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Biomedical subjects

F Barnett

Publications and source records attributed to F Barnett.

34 records · Page 2Linked to original sources
PubMed1987

Fluorescent inhibitors of a cell surface protease used to locate leukaemia cells in kidney sections.

Guanidinobenzoatase is a trypsin-like protease on the surface of cells capable of migration, for example leukaemia cells. We have used a number of fluorescent probes that are competitive inhibitors of guanidinobenzoatase to locate leukaemia cells in resin sections of kidney tissue obtained from leukaemic rats. We have demonstrated how this competitive inhibition system can be used to direct desired molecules (such as cytotoxic drugs) to these cells and to monitor the arrival of such compounds at the active site of guanidinobenzoatase. The principles developed in this study could equally well be applied to other enzymes on other cells provided suitable competitive inhibitors were designed. The presence of an enzyme on the surface of a cell can be used to direct molecules to that cell provided that these molecules contain a functional group that acts as an inhibitor for the chosen enzyme.

Animals↗
PubMed1986

Is 2% hydroxypropylmethylcellulose a safe solution for intraoperative clinical applications?

We examined 2% hydroxypropylmethylcellulose prepared for clinical intraocular surgical procedures in various laboratories. Each sample contained a variety of particulate debris of botanical origin, although in varying amounts. The identified material was also seen in a sample of the raw material from which all the clinical material had been prepared. Our conclusion is that filtration methods, which are the physical methods used to purify the product, are at worst ineffective and at best inadequate. Until proper laboratory and clinical studies confirm an acceptable level of adverse reactions, we recommend that this material not be used clinically.

Anterior Eye Segment↗
PubMed1984

Tissue response to anodyne medicaments.

The tissue-irritating effect of anodyne medicaments was studied via enzyme histochemical evaluation of muscle tissue (screening test) and pulp tissue (usage test). Of the medicaments tested, formocresol was the most irritating, followed by Cresatin, eugenol, and oil of pimenta leaf. The effects of Cresatin, eugenol, and oil of pimenta leaf were not statistically distinguishable.

Animals↗
PubMed1975

Effectiveness of trabeculectomy in glaucoma.

A follow-up study of 90 eyes in 60 patients subjected to trabeculectomy between 1967 and 1972 showed that intraocular pressure was controlled at the onset in 84% of the eyes, and eventually controlled in over 97%. Only 11% of the eyes required further medication and 5.5% further surgery. Subconjunctival drainage was established by means of a bleb in 91%. Trabeculectomy produced a high significant fall in intraocular pressure (P less than .001) and a parallel rise in aqueous outflow facility. The absolute fall in intraocular pressure was constant whether the preoperative pressure was high or low and whether or not the postoperative pressure remained above 20 mm Hg. This method was virtually free of major operative and postoperative complications when used appropriately; and it can be modified during the operation to deal with peripheral anterior synechiae. The anterior chamber remained formed or, if lost, was speedily re-formed. The anterior chamber rarely flattened postoperatively.

Adult↗