PubMed Health⌕ Search

Biomedical subjects

F C Ballantyne

Publications and source records attributed to F C Ballantyne.

At least 37 records · Page 2Linked to original sources
PubMed1980

Comparison of immunonephelometry and electroimmunoassay for estimation of plasma apolipoprotein A-I.

Immunonephelometry using a helium-neon laser as light-sources has been compared with the established technique of electroimmunoassay for estimation of plasma apolipoprotein A-I (apoA-I), the major protein component of high density lipoproteins (HDL). The within-batch coefficient of variation for immunonephelometry was 6.7% and for electroimmunoassay was 4.7%, but both methods showed similar between-batch precisions (6.9% and 7.0% respectively). Plasma from 122 subjects (62 male, 60 female) were analysed by both methods. Results showed good correlation but immunonephelometry gave consistently lower apoA-I values (mean +/- 1 S.D. = 1.32 +/- 0.25 g/l) than did electroimmunoassay (1.56 +/- 0.33 g/l). Plasma apoA-I concentrations obtained by both procedures were significantly higher (P less than 0.002) in females than in males. Both assays gave results which were highly correlated with high density lipoprotein cholesterol. Immunonephelometry is therefore considered to be satisfactory for estimation of plasma apoA-I concentration.

Apolipoproteins↗
PubMed1979

Response of plasma lipoproteins and acute phase proteins to myocardial infarction.

Plasma concentrations of lipoprotein-lipids, apolipoprotein B (apoB) and of seven other proteins have been estimated serially in 27 patients up to three months following myocardial infarction. Results were compared with those from age- and sex-matched control subjects. At three months the mean total, low density lipoprotein (LDL) and high density lipoprotein (HDL) cholesterol concentrations were higher than those of the control subjects, whereas very low density lipoprotein (VLDL) cholesterol, total and VLDL triglyceride, and total and LDL apolipoprotein B concentrations were not significantly different. Relative to concentrations at three months total and LDL cholesterol and apolipoprotein B concentrations fell markedly, and a slight fall occurred in HDL cholesterol following infarction. VLDL cholesterol and total and VLDL triglyceride were decreased only on day one. Albumin and transferrin concentrations were higher and alpha 1-acid glycoprotein was lower at three months than in the control subjects; alpha 2-macroglobulin, caeruloplasmin, haptoglobin and immunoglobulin IgM were not significantly different. Following infarction albumin and transferrin fell, alpha 2-macroglobulin did not change, and alpha 1-acid glycoprotein, caeruloplasmin, haptoglobin and IgM rose. The changes in both lipids and protein are probably part of the general metabolic response to trauma.

Acute Disease↗
PubMed1978

Plasma protein concentrations in hypertriglyceridaemic subjects. Effect of clofibrate and comparison with normal subjects.

Clofibrate, a widely used hypolipidaemic agent was given for twelve weeks to ten subjects with hypertriglyceridaemia. Its effect on lipoprotein-lipids and caeruloplasmin, IgA, IgM, alpha2-microglobulin and transferrin was assessed by comparing analyses at 4, 8 and 12 weeks on therapy with the means of values at two weeks before and at the start of treatment. The normal variation in plasma proteins was assessed in six healthy volunteers during the same period of time. On clofibrate, very low density lipoprotein (VLDL) cholesterol and triglyceride concentrations fell, but the concentrations of cholesterol in low density (LDL) and high density (HDL) lipoproteins showed no consistent change. Caeruloplasmin and IgM concentrations decreased significantly, IgA showed a limited falls (significant only at 8 weeks) and alpha2-macroglobulin did not change. The concentration of transferrin increased on therapy. No relationships were found between the falls in VLDL-lipid concentrations and the alterations in other plasma proteins. No significant variation occurred in the concentrations of lipids or proteins in the normal subjects during the period of study. The results indicate that clofibrate exerts general effects on protein metabolism.

Adult↗
PubMed1978

Effect of clofibrate on the composition of very low and low density lipoprotein subfractions in type III hyperlipoproteinaemia.

The effect of clofibrate on the lipid and protein composition of very low and low density lipoprotein subfractions (VLDL of SF greater than 100, 60-100 and 20-60; LDL of Sf 10.4-20, 5.7-12 and 3.5-6.5), was investigated in 6 patients with type III hyperlipoproteinaemia (HLP). After four weeks of therapy significant reductions occurred in the concentration of cholesterol in each VLDL fraction, and of triglycerides in Sf greater than 100 and Sf 60-100 VLDL. No changes were found in the concentrations of apolipoprotein B or of the total tetramethylurea (TMU) soluble proteins, but in four patients in whom polyacrylamide disc gel electrophoresis of the TMU soluble proteins was carried out, it was found that arginine-rich peptide (ARP) had largely disappeared on therapy. These findings would be in keeping with increased catabolism of VLDL in response to clofibrate. No significant changes were observed in LDL lipid or protein concentrations.

Apolipoproteins↗
PubMed1977

The composition of low (LDL) and very low (VLDL) density lipoprotein subfractions in type III hyperlipoproteinaemia: comparison with normal subjects.

The lipid and protein composition of very low density lipoprotein (VLDL) and low density lipoprotein (LDL) subfractions (Sf greater than 100, 60--100 and 20--60 VLDL and Sf 10.4--20, 5.7--12 and 3.5--6.5 LDL) in six subjects with type III hyperlipoproteinaemia (HLP) was compared to that of 12 normal subjects. In type III HLP all VLDL subfractions contained increased concentrations of cholesterol and triglycerides and were relatively enriched in cholesterol. VLDL of Sf 20--60 also contained and increased concentration of B-protein. The tetramethylurea (TMU) soluble apolipoproteins of the VLDL subfractions were separated by polyacrylamide disc gel electrophoresis. In the subjects with type III HLP the proportion of arginine rich protein (ARP) was increased in all subfractions. The concentrations of cholesterol and triglycerides were increased in the LDL subfraction of Sf 10.4--20 and cholesterol was decreased in LDL of Sf 5.7--12, but the ratios of cholesterol to triglycerides were not significantly different from those in the LDL subfractions of the normal subjects and the protein composition was also similar. These results provide further evidence that in type III HLP abnormalities are not confined to the stage of conversion of VLDL to LDL, but occur throughout the VLDL spectrum.

Adult↗
PubMed1977

Electrophoretic mobility in agarose of very low density lipoprotein subfractions in type III hyperlipoproteinaemia.

The electrophoretic mobilities in agarose gel of the very low density lipoprotein (VLDL) subfractions of Sf greater than 100, 60-100 and 20-60 from six subjects with type III hyperlipoproteinaemia (HLP) have been compared with those from eight normal volunteers. In type III HLP beta or near-beta (slower VLDL) electrophoretic mobility was not necessarily confined to the VLDL fraction of Sf 20-60 in which it may normally be detected.

Adult↗
PubMed1977

Effect of storage conditions of serum lipoproteins on electrophoretic mobility in agarose gel and on lipid composition.

The effect of conditions of storage of sera and lipoprotein fractions on electrophoretic mobility in argarose gel and on lipid composition has been assessed. It was found that sera or lipoprotein fractions may be stored at 4 degrees C in the presence of ethylene diamine tetra-acetate for up to 5 days before electrophoresis or up to 8 days before estimation of cholesterol and triglycerides. Addition of mercurothiolate of p-chloromercuriphenyl sulphonate offerred no further advantage.

Blood Preservation↗
PubMed1973

Experience with a commercial preparation of 125I-labelled human albumin for study of albumin metabolism.

Evaluation of a commercial preparation of (125)I-labelled albumin for use in the study of albumin metabolism is described. In eight subjects with normal albumin metabolism the proportion of the dose of radioiodide excreted was stable throughout a period of 17 days, indicating that there was no excessive denaturation of the iodinated albumin. Characteristics of albumin metabolism-pool sizes, catabolic rate, etc-were in agreement with currently accepted normal values. It is concluded that this preparation of iodinated albumin is suitable for metabolic use.

Adult↗