Biomedical subjects

F Belleville

Publications and source records attributed to F Belleville.

At least 73 records · Page 4Linked to original sources

PubMed1977

[Glucose role in regulation of placental lactogen hormone (hPL) secretion].

The authors have studied the role of glucose on the excretion of hPL in the medium by explants of placenta in culture. 1. The glucose is necessary for this excretion and cannot be replaced by lactate. 2. At concentrations higher than 1.5 g/l, the glucose inhibits the excretion of hPL. 3. This inhibition is shown for long periods of action of the glucose (4 days) as well as for short periods (1, 2 and 4 hours).

Dose-Response Relationship, Drug↗

PubMed1977

[Circulating molecular forms of prolactin in the normal nursing mother and during tumoral amenorrhea-galactorrhea].

The authors have studied by gel chromatography and radioimmuno assay, the molecular forms of prolactine in the serum of normal lactating women and in serum of three subjects having an hypophyseal tumor. 1. In the normal women, one find two pics of prolactine : one migrating like the monomere (M.W. 20000), and the other as a trimere (M.W. : 60000). There is small amounts of a large molecule migrating in the dead volume of the column. 2. In the patients having a tumor, one find not only the monomere but also a large molecule (M.W. greater than 100000) which is the principal form in the serum. The results are compared with these of the literature.

Adenoma↗

PubMed1977

[Study of molecular forms of ovine and human prolactin].

After labelling of human and ovine prolactine by the lactoperoxydase method, each prolactine shows at least three molecular forms : amorphous aggregates of large molecular weight, one dimere (M.W. no. 50 000) and one monomere (M.W. no. 25 000). The monomere and the dimere can spontaneously give rise to the aggregates. But the dimere never give monomere and monomere never give dimere. Urea 8 M alone have not dissociating action on the polymeres or on the dimere. But urea 8 M plus mercaptoethanol transforms polymeres into dimeres and monomeres.

Animals↗

PubMed1977

[Cerebrospinal fluid distribution of metampicillin (author's transl)].

UNLABELLED: In order to avoid other antibiotics which interfere with plasma and CSF level determination of a given antibiotic the authors use a chemical method of established specificity. 15 patients presenting with purulent meningitis were given a standard daily dose of metampicillin and divided into 3 groups of 5. On days 2, 4 and 6 after onset of disease plasma and CSF specimens were taken at respectively 1, 2 or 4 hours following metampicillin I.V. injection according to patient group. All patients were cured between days 7 and 21 of therapy. Tolerance was good. RESULTS: --there was no correlation between dose injected and plasma level of drug,--CSF distribution of metampicillin was good and stayed identical throughout the disease period,--peak CSF drug level was delayed as compared to plasma level, occurring more than 4 hours after I.V. injection of metampicillin.

Ampicillin↗

PubMed1977

Pseudo-clinical Fabry's disease without alpha galactosidase deficiency.

The authors describe two cases of clinical Fabry's disease. The first patient presents a deficiency of alpha galactosidase and a urinary excretion of ceramide trihexosides and dihexosides ; the second patient had a normal alpha galactosidase and normal excretion of urinary lipids. In this latter case the Km and the activity of the enzyme measured at different pH were similar to those of normal enzyme. The other lysosomal enzymes, beta galactosidase, beta glucosidase, hexosaminidases A and B, alpha fucosidase, arylsulfatases, phosphatase acids were also measured in patient 2 and all have normal activities. There is no urinary excretion of glycolipids or mucopolysaccharides. Yet this patient has an accumulation of material in his fibroblasts and renal cells. The authors also present a genetic study.

Adult↗

PubMed1977

[Treatment of amenorrhea-galactorrhea with bromo-ergocryptine. Apropos of 12 cases].

The authors report the action of bromergocryptine in 12 patients who were suffering from a syndrome of amenorrhoea with galactorrhoea and hyperprolactinism. None of these 12 patients had a pituitary tumour; or else they had been operated on before treatment. Only in one case was the syndrome linked to taking oral contraceptives, which had been stopped more than six months previously. The dose of bromergocryptine was 5 mg. a day. If this dose fails it is possible to raise it after two months' observation. The galactorrhoea disappeared or markedly decreased in 8 out of the 12 cases. Ovulatory cycles started in 9 out of the 12. 5 of the women wanted a pregnancy: 4 succeeded and 2 of them have already been delivered of normal children. Bromergocryptine (CB 154) has thus proved itself to be remarkably effective in hyperprolactinism, not caused by a pituitary tumour, which gives rise to amenorrhoea and galactorrhoea. All the same, its long-term use, especially after stopping treatment, is still to be evaluated.

Adult↗

PubMed1976

[Prevention of stress ulcer by vitamin A. Experimental study in rats].

71 male rats (Whitstar strain) are submitted to restraint. 37 of them were receiving twice an injection of 10 000 I.U. of vitamin A in order to protect them against the occurence of stress ulcer. Untreated animals are used as controls. Incidence of obtained ulcer is compared with the variation of vitamin A serum concentration, classicaly decreased in stress. Analysis of individual results shows that, if parenteral administration was followed either by restitution of the initial serum concentration, or by its increase, the treatment was efficient in 64 p. 100 of the cases.

Animals↗

PubMed1976

[Induction of N-acetyllactosamine synthetase activity in the mouse mammary gland by prolactin and placental lactogen hormone].

The authors show that the ovine prolactine promote induction of N. acetyl lactosamine synthetase in tissue culture of mammary glands of pregnant mice. A crude extract of human placenta has also a lactogenic activity as tested by the same method, but in this case the blank values are very high for large concentration of crude extract. The molecular forms of HCS are tested: the slow band has a lactogenic activity, the intermediate band has no activity and the rapid band seems to be inhibitory.

Animals↗

PubMed1976

[Molecular forms of human growth hormone (HGH) secreted by cultured fetal pituitary cells].

Cultures of foetal human pituitary cells excrete and synthetize different molecular forms of proteins with HGH immunological activity. These cells incorporate tritiated-leucine in these proteins. Gel chromatography on sephadex using different length of column allow us to separate: One form excluded in front of the dead volume and which has a high molecular weight. This form is not dissociated by treatment with urea 8 M, guanidine 6 M and dithiothreitol. Nor this form is modified by treatment by ribonuclease. One form excluded within the dead volume and which is probably a dimere. This form is no more modified by the different treatments. One form which is excluded like a monomere--it is the more important form--. This form is dissociated in fragments of lower molecular weight by urea 8 M. This dissociation is partially reversible by dialysis.

Cells, Cultured↗

PubMed1976

[Heterogeneity of ovine prolactin after labeling with iodine 125: value of labeling with lactoperoxidase].

The authors have shown an heterogeneity of the ovine prolactine molecule after labelling with iodine 125. As well with chloramine T as with lactoperoxydase, it appears three molecular species which react with the immune serum antiprolactine (I.S.). The first species is of high molecular weight and is probably constituted of aggregates. Their combination with the I.S. is non specific and give blanks of high value. The second species is a dimere and the third one is the monomere. The two last species react with the I.S. and can give competition curves when they are choosen as tracer. However, if one uses as tracer a product obtained by labelling with chloramine T, the competition appears for high concentrations of native hormone. As if the I.S. recognizes much more the labelled protein than the native one. But if one uses the same species but labelled with lactoperoxydase, the competition appears for concentrations lower than five nanogrammes. In the same time one can see that the specificity toward different I.S. is modified. The authors think that the labelling with lactoperoxydase better preserves the tertiary structure of the native protein than do the labelling with chloramine T.

Animals↗

PubMed1976

[Iso-hormones : molecular forms of human chorionic somatomammotropin (HCS) (author's transl)].

The chorionic somatomammotropin hormone extracted from the human placenta exists in several molecular forms: Analytical electrophoresis on polyacrylamide gel permits separation of a highly anodic migration form : form 1 and another form migrating slightly faster than albumin : form 2. These two forms are active as measured by radioactive immunological analysis, form 2 being about 25 times more active than form 1. The two forms are mutually interconvertible. The two forms may also be separated by filtration on Sephadex G-50. However, they do not differ in molecular weight, they have the same coefficient of apparent diffusion, measured by analytic ultracentrifugation. Glutaraldehyde and 8 M urea do not modify their electrophoretic or chromatographic behaviour. On the other hand, the two forms differ in their tertiary structure, with the modification depending on the greater or lesser degree of oxidation of the intra-chain disulfide groups. The two forms also exist in placental culture media and the incorporation of tritiated leucine occurs preferably in form 1, The physiological significance of the two hormone pools is not clarified.

Chromatography, Gel↗

PubMed1975

Isohormones: molecular forms of the human chorionic somatomammotropic hormone.

The chorionic somatommaotropic hormone extracted from the human placenta exists in several molecular forms. Analytical electrophoresis in polyacrylamide gel permits separation of a highly anodic migration form, form 1, and another form migrating slightly faster than albumin, form 2. These two forms are active as measured by radioactive immunological analysis, form 2 being about 25 times more active than form 1. The two forms are mutually interconvertible. The two forms may also be separated by filtration on Sephadex G-50. However, they do not differ in molecular weight, they have the same coefficient of sedimentation and the same coefficient of apparent diffusion, measured by analytic ultracentrifugation. Glutaraldehyde and 8 M urea do not modify their electrophoretic or chromatographic behavior. On the hand, the two forms differ in their tertiary structure, with the modification depending on the greater or lesser degree of oxidation of the intra-chain disulfide groups. The two forms also exist in placental culture media and the incorporation of tritiated leucine occurs preferably in form 1. The physiological significance of the two hormone pools is not clarified.

Animals↗

PubMed1974