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F Bonhoeffer

Publications and source records attributed to F Bonhoeffer.

63 records · Page 4Linked to original sources
PubMed1972

Replication of Phi-X174 DNA by Escherichia coli polA- in vitro (Phi-X174 DNA-DNA replication-E. coli polA-).

Lysates of an Escherichia coli polA(-) strain convert single-stranded DNA from varphiX174 virus to the double-stranded replicative form with high efficiency on cellophane discs. The initiation of synthesis of the complementary strand appears to be rate limiting; once initiation occurs, the chain is propagated rapidly. Under these conditions, the unsealed replicative form accumulates and is slowly converted to the sealed form; this conversion requires the activity of the DPN-dependent DNA ligase.

Centrifugation, Density Gradient↗
PubMed1972

DNA replication.

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Adenosine Triphosphate↗
PubMedDate not supplied

Inhibition of axonal growth by a monoclonal antibody.

Little is known about factors which control the outgrowth of neurites in the central nervous system (CNS) during development, although in vitro studies with neurones from the peripheral nervous system have shown that neurites require an appropriate substrate and specific factors for growth. To investigate the role of cell-surface components in the development of the CNS, we have raised a series of monoclonal antibodies against membrane components of cells from the visual system of the chick embryo. We report here that one of these antibodies, T61/3/12, clearly inhibits axonal outgrowth from chick retinal explants but does not affect axon growth from the peripheral nervous system.

Animals↗
PubMedDate not supplied

Position-dependent properties of retinal axons and their growth cones.

The formation of the very orderly neuronal projection from the retina to the optic tectum is not yet understood, but several mechanisms are thought to be involved in a coordinated fashion. These mechanisms may include mechanical or chemical guidance in channels, guidance by spatial gradients of positional markers, gradients of temporal (maturation) markers or specific inter-axon interactions (see ref. 1 for review). The last-mentioned mechanism could explain the fibre order found in optic nerve and tract. It requires that some or all growing retinal axons can distinguish between retinal axons of various origins and grow preferentially along retinal axons originating from the same area as themselves. The in vitro experiments described here show that growth cones from the temporal half of the chick retina grow preferentially along temporal axons, whereas growth cones from nasal retina do not distinguish between nasal and temporal axons.

Animals↗