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F Caracciolo

Publications and source records attributed to F Caracciolo.

78 records · Page 5Linked to original sources
PubMedDate not supplied

Glutathione-S-transferase activity and multidrug resistance phenotype in chronic lymphocytic leukemia: do they have any clinical relevance?

BACKGROUND: Lymphocytes from patients affected by B-cell chronic lymphocytic leukemia (B-CLL) have frequently been shown to be positive for the multidrug resistance (MDR) phenotype. However, this phenotype does not seem to be responsible for the resistance to alkylating agents usually employed in the management of CLL. METHODS: Lymphocytes from 42 patients were evaluated by flow cytometry for P-170 expression and by spectrophotometry for glutathione-S-transferase (GST) activity. RESULTS: Our findings show that GST is not related to any clinical parameter but is increased in treated patients. Conversely, 85% of patients were positive for P-170 and this was related to the percentage of CD5/CD19-positive lymphocytes. CD5/CD19-negative patients were also negative for P-170. MDR was not related to any clinical parameter evaluated nor to GST activity in lymphocytes. CONCLUSIONS: MDR is constitutively expressed in B-cell chronic lymphocytic leukemia and seems to be related to a CD5/CD19 B-CLL phenotype. The increase of GST activity in treated patients is statistically significant (p < 0.005).

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMedDate not supplied

Granulocyte colony-stimulating factor (G-CSF) administration increases PMN CD32 (FcRII) expression and FcR-related functions.

The phenotypical and functional properties of circulating neutrophils from ten patients suffering from intermediate- and high-grade non-Hodgkin lymphoma were investigated before and after rhG-CSF administration (5 micrograms/kg/day subcutaneously for 5 days). The following parameters were studied: flow cytometry evaluation of surface CD32, CD16, CD11b and CD18 by means of a whole blood method; whole blood phagocytosis by means of a flow cytometric assay; whole blood chemiluminescence using opsonized zymosan as a stimulus. A significant increase in the expression of surface CD32 was detected in all patients, while CD11b expression was found to be increased in only four of them. CD16 and CD18 expression did not change. A significant enhancement of phagocytosis and phagocytosis-associated chemiluminescence was also observed. These results show that rhG-CSF administration can increase both FcRII expression and FcR-related functions.

Adult↗
PubMedDate not supplied

[Surgical protection: anatomic and diagnostic contribution of a new CT techniques in preoperative study of the temporal bone].

In the last ten years, CT study of the temporal bone has become, finer and has enriched itself with new projections. The objective of this paper fruit of collaboration between radiologist and otologist, is the search for CT projections, which evidentiate, with the same order and progression, the most important reference points that otologists find in temporal bone surgery. The study was conducted on fresh temporal bone extracted from cadavers which underwent tomography according to a projection very similar to a surgical plane defined precisely as "surgical". The Authors comment on what they consider the three most significant tomographies: those taken from the cortical mastoid at 15, 23 and 32 mm. The various anatomical structures and the relationships between them are identified with an operatory logic; this would afford in the surgical planning phase a better evaluation of the most appropriate approach, of the risks this approach would involve and of the length of the surgical procedure. The Authors also studied and developed an accessory that allows the application of surgical projection in living patients.

Humans↗