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Biomedical subjects

F Altomonte

Publications and source records attributed to F Altomonte.

7 recordsLinked to original sources
PubMed1991

A new glucose clamp algorithm: clinical validation.

A new glucose clamp technique for in vivo studies of insulin sensitivity was validated clinically. Eighteen patients (10 males, 8 females, age 35-80 years, body mass index 34.6-17.04) were connected to a computer-assisted artificial pancreas "Betalike R", using a new algorithm based on a "minimal model", to carry out the glucose clamp technique automatically and especially to overcome the well-known problems of its priming phase. We performed the euglycemic hyperinsulinemic clamp in four patients and the hyperglycemic hyperinsulinemic clamp in 14. In one patient both clamps were done. The mean priming time to reach steady-state glycemia was 20 min. Plasma insulin concentrations were measured every 20 min. This new automatic glucose clamp technique enables the priming phase to be run without any significant overshoot, and accidental variations of glycemia in steady state were reduced to a minimum. The system showed satisfactory safety and stability in controlling the patient's glycemia and assured high speed of the priming phase.

Adult↗
PubMed1991

[Evaluation of a new immunoturbidimetry technique for measuring microalbuminuria].

Subclinical elevation of urinary albumin excretion is a good predictor of later clinical proteinuria. A simple, sensitive and rapid immunoturbidimetric method was developed to quantify urinary albumin excretion (URIN-PAK ImmunoMICRO LAB, Miles Italia Spa). In the presence of polyethylene glycol 6000, immunocomplex between human albumin and its specific antibody are rapidly formed (5-50 min, at room temperature). Absorbance reading are mode U 340 nm (Automatic Analyzer RA 1000, Technicon). The test is specific for albumin failing to cross react with other plasma proteins present in urine, as well as with glibenclamide, chlorpropamide, phenformin, hemoglobin, glucose, urea and thymol. The present method correlates with SCLAVO H-ALBUMIN RIA Kit (r = 0.9917). The test is suitable for clinical use.

Albuminuria↗
PubMed1985

[Cardioinhibitory reflex provoked by stimulation of carotid sinus in normal subjects and those with cardiovascular disease].

The normal range and the reproducibility of the cardioinhibitory carotid sinus reflex were studied in 288 apparently healthy subjects of different ages (aged from 17 to 84 yrs., 156 males, 132 females). In each subject we chose the longest RR interval as an activation index of the reflex obtained by carotid sinus massage; its mean value increased slightly with advancing age. In the were 1, 5, 2, 5, 3, 3 sec. respectively, with a 99% confidence limit. No sex difference was found. We studied also a group of 105 patients (aged from 39 to 82 yrs., 67 males, 38 females) with various types of cardiovascular disorders, without a previous history of spontaneous syncope. Abnormal maximum RR values were found in 18 of them (17%). This percentage was significantly higher than in normals (2%). In 11 patients (10%) the carotid sinus stimulation evoked a syncopal attack. It seems therefore that a statistically abnormal carotid sinus hypersensitivity can occur in a substantial number of cardiovascular patients without necessarily leading to spontaneous syncopal attacks. Finally the reproducibility of the cardioinhibitory reflex was tested in 42 patients with maximum RR interval values ranging from normal to extremely abnormal. In each subject a significant correlation (r = 0.79) was found between the maximum RR interval values tested in two occasions at intervals ranging from 1 hour to 3 months; besides a concordance in normal or abnormal result was present in 93% of the cases.

Adolescent↗