PubMed Health⌕ Search

Biomedical subjects

F A Gusberti

Publications and source records attributed to F A Gusberti.

23 records · Page 2Linked to original sources
PubMed1984

Oxygen tension (pO2) in untreated human periodontal pockets.

The purpose of this study was to assess the oxygen tension in untreated human periodontal pockets and test the hypothesis that the subgingival environmental is anaerobic in nature. Twenty-six patients with advanced chronic inflammatory periodontal disease participated. A total of 111 untreated pockets, 5 to 10 mm in depth, were selected for the pO2 measurements. Pocket depths, Plaque-Index and Gingival-Index were recorded. The pO2 at the base of the 111 pockets ranged from 5 to 27 mm Hg, with an average of 13.3 mm Hg (1.8% O2). Mean pocket depth was 6.9 mm. Moderately deep pockets (5-6 mm) had a mean pO2 of 15.0 mm Hg, whereas deep pockets (7-10 mm) showed a significantly lower pO2 of 11.6 mm Hg. No correlation was found between the pO2 and the Plaque-Index. Higher Gingival-Index scores tended to be associated with higher pO2 values. The pO2 in untreated periodontal pockets was low. However, it does not represent a completely anaerobic environment. Deep pockets contained less oxygen than moderately deep sites.

Adult↗
PubMed1983

Puberty gingivitis in insulin-dependent diabetic children. I. Cross-sectional observations.

This cross-sectional study examined the gingivitis occurring at puberty in a population of insulin-dependent juvenile diabetics. Seventy-seven children between the ages of 6 and 15 years were examined for gingivitis levels, stages of pubertal maturation and blood levels of glucose and glycosylated hemoglobin. Bacterial plaque was sampled from one or more approximal tooth surfaces of every subject and cultured under anaerobic and aerobic conditions on nonselective and selective media. The total cultivable flora and percentage of certain presumptive periodontopathic bacteria were determined. Before puberty, children with "high" levels of glycosylated hemoglobin also had higher gingivitis levels than children with "normal" metabolic control of diabetes. During puberty, the level of gingivitis increased independently from both fasting blood glucose levels and per cent glycosylated hemoglobin. The microbiota of marginal plaque was predominantly composed of facultatively anaerobic bacteria. The percentages of Capnocytophaga sp and Actinomyces naeslundii were statistically higher at the onset of puberty, suggesting that a specific bacterial shift in the microbial composition of marginal plaque occurs in response to host changes in juvenile diabetic children at this age period.

Adolescent↗
PubMedDate not supplied

In vivo early human dental plaque formation on different supporting substances. A scanning electron microscopic and bacteriological study.

Different studies have shown that various substances may have an influence on early human dental plaque formation. The purpose of the present study was to compare on tooth substances and supporting prosthetic materials the amount of plaque deposition by SEM and the quantity of selected bacteria using anaerobic culturing techniques. 5 bridges, replacing a missing molar or premolar, were incorporated in 3 patients. In the midbuccal area of each pontic, a semi-precision attachment was placed allowing the insertion of the following test facings: enamel, dentine, non gamma 2-amalgam, alloys of 85% and 55% gold, silver-palladium, chrome-cobalt, chrome-cobalt-titanium, and ceramic. For each material, 2 facings were fabricated. After 4 and 24 hours in situ, bacteriological samples were taken and processed for further identification. After a 2nd period of 4 and 24 hours in situ, the same facings were carefully removed and prepared for SEM-examination. All 4-hour specimens exhibited various areas covered by plaque, the amount of which varied with the different supporting substances. The very smooth surfaces (e.g., gold) harbored sparse deposits, while the rougher (e.g., amalgam) were covered by more plaque. After 24 hours of plaque development, an increase in the number of micro-organisms was noted for all the specimens. After 4 and 24 hours of plaque accumulation, no specific trends suggesting a preferential colonization on the different substances were observed. This study has shown that the amount of early deposits on different substances seems to be related to the degree of their surface roughness, while plaque formation was qualitatively similar.

Adult↗