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F A Navarro

Publications and source records attributed to F A Navarro.

8 recordsLinked to original sources
PubMed1992

Is the monoclonal fluorescence polarization immunoassay for cyclosporine specific? Comparison with specific radioimmunoassay.

Trough concentrations of cyclosporine (CsA) in whole blood were measured by specific monoclonal 3H-radioimmunoassay (S-RIA) and compared with those obtained by monoclonal fluorescence polarization immunoassay (S-FPIA) in 89 transplant recipients. Differences in the S-RIA:S-FPIA relationship between kidney transplant (KT, n = 59) and heart transplant (HT, n = 24) recipients were investigated. Mean concentrations of CsA were significantly higher by S-FPIA than by S-RIA [191 +/- 127 vs. 166 +/- 124 ng/ml (mean +/- SD), p less than 0.001], with a S-FPIA:S-RIA ratio of 1.25 +/- 0.33. Higher ratios were observed either in patients with low CsA concentrations or in those with a high proportion of metabolites. HT recipients had higher S-FPIA:S-RIA ratios than did KT recipients (1.37 vs. 1.22, t = 1.87, p = 0.065). Within-assay coefficients of variation were lower for S-FPIA than for S-RIA (2.4% vs. 12.3%, p less than 0.001). Monoclonal FPIA is a precise and fast method, more suitable than RIA for therapeutic CsA monitoring in clinical practice. However, our results indicate a 25% higher mean CsA concentration by S-FPIA than by S-RIA (37% in HT recipients), which should be borne in mind until therapeutic and toxic ranges are established or a more specific FPIA method is developed.

Antibodies, Monoclonal↗