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Biomedical subjects

F Busolo

Publications and source records attributed to F Busolo.

28 records · Page 2Linked to original sources
PubMed1981

Survival of genital mycoplasma on various bacteriological swabs and transport media.

An approach to diagnostic microbiology necessitates measures which maintain the flora unchanged during the transportation of the specimens to the laboratory, preserving the viability of labile and fastidious organisms such as mycoplasma. Some of these problems have been studied in the present investigation. Five different swabs (untreated, albu, charcoal, alginate and phosphate-buffered albumin) and three transport media (Stuart, Amies and MTB) were examined using genital mycoplasma. Absorbent effect of swabs treated with albumin is lower than all the others while the antimycoplasma activity, due to the toxic substances present in the fiber of tip swab, is always lower for the charcoal swab. Concerning transport media, the most favourable results were obtained with the MTB medium. In this medium M. hominis and U. urealyticum counts decreased about 0.5 log and M. fermentans decreased 1.25 log after seven days at 4 degrees C. Furthermore, after inoculation of mycoplasma, MTB can be frozen at -20 degrees C without greatly modifying the titer. The reasons of the different performance of transport media on mycoplasma survival were discussed.

Absorption↗
PubMed1980

Colony morphology, ultrastructure and morphogenesis in Mycoplasma hominis, Acholeplasma laidlawii and Ureaplasma urealyticum.

Colonies of Mycoplasma hominis, Acholeplasma laidlawii (three strains) and Ureaplasma urealyticum were examined by light and electron microscopy and their characteristic morphology, ultrastructure and morphogenesis are described. Mycoplasma hominis and A. laidlawii, PG8 and oral strains, developed typical 'fried-egg' colonies which were remarkably heterogeneous in size. The colonies of A. laidlawii strain NCTC 10116 were more homogeneous and grew mainly on the surface of the agar showing a fine granular appearance. Ureaplasma urealyticum produced smaller, granular colonies which grew deeply embedded in the agar and generally without much surface growth. The cellular ultrastructure in these colonies was also examined. The results indicate that several aspects of colony morphogenesis and ultrastructure varied for each of the three species examined.

Acholeplasma laidlawii↗
PubMed1980

Enzyme-linked immunosorbent assay for detection of Mycoplasma pneumoniae antibodies.

The enzyme-linked immunosorbent assay (ELISA) described by Engvall and Perlmann has been used for the detection of antibodies against Mycoplasma pneumoniae. These organisms, grown in flat-bottom wells of microtiter plates, were used as antigen. Preliminary results suggest that ELISA is specific and that its sensitivity is somewhere in between those of the metabolic inhibition and radioimmunoprecipitation methods. Unlike other serological methods, such as metabolic inhibition and mycoplasmacidal tests, in which the presence of antibiotics in sera may give false results, ELISA provided results that were not influenced by antibiotics. Furthermore, ELISA offers the possiblity of measuring not only immunoglobulin M, but also complement-independent antibodies, especially those of the immunoglobulin A class. Theoretical aspects concerning the different sensitivities of some serological reactions are discussed.

Antibodies, Bacterial↗
PubMed1979

Comparative evaluation of some methods for detection of M. pneumoniae antibodies.

Various methods of detection are available for measuring antibodies specific to M. pneumoniae. We examined over 600 sera of patients with clinical diagnosis of M. pneumoniae infection using complement fixation (CF) test. 91 gave positive reaction and were therefore selected for a comparative analysis with other methods such as metabolic inhibition (MI), mycoplasmocidal (MC) and radioimmunoprecipitation (RIP) methods. MC and RIP test gave antibody titers comparatively higher than MI and CF. Comparative studies on the sensitivity of the various methods considered were also carried out on 6 paired sera collected from the same patients at 10 days intervals. The increase in antibody titers were much higher when MC and RIP tests were considered. When RIP and MC methods were compared, the first appeared more sensitive in all cases studied.

Animals↗
PubMed1979

In vitro antimycoplasmal activity of mepartricin.

The in vitro antimycoplasmal activity of mepartricin was evaluated on several mycoplasma strains. The results demonstrate that this polyene antibiotic possesses a high efficacy against these microorganisms.

Acholeplasma laidlawii↗
PubMed1975

[Induction of prophage gamma by means of bleomycin treatment].

The kinetics of induction of lambda prophage with bleomycin A2-CP3, mitomycin C and a combination of these two antibiotics have been studied. Bleomycin has been found to cause a rapid induction of prophage in exponentially growing cultures of E. coli K12 (lambda), and the maximal values of including activity are obtained after treatment for 16 minutes with a concentration of 10 mug/ml. The per cent of induction, calculated from the total number of surviving cells, including the phage producing cells, better expresses the dynamics of the phenomenon. The number of inducing events in the population of exposed cells is proportional to the length of bleomycin treatment. These values are greater than those observed after treatment with mitomycin, at the same concentration. Inducing activity is enhanced by combination of the two antibiotics and this effect is not additive, but synergic. After the 16 minutes of treatment with bleomycin the burst sizes are lower than those which are obtained with mitomycin or with the combination of the two antibiotics. The results are interpreted as a consequence and a confirmation of the different mechanisms of action that distinguishes bleomycin from mitomycin.

Bleomycin↗
PubMedDate not supplied

Microbial flora in semen of asymptomatic infertile men.

A microbiological examination has been carried out in 116 patients with unexplained infertility and with asymptomatic bacteriospermia. Organisms more frequently isolated were Staphylococcus epidermidis (81.9%), non hemolytic streptococci (23.3%), diphtheroids (25%) and alpha-hemolytic streptococci (18.1%). None of the 103 patients were positive for C. trachomatis. Mycoplasmas were isolated in 56 (48.3%) of the 116 examined samples, U. urealyticum was present in 49 (42.2%), M. hominis in 3 (2.6%) and both species in 4 (3.5%) samples of examined fluids. Forty-one of the 56 mycoplasmas-positive patients have been treated with doxycycline therapy. The treatment improved motility and caused decrease of coiled tails in 12 cases. Conception occurred in 5 (26.3%) of the 19 treated patients whose cultures were negative for mycoplasmas. These 5 pregnancies occurred in the patients that had high titers (greater than 10(5) c.c.u./ml) of ureaplasmas and in which the antibiotic therapy was successful. No pregnancy was seen in the other 22 patients where treatment failed to eradicate mycoplasmas.

Chlamydia trachomatis↗
PubMedDate not supplied

Serodiagnosis of M. pneumoniae infections by enzyme-linked immunosorbent assay (ELISA).

The enzyme-linked immunosorbent assay (ELISA) has been used for detection of antibodies against Mycoplasma pneumoniae. The results of ELISA and its sensitivity compared with other serological methods, such as complement fixation (CF), metabolic inhibition (MI), mycoplasmacidal test (MC), and radioimmunoprecipitation (RIP) are reported. ELISA and MC showed greater sensitivity than CF and MI, while RIP showed serum titer two- to 16-fold higher. ELISA was specific as determined using other human mycoplasma. A simplified method based on the determination of ELISA antibody end-point titer by a single serum dilution has been proposed. ELISA presented several advantages: sensitivity, rapidity, and low cost and, if adequately standardized, could become a reliable method for the serodiagnosis of M. pneumoniae infection.

Antibodies, Bacterial↗