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Biomedical subjects

F Abbas

Publications and source records attributed to F Abbas.

85 records · Page 5Linked to original sources
PubMed1985

Experimental gingivitis in relation to susceptibility to periodontal disease. (I.) Clinical observations.

The aim of the present study was to investigate the rate of development of experimentally-induced gingival inflammation in relation to the susceptibility to periodontal disease. By selection according to age, a younger (25-39 years) and an older (45-54 years) age group, with a comparable reduced but healthy periodontium, was selected. This equal amount of periodontal breakdown may suggest that the younger age group represented individuals with a relatively higher degree of susceptibility to periodontal disease. At the start of the experiment, each patient was instructed to abstain from oral hygiene procedures in 1 quadrant of the mouth for a period of 18 days. Results showed that all subjects developed signs of gingival inflammation. Regarding the development of redness and swelling, no differences could be assessed between the 2 age groups. However, analysis of the bleeding scores revealed that bleeding on probing developed more rapidly in the younger age group. It was concluded that those patients who have suffered from a more rapid form of periodontal disease also develop inflammation, in terms of bleeding on probing, more rapidly.

Adult↗
PubMed1985

Bleeding/plaque ratio. A possible prognostic indicator for periodontal breakdown.

The purpose of the present study was to investigate the clinical differences between individuals highly susceptible and individuals insusceptible to periodontal breakdown. The susceptible group consisted of 7 patients with a diagnosis of juvenile periodontitis. The insusceptible group consisted of 7 individuals selected on the basis of age (52 years or older), presence of at least 18 teeth, no evidence of extractions due to periodontal breakdown, no loss of attachment, shallow pockets, gross amounts of plaque and no history of interdental cleaning. Results showed that the susceptible group had more bleeding, less plaque and deeper pockets than the insusceptible group. Since the 2 groups seem to behave differently with regard to bleeding upon probing and amount of plaque, bleeding/plaque ratios were calculated. Testing showed a highly significant difference between the 2 groups. The same results were obtained if only sites with shallow pockets were included in the analysis. On the basis of these results, it is hypothesized that a high value of the ratio between bleeding and plaque may act as a prognostic indicator for periodontal breakdown.

Adolescent↗
PubMed1984

Relation between wound healing after surgery and susceptibility to periodontal disease.

In the present investigation wound healing was studied clinically in 8 younger (mean age 33.5 years) and 8 older patients (mean age 48 years), who were surgically treated for the same amount of severe periodontitis. This implies that the patients in the younger age group represented individuals with a higher degree of susceptibility to periodontal disease than the patients in the older age group. After surgery all patients were subjected to a carefully controlled oral hygiene program. Patients were recalled weekly until 8 weeks post surgery and again after 15 weeks for a final examination. At every recall session oral hygiene measurements were carried out and the bleeding tendency of the pockets was determined after probing with a standardized pressure. Bleeding on the basis of mechanical trauma after probing was considered to be a clinical parameter for wound healing in a plaque free environment. Results indicate that the oral hygiene program resulted in equally low plaque scores in both age groups. However, in a period from 5-15 weeks after surgery younger patients showed significantly more bleeding pockets that older patients. Furthermore it was found in both groups that the more loss of attachment there was, the slower the rate of wound healing. It was concluded that the time span for wound healing is longer in patients who are more susceptible to periodontal disease, than in those who are less susceptible.

Adult↗
PubMedDate not supplied

Characterization of monoclonal antibodies against infections laryngotracheitis virus.

Nine monoclonal antibodies (MCAs) produced against two different strains of infectious laryngotracheitis virus (ILTV) were characterized and compared to previously characterized MCA 131-6, produced against a third ILTV strain. In western blotting experiments, MCAs C, E, and 11 resembled MCA 131-6, detecting proteins of 205, 160, 115, and 90 kD as well as several proteins less than 49 kD. The other six MCAs differed from previously described ILTV MCA. MCA D detected a 90-kD protein along with several less than 49 kD. MCAs 4 and 5 each detected proteins of 205, 160, 100, 90, and 70 kD. MCA 9 detected the same proteins detected by MCAs 4 and 5 except the 160-kD protein. MCA 10 detected proteins of 100, 90, and 70 kD and several proteins less than 49 kD. MCAs C, D, and E, like MCA 131-6, failed to react with any ILTV grown in the presence of tunicamycin, suggesting that those MCAs are specific for carbohydrate-based epitopes. MCA 6 reacted with only a 100-kD protein in the presence or absence of tunicamycin. The remaining MCA detected only a 70-kD protein in the presence of tunicamycin except MCA 5, which reacted with proteins of 70 and 90 kD. Only MCA 4 and 6 neutralized ILTV infectivity.

Animals↗
PubMedDate not supplied

Development of a polymerase chain reaction and a nonradioactive DNA probe for infectious laryngotracheitis virus.

The polymerase chain reaction (PCR) was developed using infectious laryngotracheitis virus (ILTV) primers made from a portion of the ILTV thymidine kinase gene. DNA from various ILTV field isolates, from the USDA challenge strain of ILTV, and from commercial ILTV vaccines was specifically amplified. No amplification occurred using template DNA from uninfected chicken-embryo liver cells (CELC), several nonavian alphaher-pesviruses, Mycoplasma gallisepticum, Mycoplasma synoviae, Pasteurella hemolytica, Escherichia coli, a group I avian adenovirus, fowl poxvirus, or a psittacid herpesvirus. The 647-base pair-amplified ILTV PCR product was labeled to create a nonradioactive, biotinylated DNA probe. Hybridization using the probe detected ILTV DNA. Both PCR and hybridization yielded positive results with ILTV DNA but not with the DNA of other pathogens. Hybridization was specific for ILTV using a stringent salt solution for a 30-min wash step or a somewhat less stringent salt solution for a 60-min wash step. However, slight hybridization occurred with CELC DNA when the less stringent salt solution was used in a 30-min wash step.

Animals↗
PubMedDate not supplied

Comparison of diagnostic tests for infectious laryngotracheitis.

Diagnostic procedures for detection of infectious laryngotracheitis virus in tracheas of experimentally infected chickens, including the indirect fluorescent antibody test (IFAT), immunoperoxidase (IP), virus isolation (VI), histopathology, polymerase chain reaction (PCR), and DNA hybridization, were performed and compared. Using VI as a reference, we calculated the sensitivity and specificity of the tests. The sensitivities of IP, IFAT, histopathology, PCR, and hybridization were 100%, 93%, 7%, 27%, and 0%, respectively, and the specificities of IP, IFAT, histopathology, PCR, and hybridization were 93%, 93%, 100%, 100%, and 100%, respectively. Histopathology, PCR, and hybridization were more specific but lacked sensitivity compared to IP and IFAT. IP and IFAT were equally specific, but IP was more sensitive than IFAT. Based on these results, IP performed better than any other test.

Animals↗