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F Boehlen

Publications and source records attributed to F Boehlen.

24 records · Page 2Linked to original sources
PubMed1998

Evaluation of a new antigen capture ELISA for detection and characterization of platelet alloantibodies.

The monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay is considered as a possible reference method for the detection of maternal alloantibodies when a foetomaternal alloimmunization is suspected. However, this method is tedious. In this study, we have compared the MAIPA results of 54 samples of mothers with (n=34) or without (n=20) alloantibodies with those obtained with a new antigen capture ELISA. Using the cutoff of 2.0 given by the manufacturer, the new kit had a sensitivity of 88.2% (95% CI 72.6-96.7) and a specificity of 100% (95% CI 98.0-100). From a receiver-operating characteristic curve analysis, the more appropriate cutoff for a screening assay would be 1.6, which gives a sensitivity of 100% (95% CI 89.7-100) with a specificity of 94.0% (95% CI 89.4-96.9). In conclusion, this new simple assay appears promising and could be used, with the modified cutoff, as a screening assay for the detection of platelet alloantibodies.

Antibodies, Monoclonal↗
PubMed1998

Neonatal thrombocytopenia: incidence and characterization of maternal antiplatelet antibodies by MAIPA assay.

Neonatal thrombocytopenia (NNT) which is frequent in distressed newborns was uncommon in a non-selected population of neonates. The aim of this prospective study was to determine the frequency of NNT and, in confirmed NNT, to search for maternal antiplatelet antibodies with a monoclonal antibody-specific immobilization of platelet antigens (MAIPA) assay. Among the 8388 newborns studied, 40 (0.5%, 95% CI 0.3-0.6) had confirmed NNT, which was severe (platelet count < 50 x 10[9]/l) in 10 cases (0.12%, 95% CI 0.05-0.20). Antiplatelet antibodies were detected in 10/31 studied mothers of thrombocytopenic newborns (32.3%): they were alloantibodies in five cases and autoantibodies in five other cases. Among these 10 newborns, seven had severe thrombocytopenia and four had bleeding complications. As controls, antiplatelet antibodies were also searched for in mothers of non-thrombocytopenic newborns: antiplatelet antibodies were present in 8.5% (95% CI 5.9-11.7) of thrombocytopenic mothers (n = 400) and 3.2% (95% CI 0.7-9.0) of non-thrombocytopenic mothers (n = 95). The difference was significant between the control groups and the group of mothers of thrombocytopenic newborns. In conclusion, our data indicate that an immune origin is frequent in NNT and should be looked for, particularly when the platelet count is < 50 x 10(9)/l.

Antibodies, Monoclonal↗
PubMed1998

Severe neonatal alloimmune thrombocytopenia due to anti-HPA-3a.

BACKGROUND AND OBJECTIVES: Neonatal alloimmune thrombocytopenia is usually attributable to HPA-la antibodies. We report a case of parental platelet antigen incompatibility associated with a severe neonatal thrombocytopenia secondary to alloimmunization to HPA-3a. MATERIALS AND METHODS: Platelet antibodies were detected by the monoclonal antibody-specific immobilization of platelet antigens, genotyping of the platelets by PCR, and HLA typing by serologic procedures and PCR. RESULTS: Genotyping of maternal and paternal platelets confirmed the incompatibility in the HPA-3a system. It is noteworthy that the mother is of the HLA type DRB3*0101, is ABO-incompatible with her husband, and also has HLA class I antibodies. CONCLUSION: Severe neonatal thrombocytopenia associated with HPA-3a alloimmunization is infrequent and all the factors mentioned above could have played a role in the severity of the disease.

Adult↗