action, and for the measurement of alpha-amylase activity in polyacrylamide rod gels were also described.

Animals↗
PubMedDate not supplied

[Comparison of 3 methods for collecting adult anophelines in a risk area of malaria transmission].

Three methods of collecting adult anophelines were comparatively studied in a rural town in Havana Province, during the period comprised between June and September 1986. The methods of capture used were as follows: in resting sites, in human bait and through lactic acid CDC light traps. A greater number of anophelines (F = 6.86; p less than 0.01) was collected on human bait. The capture of 11 species of culicines was reported.

Animals↗