Biomedical subjects

F Capasso

Publications and source records attributed to F Capasso.

6 recordsLinked to original sources

PubMed1976

Effects of modification of intracellularcyclic AMP levels on the imediate hypersensitivity reaction in vivo.

Experiments were performed to study the effects of agents that elevate endogenous cyclic AMP levels on the progression of a reverse passive Arthus reaction in the pleural cavity of rats. Three hours after the onset of the reaction a marked reduction in histamine release was observed which correlated well with increases in cellular cyclic AMP concentration. The results at 6 hr suggested a relationship between cyclic AMP and leucocyte PGE, the latter being reduced dramatically both in the cells and in the fluid exudate by agents that elevate cyclic AMP. It was concluded that cyclic AMP may play an important regulatory role in vivo in the release of inflammatory mediators from leucocytes.

Animals↗

PubMed1975

Further studies on carrageenan-induced pleurisy in rats.

A study has been made of the exudate provoked by intrapleural carrageenan in rats. Various parameters have been measured including volume of exudate, numbers and types of cells. Pharmacological assay has shown the early release of histamine and 5HT into the exudate followed at later times by a release of prostaglandins, and a second release of histamine. The role of complement has been studied. Intrapleural injection of carrageenan after complement depletion produced a reduced migration of polymorphonuclear cells and also a reduction in volume of exudate. Prostaglandins remained unaffected by this treatment.

Animals↗

PubMed1975

Quantitative studies on cell-mediated immunity in the pleural cavity of guinea-pigs.

Intrapleural injection of PPD into CFA-sensitised guinea-pigs produced a reaction having many of the characteristics of classical delayed hypersensitivity. Quantitative investigations of the time course of this reaction showed that it was typified by an initial delay in the onset of increased vascular permeability, which reached its peak between 12 and 18 hr. The cellular response in the pleural cavity followed a similar pattern, attaining maximum levels between 18 and 24 hr. Mononuclear cells dominated the reaction from 12 to 48 hr. The histological appearance of the lung tissue was also studied. Treatment with Cobra venom factor, a complement-depleting agent, failed to modify this reaction. Cyclic AMP levels were assayed for both the cellular and cell-free exudates.

Animals↗

PubMed1975

Pharmacological mediators of various immunological and non-immunological inflammatory reactions produced in the pleural cavity.

Intrapleural exudates have been produced in rats and guinea-pigs using calcium pyrophosphate, cell mediated immunity, reversed passive Arthus reaction and carrageenan. The intracellular concentration of various pharmacological mediators has been measured at different times during the evolution of these inflammatory responses. These have been correlated with volume of exudate and cellular migration. The significance of these findings has been discussed and the possible application to the search for new anti-inflammatory agents and their use in diagnostic procedures.

Animals↗

PubMed1975

Calcium pyrophosphate-induced pleurisy in rats: a new model of acute inflammation.

A model is described of acute inflammation in the pleural cavity of rats using calcium pyrophosphate as the irritant. This model would seem to simulate the pseudogout syndrome. It has been shown to be acute in onset, dominated by polymorphonuclear cells, complement independent. The advantage of the model is that volume of exudate, numbers and types of cells may be quantitated. Prostaglandins and cyclic AMP have been measured in the migrating cells. The significance of these findings has been discussed.

Animals↗

PubMed1975

A model for the quantitative study of Arthus (immunologic) hypersensitivity in rats.

A model of reverse passive Arthus (RPA) reaction in the pleural cavity of rats is described. The time course of development of exudate and migration of cells has been examined. It has been found to be complement dependent and dominated by polymorphonuclear cells. The reaction reaches a peak around 6 hours after challenge. Cyclic AMP levels have been measured both intracellularly and extracellularly and have been found to persist at high levels after the waning of the reaction.

Animals↗