Biomedical subjects

F Caboi

Publications and source records attributed to F Caboi.

3 recordsLinked to original sources

PubMed2004

Biocompatible lipidic formulations: phase behavior and microstructure.

Biocompatible systems formulated for use in the food, cosmetic, and pharmaceutical fields are characterized. Ternary phase diagrams of mixtures of natural lipids (glycerol trioleate, glycerol monooleate, diglycerol monooleate, and lecithin) and water were investigated by means of optical microscopy in polarized light and by multinuclear NMR spectroscopy. All systems showed a microemulsion region at high oil content and a large area of coexistence of two liquid crystalline (hexagonal and lamellar) phases. 1H and 13C NMR self-diffusion measurements were used to characterize microstructural features of the microemulsions. On water dilution, the two-phase liquid crystalline region transforms into a creamy emulsion area where the droplets of water are stabilized by both the lamellar and the hexagonal phases, as indicated by 2H NMR measurements. Due to the very effective dispersing action of the two liquid crystalline phases, these emulsions show a high stability toward phase separation.

Biocompatible Materials↗

PubMed2001

Addition of hydrophilic and lipophilic compounds of biological relevance to the monoolein/water system. I. Phase behavior.

The solubilization of hydrophilic and lipophilic molecules, with biological relevance, in the monoolein/water (MO/W) system has been investigated for phase behavior. Small angle X-ray scattering (SAXS), nuclear magnetic resonance (NMR) and optical microscopy (OM) have been used to characterize the microstructure of the liquid crystalline phases. Partial phase diagrams of the MO/W system in the presence of sodium decanoate, 1-adamantanamine hydrochloride, decanoic and dodecanoic acids, acetyl salicilic acid and retinol have been determined. The stability of the various phases has been followed for at least eight months. The polarity and the molecular structure of the additive determine whether it is located at the polar interface or in the apolar region of the lipid layer. Therefore, the additive affects the interfacial curvature of the lipid layer differently, which in turn will trigger transition to disparate phases. A cubic-to-reverse hexagonal phase transition has been observed with time for most of the ternary systems, with the exception of 1-adamantanamine hydrochloride and retinol. The release of free glycerol and oleic acid due to MO hydrolysis has been clearly demonstrated by 13C NMR. This would account for the changes in phase behavior observed with time. The released oleic acid, located in the MO acyl chain region, favors the inverse interfacial curvature. The average lipid dimensions in the cubic and in the reverse hexagonal phases have been calculated from SAXS data.

Carbon Isotopes↗

PubMed2001

Addition of hydrophilic and lipophilic compounds of biological relevance to the monoolein/water system II - 13C NMR relaxation study.

The addition of hydrophilic and hydrophobic molecules to the 1-monooleoyl glycerol (MO)/water (W) system has been investigated at a molecular level by 13C nuclear magnetic resonance (NMR) relaxation. Depending on the nature of the additive, the liquid crystalline phases of the MO/W binary system are modified. The 13C NMR spin lattice relaxation rates of the various MO carbons were determined in the presence of the additives for different types of L(2) and liquid crystalline phases. Data revealed that local dynamics are independent of type and amount of additive (within 5 wt.%), and also of the type of the structural arrangement. The curvature of the interface does not affect the local mobility of MO carbons, with the exception of the glycerol G3 and the carboxylic C1 carbons. Moreover, the presence of the double bond in the mid part of the hydrocarbon chain induces a levelling in the relaxation rates on the neighboring carbons. The 13C NMR spin lattice relaxation rates at two magnetic field strengths and the Overhauser enhancement were measured in the L(2) phase of the MO/W/sodium decanoate system. The use of a two-step model of relaxation allowed to estimate order parameters, and slow and fast motions of MO in the structured aggregate.

Gels↗