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Biomedical subjects

F Bischof

Publications and source records attributed to F Bischof.

32 records · Page 2Linked to original sources
PubMed1994

Unaltered respiratory chain enzyme activity and mitochondrial DNA in skeletal muscle from patients with idiopathic Parkinson's syndrome.

There is good evidence that patients with Parkinson's disease have respiratory chain dysfunction in their substantia nigra. Since mitochondrial cytopathies due to enzyme defects in the respiratory chain are predominantly manifested in tissues with a high oxidative metabolism we analyzed oxidative energy metabolism in skeletal muscle from 6 patients with Parkinson's disease. Control muscles were from subjects of the same age group. Histological and histochemical analyses showed no morphological abnormalities found in mitochondrial myopathies. Biochemical analyses of the various complexes of the respiratory chain were normal. Since 13 subunits of complexes I, III, IV and V of the respiratory chain are encoded by the mitochondrial genome we performed Southern blot and PCR analyses in skeletal muscle from patients and controls and found no disease-specific increase in deletions or insertions of the mitochondrial genome. Therefore, we do not think that skeletal muscle reflects the mitochondrial disturbance in Parkinson's disease found in the substantia nigra.

Aged↗
PubMed1994

Multiple symmetric lipomatosis: abnormalities in complex IV and multiple deletions in mitochondrial DNA.

Multiple symmetric lipomatosis (MSL) is a rare disorder of middle life characterized by large nonencapsulated lipomas distributed around the neck, shoulders, and other axial regions. Neurologic involvement, particularly peripheral neuropathy, is frequent. The pathogenesis of the syndrome is still unknown, but ragged-red fibers are occasionally present in muscle of affected patients, suggesting a mitochondrial abnormality. We studied 11 unrelated patients with MSL by means of neurophysiology, muscle morphology, muscle biochemistry, Southern blot, and PCR analysis of mitochondrial DNA. All patients were men aged 41 to 63 years. Clinical or electrophysiologic signs of a sensorimotor polyneuropathy were present in nine patients, eight of whom had a history of alcoholism. In muscle biopsy specimens, the most prominent feature was pathologic subsarcolemmal aggregates of mitochondria. Biochemical analysis of respiratory chain enzymes revealed a moderate but significant decrease of cytochrome c oxidase activity as compared with age-matched controls. In one patient, Southern blot analysis showed multiple deletions of mitochondrial DNA. We conclude that mitochondrial dysfunction is common in MSL and may be based on identifiable defects in the mitochondrial genome.

Adult↗
PubMed1993

On line continuous monitoring of blood lactate in men by a wearable device based upon an enzymatic amperometric lactate sensor.

A wearable device for the continuous measurement of lactate in the blood was constructed by the combination of continuous blood sampling employing a double lumen catheter with an amperometric lactate sensor. In vitro, the lactate sensor turned out to have a linear concentration range between 0 and 15 mmol/l. The response time of the sensor itself amounted to 100 sec, whereas the lag time for blood sampling amounted to 2.2 min. In vivo, the lactate sensor was successfully used for the detection of changes of the blood lactate concentration following strenuous exercise in 7 healthy volunteers, in two cases up to 22 h. In conclusion, the technique of continuous blood sampling by the use of a double lumen catheter in combination with the amperometric lactate sensor is feasible and simplifies frequent blood lactate estimations.

Adolescent↗
PubMed1993

On line continuous monitoring of subcutaneous tissue glucose is feasible by combining portable glucosensor with microdialysis.

A new method for continuous measurement of subcutaneous tissue glucose content is introduced: by combining the microdialysis technique with a wearable amperometric glucose sensor, a device for continuous glucose measurement in the subcutaneous tissue was obtained. This device was applied to healthy volunteers (n = 10) over the period of an oral glucose load and to type I diabetic patients (n = 10) under the conditions of daily life. Glucose profiles in both healthy and diabetic persons were followed in the subcutaneous tissue up to 27 hours. This technique will certainly open new perspectives of monitoring and treating diabetic patients.

Biosensing Techniques↗
PubMed1993

Use of the microdialysis technique in the monitoring of subcutaneous tissue glucose concentration.

For some time the subcutaneous (s.c.) tissue has been the target for continuous glucose measurement. The microdialysis technique permits an extracellular region approach, which has been used for about two decades for measuring various metabolites in dialysates obtained from different body regions. By connecting a s.c. implanted microdialysis probe to a flow chamber of an amperometric glucose sensor, the procedure of glucose sensing was transferred to ex vivo. Using this device it was possible to obtain, for up to 24 hours, s.c. tissue glucose profiles of healthy and diabetic people. The microdialysis theory, the calibration process and other microdialysis technique applications are discussed in this paper. Although the combination of the microdialysis technique and amperometric glucose sensing requires certain technical equipment, the combination of microdialysis and glucose sensor seems to be a promising approach to a continuously functioning glucose sensing system.

Blood Glucose↗
PubMed1992

On line continuous monitoring of subcutaneous tissue glucose in men by combining portable glucosensor with microdialysis.

For the normalisation of blood glucose levels in diabetic patients by feedback controlled insulin delivery, a self-manageable and reliable method for continuous glucose estimation is still not available. By combining a commercially available needle type dialysis probe (molecular cutoff 20,000 Da) with a sensitive glucose sensor, we obtained a device for continuous glucose measurement in dialysate. This device was tested in healthy volunteers during a 75-g oral glucose tolerance test and in Type 2 (non-insulin-dependent) diabetic patients. Venous glucose and subcutaneous sensor signal were followed for 300 min (ten healthy subjects), 21 h (three healthy subjects) or 9 h (seven Type 2 diabetic patients). The recovery of the microdialysis was interindividually different, but after calibration, glucose levels in the dialysate and subcutaneous glucose sensor signal correlated well (r = 0.84-0.95). Under the assumption of a physiologic and technical delay between intravenous and subcutaneous glucose, correlation coefficient between intravenous glucose and subcutaneous sensor signal ranged from 0.60 to 0.93. We conclude that changes in blood glucose could be monitored in the subcutaneous tissue by the microdialysis technique in a continuous on line manner.

Adult↗
PubMed1992

Experiences with a computerized diabetes information system.

This study shows the results of working with a computerized system which stores values of blood glucose, insulin, diet and exercise and demonstrates which diabetic patients are suitable for continuous monitoring. The recording of blood glucose as well as the insulin doses, diet and exercise correlated to time is more accurate and easier than the usual protocolling of data. Deficiencies in diet, exercise or insulin regimens are detected more easily by evaluation of data with a computerized system, for example by making an average profile of a day. These results have a strengthening effect: Well controlled diabetic patients are ambitious to further improve their blood glucose control. Poorly controlled diabetics appear to be frustrated by this kind of unfailing control.

Adolescent↗
PubMed1988

Effects of celiprolol on serum lipids in systemic hypertension.

Antihypertensive therapy with celiprolol lowers blood pressure by selectively blocking beta 1 adrenoceptors. It also exhibits vasodilatory and bronchosparing effects and is not cardiodepressive. Because many beta blockers are often suspected of adversely raising serum lipid levels, especially triglycerides, a special investigation of lipids in hypertensive subjects was performed. The preliminary findings in a series of 14 patients (average age 39.7 years) with essential hypertension (World Health Organization stages I to II) who were treated with celiprolol for a period of 6 months are presented. Serum levels of total cholesterol and low-density lipoprotein remained virtually unchanged during treatment. There was, however, a tendency for triglyceride levels to fall with celiprolol treatment, a trend that became significant after 4 weeks. Moreover, high-density lipoproteins tended to increase with treatment and were significantly increased after 2 weeks. In contrast to findings obtained with other beta-blocking agents, no increases in total serum lipids were observed during celiprolol treatment. Blood pressure values of the subjects treated decreased significantly from a mean value (measured in the upright position) of 151/99 to 131/87 mm Hg. Blood pressure values taken in the supine position were also significantly reduced. No significant changes were noted in a variety of laboratory parameters, including blood count, blood coagulation time, blood sugar concentration, liver and kidney tests, electrolyte levels and urinalysis. The significance of these results, which suggest that celiprolol is not associated with adverse changes of lipids and may even positively influence blood chemistry, is discussed.

Adult↗
PubMed1988

Reduction of left ventricular hypertrophy in hypertensive patients after treatment with celiprolol.

Long-term treatment with celiprolol, a new cardioselective beta blocker, significantly reduced blood pressure in 14 patients with essential hypertension (World Health Organization stages I to II). Heart rates remained within the normal range throughout treatment and no significant side effects were observed. In 4 patients, end-systolic septum thickness was measured, and evidence was obtained that the extent of left ventricular hypertrophy was reduced. These findings indicate that celiprolol can be used for the successful long-term treatment of essential hypertension, and that such treatment reduces left ventricular hypertrophy induced by high blood pressure.

Adult↗
PubMedDate not supplied

Arthroscopic labrum refixation for post-traumatic anterior shoulder instability: suture anchor versus transglenoid fixation technique.

SUMMARY: The aim of this retrospective study was to compare recurrence rates following transglenoid labrum refixation or fixation using the suture anchor (FASTak, Arthrex, Naples, FL) technique. Additionally, parameters that apparently influence the rate of redislocation were investigated. There were 163 patients with post-traumatic anterior shoulder instability treated with an arthroscopic labrum refixation; 108 patients (66.3%) were stabilized with the transglenoid suture technique (group I) and 55 patients (33. 7%) with the suture anchor (FASTak) technique (group II). The average follow-up was 4.5 years (range, 2.0 to 7.9 years) in group I and 3.2 years (range, 2.0 to 5.0 years) in group II. The Rowe score increased from a preoperative average of 35.0 points in group I and 35.4 points in group II to a postoperative average of 68.3 points in group I and 84.6 points in group II (P <.01). There was recurrence in 35 patients (32.4%) in group I and 9 patients (16.4%) in group II (P <.05). All incidents of redislocation occurred during the first 21 postoperative months; 58.4% of the patients (n = 63) in group I and 16.4% of the patients in group II (n = 9) had to reduce their sporting activity (P <.001). Independent of the type of surgery, there was a significant correlation of the postoperative rate of redislocation and age (P <.001), number of preoperative dislocations (P <.01), and degree of labrum lesion (P <.001). No correlation with the rate of redislocation was shown for gender, handedness, dislocation-operation interval, degree of Hill-Sachs lesion, or number of transglenoid sutures or anchors. Concerning post-traumatic anterior shoulder instability, the arthroscopic labrum reconstruction with the suture anchor (FASTak) technique was superior to the transglenoid technique but has not yet achieved the level of success obtained by open surgery. With fewer than 5 preoperative redislocations after a first traumatic shoulder dislocation, the arthroscopic treatment is recommended. In cases of more frequent preoperative dislocations, open surgery in combination with a capsular shift should be performed.

Adult↗
PubMedDate not supplied

[Individual cement-free total hip endoprosthesis in a patient with a rare form of dwarfism (Fuhrmann syndrome)].

We present a single case study of a 47-year-old female patient with a rare form of primary dysostotic dwarfism. This syndrome was first described by Fuhrmann in 1972. Seven years ago, this patient underwent bilateral cementless total hip arthroplasties for severe osteoarthritis about her hips. Custom-made components were used. While achondroplastic patients present with wide femoral medullary canals, it is clinically relevant that Fuhrmann Syndrome features narrow femoral medullary canals. This complicating anatomical factor in Fuhrmann syndrome as well as the increased anteversion angles were overcome by the use of CAD stems. We conclude that the custom-made stems, although in our case measuring half the size of the smallest commercially available design, have proved to be durable and able to withstand physiological loads at follow-up period of 72 months.

Arthroplasty, Replacement, Hip↗
PubMedDate not supplied

[Arthroscopic labral reconstruction for anterior shoulder instability. Failure analysis in 187 patients].

UNLABELLED: Comparing open with arthroscopic labral reconstruction for anterior shoulder instability we found a higher recurrence rate after arthroscopic capsulolabral repair. The aim of this study was, to analyse the reasons for recurrent instability after arthroscopic labral repair. MATERIALS AND METHODS: Between 1989 and 1995 we performed a arthroscopic labral reconstruction on 187 patients with anterior shoulder instability. 118 patients (63%) were treated with a transglenoid suture technique and 69 patient (37%) with a suture anchor technique. The average age at the time of the operation was 26.4 +/- 6.3 years (14-52 years). There were 41 women (21.9%) and 146 men (78.1%). RESULTS: The minimum follow-up was 18 months. The average follow-up was 3.7 +/- 1.1 years. The average Rowe Score increased from 34.7 points (0-75) preoperatively to 75.3 points (15-100) postoperatively. There were 47 (25.1%) excellent. 76 (40.6%) good, 21 (11.3%) fair, and 43 (23.0%) poor results. 105 patients (56.1%) regained their preoperative level of activity. 50 patients (26.7%) had recurrent subluxations or dislocations postoperatively. All failures occurred within two years (0.5-21 months) after the operation. Failure rates were associated with the patients age (p < 0.001), the level of activity (p < 0.05), the number of the preoperative dislocations (p < 0.01), the degree of the labral lesion (p < 0.001), and the operation method (p < 0.05). No correlation was found for the parameters sex, handiness, time between luxation and operation, size of Hill-Sachs-lesion and numbers of used sutures or anchors. CONCLUSION: We prefer the arthroscopic suture anchor technique if there are less than 5 preoperative dislocation and a type 1 or 2 labral lesion. If there are more than 5 preoperative dislocations and a degenerative labrum defect we favor the open Bankart repair technique.

Adolescent↗
PubMedDate not supplied

Cerebral vein thrombosis in a case with thromboangiitis obliterans.

Thromboangiitis obliterans is a chronic inflammatory vessel disease that involves predominantly the small and medium-sized arteries and veins of the distal extremities. Appearance and cessation of symptoms are closely related to patterns of tobacco consumption. That cerebral arteries can also be involved is shown by reports of rare cases in which cerebral artery occlusion led to infarction. We report on a 28-year-old man with thromboangiitis obliterans who developed extensive cerebral vein thrombosis after a single episode of cigarette smoking following several years of nonsmoking. Despite extensive evaluation, no other known cause or predisposition of cerebral vein thrombosis could be found. This case suggests that cerebral veins can be involved in thromboangiitis obliterans and patients with thromboangiitis obliterans might be at risk for cerebral vein thrombosis.

Adult↗