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Biomedical subjects

F Anfosso

Publications and source records attributed to F Anfosso.

33 records · Page 2Linked to original sources
PubMed1980

Pollen-allergen IgE response in the inbred rat. Regulation of allergen P-IgE antibodies in high and low responders by suppressive factors.

Cyclophosphamide treatment (100 mg/kg) significantly increased the IgE response to plane-tree allergen P in low-responder strains of rats. A suppressive serum was obtained by injection of CFA in normal low-responder strains. The injection of this serum 2 days after cyclophosphamide treatment and 1 day prior to primary immunization, reversed the cyclophosphamide-enhanced IgE antibodies response without affecting the IgG2a levels. This effect is strain specific.

Allergens↗
PubMed1979

[Are rat alveolar macrophages capable of phagocytizing pollen grains?].

Plane-tree pollen grains were incubated in vitro with alveolar macrophages from inbred Rats, and the possibility of phagocytosis was investigated. No phagocytosis was observed even after 48 hrs incubation. But alveolar cells were bound to pollen grains generally at the apertures. This binding did not require the Fc receptor on the macrophage membrane.

Animals↗
PubMed1979

Cyclic AMP in hypersensitivity reactions.

Phytohemagglutinin (PHA) was capable of inducing non-cytotoxic histamine release from human leucocytes. In the presence of deuterium oxide (D2O), PHA caused significantly greater histamine release. Dibutyryl cyclic AMP (d-cAMP) could enhance the histamine release in the presence of D2O although it was an inhibitor of the release if used alone. However, a beta agonist, isoproterenol, which increases intracellular level of cAMP was inhibitory with or without D2O. These data ask the question about dual effect of cAMP and suggest the possibility of different polls of cAMP in the target cells.

Basophils↗
PubMed1979

[Antifungal activity of aromatic essential oils (author's transl)].

"Paragerm" is commonly used in hospitals where its bactericide action is well known. Preliminary tests have been carried out in the laboratory in order to study the antifungal power of this product. Variation of the different concentrations in the culture medium, variation of the time of contact between "Paragerm" and the fungus and both the mechanical and chemical effects of the product have shown that, among the species selected, certain were more sensitive and others more resistant to this solution and that, in our experimentation, "Paragerm" had a fungistatic effect.

Air Microbiology↗
PubMed1979

Denaturization of allergen P: effect on allergenicity, antigenicity and immunogenicity.

When allergen P was denatured by 8M urea, the modified molecule still reacted with IgE specific for the native allergen but not with hemagglutinating antibodies. Heating at 100 degrees C abolished the reaction in both cases. The results suggested differences between allergenic and antigenic capacities which may be based on structural differences of the antigenic determinants.

Allergens↗
PubMed1979

Drug allergy: in vitro cross-allergenicity between amoxicillin and benzyl penicillin.

The capacity of amoxicillin to elicit allergenic reactions in vitro was determined on benzyl-penicillin sensitive patients. RAST and histamine release were performed on blood of these patients using B pen linked to human serum albumin (BPO-HSA) as allergen. A relationship was noted between the results of the in vitro tests and the date of the allergic manifestations. When results of RAST and histamine release were significant, amoxicillin was tested for its capacity to elicit histamine release and to inhibit RAST performed with BPO-HSA. A cross-allergenicity was observed in six sera out of eight. The results suggested that amoxicillin may be capable to react with cell-bound or serum IgE to give hypersensitive manifestations in some penicillin sensitive patients.

Allergens↗
PubMedDate not supplied

Stimulating effect of oxidized low density lipoproteins on plasminogen activator inhibitor-1 synthesis by endothelial cells.

Oxidized low density lipoproteins (ox-LDL) are thought to accelerate atherogenesis. It was recently demonstrated that patients with coronary heart disease have defects in plasma fibrinolysis due to increased plasminogen activator inhibitor-1 (PAI-1) levels. Investigation of PAI-1 synthesis by endothelial cells may allow insight into the effect of native LDL (N-LDL) and ox-LDL on endothelial cells. In the present study, secretion of PAI-1 by human umbilical vein endothelial cells (HUVEC) in culture was evaluated after incubation with N-LDL and ox-LDL. Ox-LDL were obtained by peroxidation under ultraviolet radiation, which induced compositional changes in LDL, namely, a decrease in the levels of arachidonic acid, eicosapentaenoic acid, docosahexaenoic acid, and alpha-tocopherol and an increase in the malondialdehyde content. Ox-LDL induced a dose-dependent increase in PAI-1 secretion by HUVEC as assayed by an enzyme-linked immunosorbent assay. After a 24-hour incubation, a twofold increase in the PAI-1 content was observed with 50 micrograms/ml ox-LDL protein. Studies with inhibitors of protein synthesis and metabolic labeling with [35S]methionine confirmed that PAI-1 synthesis was stimulated by ox-LDL. N-LDL had no detectable effect on PAI-1 secretion. Binding studies with radiolabeled lipoproteins showed that the effect of ox-LDL was independent of the B/E receptor. Our experiments indicate that ox-LDL stimulate PAI-1 secretion from HUVEC and that this effect may involve a scavenger receptor.

Binding, Competitive↗
PubMedDate not supplied

Specific and non-specific stimulation of unprimed and primed lymphocytes induced by plane-tree allergenic extracts in rats.

Whole allergenic extracts (WAE) from plane-tree pollen induces in vitro blastogenesis in splenic lymphocytes from primed and normal rats. Allergen P, the major allergen of plane-tree pollen, induces an early stimulation in primed cells. WAE induced in the same way an early stimulation in primed cells, but a late incorporation of 3H-thymidine occurs at 8 days of culture when lymphocytes from primed or normal rats were used. This late response appears to be non-specific and is not abolished when a short-pulse of antigen is given.

Allergens↗