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Biomedical subjects

F Blanco

Publications and source records attributed to F Blanco.

46 records · Page 3Linked to original sources
PubMed1985

[Fractures of the long metacarpals. Long-term results].

Sixty-seven of long metacarpal fractures have been followed for more than one year. Digital mobility and strength of grasp of the traumatized hand were measured and then compared with the healthy hand, the result being modified, according to the patient's own appreciation. This clinical examination enabled a medium and long term assessment of functional results. Control radiograms helped us draw up a chart of the various types of fractures, their topographical distribution and eventual anatomical modifications according to displacement. When comparing clinical and radiographic results, a correlation is--or is not--possible between the functional deficit and the degree of deformation of the metacarpals. Clinical results also depend on other factors which may influence the final result.

Adult↗
PubMedDate not supplied

Inelastic scattering and stopping power of low-energy electrons (0.01-10 keV) in toluene.

In this study, the stopping power for electrons in toluene is reported for incident energies ranging from 10 to 10,000 eV. The present results have been obtained by combining the calculated inelastic electron scattering cross-sections with an experimental energy loss procedure. Calculations have been carried out by means of a quasifree absorption model whose reliability has been checked by comparison with empirical electron scattering total cross section data. Results have been compared with the high-energy stopping power data available in the literature. For energies below 1 keV these are the first results of the stopping power for electrons in toluene.

Algorithms↗
PubMedDate not supplied

Identification of a 35 kDa glycoprotein from Entamoeba histolytica by sera from patients with amoebic liver abscess and with mouse monoclonal antibody.

In this work, we explored the relevance of a 35 kDa glycoprotein (Gm) of the outer membrane from E. histolytica in the diagnosis of the amoebic liver abscess (ALA) through ELISA and immunoblotting. We were interested in defining the relevance of this antigen in the immune response in patients with amoebic liver abscess and in exploring whether the mouse monoclonal antibody against this 35 kDa glycoprotein recognises the same epitope. We found that 87% of ALA patients had raised antibody levels to Gm antigen, whereas none of the healthy control subjects presented this same increase. We also found 90% sensitivity, 100% specificity, 100% positive predictive value, 90% negative predictive value, and 90% prevalence value for this Gm antigen. Nonetheless, we did not find any statistically significant differences in the levels of immunoglobulins against Gm, although IgG showed a tendency to increase, probably because we are dealing with a secondary immune response. Using electroimmunotransfer blot assay, we found that sera from ALA patients recognise the 35 kDa Gm protein in the same way as it is recognised by the mouse monoclonal antibody, suggesting that is a relevant molecule for the diagnosis of amebiasis, and eventually could lead to its use as protection against the disease.

Animals↗
PubMedDate not supplied

[Elaboration of an immunosorbent for the purification of porins from Salmonella typhi 9, 12, Vi:d].

The current work was undertaken to purify porins of Salmonella typhi, which are outer membrane proteins (OMPs) that induce protection in mice against challenge with the bacteria in mucin. OMPs, isolated with a non-ionic detergent, had a 4% contamination with LPS (endotoxin) and molecular sizes ranging from 17 to 70 KDa. Porins (Mw 38-41 KDa) were isolated from OMPs preparative SDS-PAGE. Anti-porins antisera were raised in rabbits and specific IgG was purified, which was coupled to Sepharose-CNBr. This immunosorbent was used to purify LPS-free porins.

Antibodies, Bacterial↗
PubMedDate not supplied

[Risk factors in laparoscopic cholecystectomy].

In this report the authors have analysed the various risk factors of the laparoscopy cholecystectomy, proving that those are not different from the ones of standard open cholecystectomy. It has been underlined that laparoscopy cholecystectomy offers significant advantages over open cholecystectomy and moreover the possibility to convert laparoscopy cholecystectomy when it is necessary.

Age Factors↗
PubMedDate not supplied

[Follow up in carcinoma of the large intestine].

A valid program of follow-up has always been a crucial point in the overall therapy of the colon-cancer. In this retrospective study, the authors have used as specimen 74 patients put under observation between the years 1987 and 1992. The patient have been followed throughout the diagnostic period with various methods. It has been the will of the authors, who have presented their protocol of reference, to put under comparison the various controlling methods in order to visualize their reliability, specificity and the indication of each one of them. The CEA is the most sensible haemanalysis for lifting the doubt of recidivation. As for the TAC and ultrasound it has been reserved the job of formulating a correct diagnosis; the results of both diagnostics through imagery have been more or less the same. However, the ultrasound examination have shown more false positives than the TAC. The research of the blood occult in the stool is a rapid and economic detection in the case of intramural recidivations, even if we cannot disregard the share of false positives. A high specificity for the study of intramural recidivations has been offered by the endoscopic scan particularly when associated by a brushing and biopsy.

CA-19-9 Antigen↗
PubMedDate not supplied

[Emergencies in oncologic surgery of the large intestine].

The authors analyse of the large intestine at their Institute over the past 20 years. Four hundred and fifty-two cases out of 842 were performed during the period 1970-1979 and 300 during the subsequent decade. 21% (95 cases) were emergencies during the first decade and 16% (62 cases) during the second. From this study it emerges that patients operated with primary resection during the first decade 1970-1979 had a better survival rate than those operated in various stages. This was particularly true of cases of occlusion, whereas in cases of perforation the percentage remained practically unchanged. The approach adopted for this type of pathology changed during the next decade and it was decided to opt for primary resective surgery. A comparison between emergency surgery performed in a single session and operations performed in a number of stages revealed that both survival and morbidity were improved in primary resective surgery, 20% and 10% respectively, whereas mortality was higher (15%).

Emergencies↗