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Biomedical subjects

F C Law

Publications and source records attributed to F C Law.

At least 19 recordsLinked to original sources

Reliability and reproducibility of a Chinese-language visual function assessment.

OBJECTIVE: The purpose of the study was to validate a Chinese-language visual function assessment within the context of a routine cataract surgery practice and to assess the contribution of the method of questionnaire administration. DESIGN: The visual function assessment (VFA) was translated into Chinese. Two groups of study subjects were recruited: Chinese who did not speak English and Chinese conversant in English. Consecutive preoperative cataract patients of Chinese ancestry presenting to an urban ophthalmology practice were recruited. The questionnaire was administered in person or by telephone interview. Pre-operative visual acuity was recorded. Visual function scores were analyzed to assess reliability and correlation with visual acuity. RESULTS: Among the 186 potential study subjects, 155 patients completed the study The Chinese-language visual function assessment had good internal consistency (Cronbach alpha = 0.97, inter-item correlations = 0.43 to 0.96) . Reliability (with regard to the English version) and test-retest reproducibility of the Chinese questionnaire were strong with intraclass correlation coefficients greater than 0.60. The method of administration contributed to the measures of reliability and reproducibility. CONCLUSION: These results show that a Chinese-language version of the VFA questionnaire is reliable and valid. In industrialized countries with large Chinese-speaking populations and newly developed countries of East and Southeast Asia, the visual function assessment may be helpful in assisting routine clinical patient evaluation and cross-cultural outcome assessment programmes. Our findings also suggest that self-administered visual function assessments may be more reliable and valid than interview-generated assessments.

Aged↗

Use of mitomycin C with conjunctival autograft in pterygium surgery in Asian-Canadians.

OBJECTIVE: To determine whether using mitomycin C (MMC) before applying conjunctival autograft is better than conjunctival autograft alone in preventing the recurrence of pterygia in an Asian-Canadian population. DESIGN: Nonrandomized, retrospective, comparative case series. PARTICIPANTS: A total of 159 patients were included in the study. Seventy patients (76 eyes) received MMC (0.25 mg/ml for 1 minute) with conjunctival autograft; 89 patients (98 eyes) received conjunctival autograft alone. INTERVENTION: All patients had primary (first-occurrence) pterygia excised and conjunctival autograft applied with or without MMC adjunct. MAIN OUTCOME MEASURES: Recurrence of pterygia was monitored for up to 1 year after the operation. Any complications (e.g., scleral thinning and necrosis) were documented. RESULTS: Patients' pterygia were examined on presentation and were graded 1 through 3. Grade 1 pterygia were fibrovascular proliferations extending up to one quarter the diameter of the cornea; grade 2 extended between one quarter to one half the distance across the cornea; and grade 3 extended beyond the visual axis. In the more severe pterygia group (grades 2 and 3 combined), there was significantly less pterygium recurrence in the MMC/autograft group (7%) compared with the autograft alone-treated group (26%). There was no significant difference in recurrence between groups for less severe grade pterygia (grade 1). The recurrence rate of all pterygia in the MMC/autograft group was 9% compared with 18% for the conjunctival autograft group. This difference was not statistically significant, however, because of a small sample size. There were no significant complications in either group and no difference between groups in complication rates. CONCLUSIONS: The authors suggest using MMC in patients with more severe pterygia as an adjunct to conjunctival autograft to lower the recurrence rate. Combining the use of MMC with conjunctival autograft allows for decreased dosage and time of intraoperative exposure of mitomycin, thereby making it safer for application.

Asia↗

Evidence of 14C-furazolidone metabolite binding to the hepatic DNA of trout.

Furazolidone (FZ) is a nitrofuran drug commonly used in aquaculture. In the present study, [methylidene-14C]-FZ or [oxazolone-4,5-14C]-FZ was offered to rainbow trout (Oncorhyncus mykiss) in medicated feed at a daily dose of 135 mg/kg b. wt. for 10 days. The trout were sacrificed at specific time points post-dosing and the liver removed for DNA-bound 14C characterization. Both forms of the 14C-labelled FZ were converted by trout to reactive metabolite(s) which bound irreversibly to the hepatic DNA. The amount of 14C bound to the hepatic DNA increased with post-dosing time and was higher in trout pretreated with [methylidene-14C]-FZ than in trout pretreated with [oxazolone-4,5-14C]-FZ. The identity of the FZ reactive metabolite(s) remained to be elucidated. However, a part of the FZ reactive metabolite(s) could be released as 3-amino-2-oxazolidone by acid hydrolysis. An appreciable amount of 14C was also found to bind irreversibly with the hepatic DNA of trout following an i.v. injection of [oxazolone-4,5-14C]-FZ. Results of these studies indicate that FZ is metabolized by trout to a reactive metabolite(s) which binds irreversibly to the DNA of trout liver.

Animal Feed↗

Nitrite mediated T lymphocyte responses in the intestinal immune system of mice infected with Trichinella spiralis nematode.

The effects of orally administered sodium nitrite (20 mg NaNO2/kg b. w) on the responses of T and B lymphocytes collected from the mesenteric lymph nodes were studied in resistant AKR/J, H-2(k) haplotype mice infected with Trichinella spiralis nematode. On days 6, 9, and 12 postinfection, the mesenteric lymph node cells (MLNC) were collected from the mice and assayed for lymphocyte subsets (CD4(+), CD8(+), B220(+)), cytokines (IL-2, IL-5), and INF-gamma. At the same time, the number of adult worms in the small intestine were counted. Infection of the nitrite-treated mice with T. spiralis L1 larvae caused a marked increase in the number of adult worms in the small intestine. However, preincubation of T. spiralis L1 larvae with nitrite before infecting the mice resulted in a significant reduction in the number of adult worms (p < 0.05). Preincubation of T. spiralis L1 larvae with nitrite also caused an increase in the number of CD4(+) and CD8(+) cells as well as IL-2, IL-5, and INF-gamma levels. An increased level of CD8(+) subsets and a depression of IL-2 and IL-5 production by MLNC were observed in mice infected with larvae without nitrite pretreatment. Since supplementary rIL-1alpha was found to alter INF-gamma secretion by MLNC in vitro, the pattern of MLNC proliferation was examined further with the nitrite-treated mice. Sodium nitrite increased thymidine incorporation into the MLNC. However, INF-gamma production was not enhanced when rIL-1alpha was added to the MLNC culture obtained from nitrite-treated mice.

Administration, Oral↗

Biotransformation and elimination of 1-nitropyrene in the isolated perfused lung: effects of pretreating rats with phenobarbitone, beta-naphthoflavone, benz(A)anthracene or their mixtures.

The isolated perfused rat lung (IPL) was perfused with 60 ml of recirculating Krebs-Ringer solution containing 150 micrograms of 1-nitropyrene (1-NP) for 1 h. The 1-NP was administered to the IPL by the intratracheal or intravascular route. At specific time points after 1-NP administration, perfusate samples were removed from the IPL and analysed for 1-NP and its metabolites by HPLC. Monohydroxynitropyrenes, dihydroxynitropyrenes and 1-NP were found to be present in the perfusate. The time course of 1-NP concentrations in the perfusate could be described by a one-compartment pharmacokinetic model. Pretreatment of rats with beta-naphthoflavone (BNF), benz(a) anthracene (BA) or a mixture of phenobarbitone (PB) and BNF (PB + BNF) significantly enhanced the metabolism of 1-NP and decreased the mean residence time (MRT) of 1-NP in the perfusate. Pretreatment of rats with these mixed-function oxidase inducers also increased significantly the absorption of 1-NP by the lung when it was administered intratracheally. In contrast, pretreatment of rats with PB did not appear to have any effect on the pharmacokinetics of 1-NP in the IPL.

Animals↗

A urine collection system for studying the excretion of xenobiotics from Dungeness crabs.

A technique is described for collecting urine from the Dungeness crab, Cancer magister (Dana), a commercially important seafood and an indicator species of aquatic pollution. The technique has been modified from previously published methods to study the role of urinary excretion in the elimination of lipophilic pollutants by the Dungeness crab. The improved urine collection system uses chemically resistant and nonreactive materials that are better suited to pharmacological and toxicological studies than those used in previous methods. The improved method was tested by injecting a vital dye into catheterized Dungeness crabs and by measuring urine flows for 10 days. This technique can be used to determine the urinary excretion of xenobiotics and facilitate the characterization of chemicals and their metabolites accumulated by crabs.

Animals↗

Pharmacokinetics of inhaled pyrene in rats.

Male Wistar rats were exposed to micronized aerosol concentrations of a 14C-labeled model polycyclic aromatic hydrocarbon (pyrene) at 200, 500, and 800 mg/m3 for a period of 95 min. Both the 14C label and free pyrene were monitored in the blood, urine, and feces. At the termination of the blood sampling, three of the six rats per dose group were killed and the distribution of [14C]pyrene to eight major tissues was analyzed. The analysis of blood concentration data using a one-compartment pharmacokinetic model revealed that the uptake and elimination kinetic parameters were dose dependent, for both total radioactivity (pyrene plus metabolites) and for pyrene per se, over the range of exposures used in this study. The ratio of the percent excreted via the urinary and fecal routes, collected over a 5-d period postexposure was about 1.0 at each exposure level.

Absorption↗

Urinary and biliary metabolites of pyrene in rainbow trout (Oncorhynchus mykiss).

1. Pyrene was administered i.p. as a single dose to trout (Oncorhynchus mykiss). Urine was collected continuously for 3 days and bile sampled at the end of this period. Pyrene metabolites in these biological fluids were identified by 1H-nmr spectrometry, glc-ms and hplc-ms. 2. 1-Hydroxypyrene was the major oxidation metabolite in the urine and bile. Small amounts of 1,6-dihydroxypyrene and a putative 1,8-dihydroxypyrene metabolite also were detected. Unchanged pyrene was not found in any of these biological fluids. 3. Both free and conjugated metabolites of pyrene were found in the bile and urine. The majority of the pyrene metabolites in the bile were conjugated with glucuronic acid or sulphate.

Animals↗

Percutaneous uptake, distribution, and excretion of pyrene in rats.

Groups of 12 male Wistar rats, of about 400 g body weight, were dosed with 2, 6, or 15 mg/kg of 14C-labeled pyrene, dissolved in acetone, applied to 4 cm2 of a shaved area of the mid back. Three animals in each dose group were killed at 1, 2, 4, and 6 d post-dosing, and their principal organs were removed and analyzed for pyrene and [14C]pyrene equivalents. Urine and feces, as well as the area of skin to which the dose was applied, were also analyzed for [14C]pyrene equivalents. The rate of uptake from the skin was rapid (t1/2 0.5-0.8 d) relative to rate processes for the other organs, and about 50% of the applied dose was excreted over the 6 d of the study. The significant decrease in the fraction of the dose excreted and in the normalized amounts distributed to the various organs and tissues, as the dose increased for all chemical species measured, was strongly suggestive of nonlinear kinetics, as has been observed in previous studies. Levels of pyrene were highest in the liver, kidneys, and fat. Levels of metabolites were also high in the lung. It was evident that the dermal route of uptake was not insignificant for this model polycyclic aromatic hydrocarbon and may represent a significant exposure route for exposed humans.

Adipose Tissue↗

Biocompatibility of particulate polymethylmethacrylate bone cements: a comparative study in vitro and in vivo.

The biocompatibility of particles of four different commercial polymethylmethacrylate (PMMA) bone cements was evaluated by exposing human synovial fibroblasts and mouse peritoneal macrophages to particles of cement. Cell integrity and inflammatory potential were assessed using enzyme release and microscopical examination. Results suggested the occurrence of cell damage in both cell types and macrophage studies indicated inflammatory potential. The response in vivo was investigated by intra-articular injection of the particles into mouse knee joints. Clinical and histological evaluation was performed over 2-52 wk. Particles of all four cements were well tolerated in the joints.

Acetylglucosaminidase↗

A biologically based toxicokinetic model for pyrene in rainbow trout.

A biologically based toxicokinetic model was developed to stimulate the metabolic disposition of pyrene in trout with an average body weight of 450 g and dosed with a single bolus injection of the chemical (10 mg/kg). The model consists of a membrane-limited muscle compartment and six flow-limited compartments including the gills, liver, gut, kidney, carcass, and blood. The compartments are represented by mass balance equations including terms for the binding of pyrene to tissue and blood proteins, biotransformation, penetration rate into the muscle, blood flow rate, tissue mass, etc. The model also provides for nonsaturable and saturable clearances of pyrene by the liver and kidney. Michaelis-Menten constants for pyrene metabolism (Km, Vmax) were determined from in vitro experiments using isolated liver cells. Renal clearance of pyrene was very close to the glomerulus filtration rate of trout. Solution of the system of equations yielded the time courses of pyrene concentration in the tissues. Predicted concentrations of pyrene in the gills, liver, gut, kidney, muscle, and blood were consistent with experimental observations for at least 6 days. The model was validated by comparing the model predicted and experimental results of trout weighing 285 g and dosed with a single intraarterial dose (3 mg/kg) of pyrene. The predicted pyrene concentrations also were in adequate agreement with the empirical data.

Animals↗

Biocompatibility of particles of GORE-TEX cruciate ligament prosthesis: an investigation both in vitro and in vivo.

This research was to determine the biocompatibility of simulated wear particles from a GORE-TEX cruciate ligament prosthesis using in vitro and in vivo techniques. Exposure of normal human synovial fibroblasts and primary mouse peritoneal macrophages to the particles revealed no cytotoxicity, as determined by lactate dehydrogenase release, but beta-N-acetyl-D-glucosaminidase was released from macrophages at high concentrations, showing inflammatory potential. This was not seen when the particles were injected into mouse knees, where no adverse reactions were observed. These simulated wear particles were shown to be biocompatible using these in vitro and in vivo systems.

Acetylglucosaminidase↗

Biocompatibility of diamond-like carbon coating.

Diamond-like carbon coating is an inert, impervious hydrocarbon coating with properties suitable for use in the biomedical field, particularly in orthopaedic implants. Mouse peritoneal macrophages and mouse fibroblasts were grown on tissue culture plates treated with diamond-like carbon and the biocompatibility assessed both biochemically and morphologically. This investigation showed that diamond-like carbon coating caused no adverse effects on cells in culture and therefore merits further investigation as a coating for biomedical use.

Animals↗

Pharmacokinetics and bioavailability of pyrene in the rat.

Groups of 6 male Wistar rats, of about 400 g body weight, were dosed with 14C-labeled pyrene, dissolved in an Emulphor/water solvent vehicle, at 5 different dose levels by the intravenous or oral routes. Appropriate mathematical models were fitted to blood concentration-time data for [14C]pyrene and pyrene per se and dose-trend analyses were carried out. Areas under these curves were used to assess the bioavailability of the orally administered doses. Tissue concentrations, measured at the termination of the blood sampling period, gave a quantitative measure of the distribution of the administered dose. Attempts to repeat these studies with similar doses of tritium-labeled benzo[a]pyrene were frustrated by the lack of meaningful blood-level data. Dose trends for the derived pharmacokinetic parameters for pyrene revealed that the kinetics were nonlinear and strongly suggestive of enterohepatic recycling. Biliary excretion, measured in a separate experiment, gave support to this hypothesis. The bioavailability of the orally administered doses was between 50 and 60%. Over a 6-d period postdosing, some 45 and 40% of the administered dose was excreted via the urine and feces, respectively, irrespective of the route of administration. Distribution to the tissues of the 14C-label was highest in the perirenal fat, intermediate in the liver, kidneys, and lungs, and lowest in the heart, testes, spleen, and brain.

Administration, Oral↗