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F Arai

Publications and source records attributed to F Arai.

51 records · Page 3Linked to original sources
PubMed1983

[Biliary excretion of diethyl lead after administration of tetraethyl lead in rabbits].

In order to investigate into whether the large amount of inorganic lead excreted into the feces following intravenous injection of tetraethyl lead to rabbits is derived from the diethyl lead excreted into the bile, we administered 12 mg/kg of tetraethyl lead to rabbits which had been fistulated into the bile duct for taking the bile out of the body. The total lead excreted into the bile during the first 24 hours after the injection of tetraethyl lead amounted to about 8% of the injected amount of lead (with 97% of the excreted lead made up of diethyl lead). The amount of total lead contained in the cecal contents of unfistulated rabbits 24 hours after the injection of tetraethyl lead was equivalent to about 12% of the injected amount of lead (with inorganic lead accounting for about 90% of the excreted lead), but the counterpart of the fistulated rabbits was equivalent only to about 0.6%. The amount of lead excreted into the bile, when measured in terms of the total lead content of the liver, was slightly less in the fistulated rabbits than in the unfistulated ones. These findings indicated that the amount of total lead excreted into the bile of the fistulated rabbits was almost the same as that contained in the cecal contents of unfistulated rabbits, and that the major portion of the lead contained in the cecal contents or feces was composed of inorganic lead. From these results, we came to the conclusion that the large amount of inorganic lead detected in the feces after the injection of tetraethyl lead is derived from the diethyl lead excreted into the bile.

Animals↗
PubMed1981

Plasma glucose concentrations of whole blood, as determined with a multilayer-film analytical element.

A new type of multilayer film analytical element--consisting of a spreading layer, a blocking layer, an enzymic reagent layer, and a transparent layer--has been developed for blood glucose determination. After a spot of whole blood is dropped on the film, the plasma glucose concentration is determined in 6 min without further manipulations. Precise measurement of sample volume spotted on the film is not necessary for good results, nor is knowledge of the hematocrit in the range 10-46%. The minimum concentration of glucose detectable by this method is 100 mg/L. The coefficients of variation, within-run and between-run, were all less than 2.5% for 800-4000 mg/L glucose concentrations. Macromolecules and hydrophobic substances in blood did not interfere. This method seems suitable for emergency use, especially.

Blood Glucose↗
PubMed1980

The relation between mercury levels in brain and blood or cerebrospinal fluid (CSF) after mercury exposure.

Rabbits were exposed to elemental mercury (Hg degrees) vapor, methyl mercury (MeHg) and HgCl2 respectively, and the relationship of mercury concentration in brain to that in blood or cerebrospinal fluid (CSF) was investigated after the termination of exposure. At one day post-exposure, the ratio of mercury concentration in brain (microgram Hg/g) to that in blood (microgram Hg/g) was approximately 10.0 in Hg degrees vapor, 0.6 in HgCl2 and 3.0 in MeHg. The ratio for Hg degrees vapor was about 10 times higher than that for HgCl2. In rabbits exposed to Hg degrees vapor, the ratios of mercury concentration in blood to brain or CSF to brain at 1 day and 20 days after the termination of exposure were compared. The blood to brain ratio showed a decrease to about 1/10 after 20 days, and the CSF to brain ratio, a decrease of about 1/2. The results obtained seems to suggest that mercury is eliminated from the blood and CSF more rapidly than from the brain. In the case of rabbits administrated MeHg, the ratio of mercury concentration in blood to that in brain was almost unchanged at both 1 day and 20 days after the termination of exposure. There did not seem to be any differences in the rates of elimination from blood and brain. The elimination of mercury from CSF was more rapid than from brain because the ratio between the mercury concentration of CSF to that of brain showed a decrease to about 1/3 after 20 days.

Animals↗
PubMedDate not supplied

Presence of dopamine DA-1 receptors in human decidua.

To evaluate the role of dopamine in human parturition, we studied the presence of dopamine receptors in human decidua obtained immediately after delivery, using a radiolabelled receptor assay. [3H]SCH23390, a dopamine DA-1 receptor ligand was used to identify and characterize the DA-1 receptor. Scatchard analysis of our saturation experiments revealed one class of high-affinity binding sites. The equilibrium dissociation constant (Kd) for binding of [3H]SCH23390 to decidual DA-1 receptors was 1.87 +/- 0.16 nM (mean +/- s.d. n = 3) at 30 degrees C. The maximum binding capacity (Bmax) was 79.0 +/- 15.4 fmol/mg protein at 30 degrees C. The specificity of DA-1 receptor binding was inhibited by SCH23390 (a DA-1 antagonist), SKF38393 (a DA-1 agonist) and dopamine with the inhibitory constants (Ki) of 5.82 +/- 2.27 nM (mean +/- s.d., n = 3), 45.6 +/- 30.5 nM (mean +/- s.d. n = 3) and 2.86 +/- 1.02 microM (mean +/- s.d., n = 3) respectively. However, (+/-)-sulpiride (a DA-2 antagonist) and (-)-propranolol (a beta-adrenergic antagonist) were without effect. The specific binding of [3H]spiperone (a DA-2 receptor ligand) was not detected. We previously reported that in vitro the human decidual tissues produce significant amounts of prostaglandins in the presence of dopamine. We postulate that dopamine regulates the production of prostaglandin in human decidua via DA-1 receptors.

Benzazepines↗