Biomedical subjects

F Castagna

Publications and source records attributed to F Castagna.

21 records · Page 2Linked to original sources

PubMedDate not supplied

Comparison of GLC-EMIT analysis for the assay of methadone and its major metabolite in urine.

The enzyme-multiplied immunoassay technique (EMIT) was compared with the gas-liquid chromatographic (GLC) method in the analysis of 130 urine samples from subjects receiving methadone treatment and non-methadone controls. The GC method allowed the quantitation of 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, and major metabolite of methadone. A good correlation was found between the two methods. The advantages of using the EMIT system are discussed.

Chromatography, Gas↗

PubMedDate not supplied

Solid-phase extraction and HPLC-UV confirmation of drugs of abuse in urine.

A series of six liquid chromatographic methods were developed to confirm the presence of six classes of drugs of abuse in urine. The chromatographic separations were performed with a reversed-phase C8 column, except in the case of morphine, which was separated on a normal phase column. Isocratic and gradient elutions, ion pair technique, and UV detection were employed. Sample pretreatment involved the extensive application of solid-phase extractions and liquid-liquid extractions on solid supports. The specificity and sensitivity enabled the confirmation of morphine, benzoylecgonine, THC-COOH, amphetamine, and methamphetamine, six barbiturates, and nine benzodiazepines screened positive by EMIT in urine.

Amphetamine↗

PubMedDate not supplied

Direct and indirect homogeneous enzyme immunoassay of benzodiazepines in biological fluids and tissues.

The evaluation of new homogeneous enzyme immunoassay (EMIT) for benzodiazepines is described. The assay, although developed for direct analysis in serum, has been used to detect benzodiazepines in other body fluids and tissues; this is accomplished through two processes. The first consists of a previously reported technique of extraction and reconstitution of the original specimen. The second consists of dilution of the original specimen. The assay is compared to a gas chromatographic procedure.

Benzodiazepines↗