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Biomedical subjects

F Cattabeni

Publications and source records attributed to F Cattabeni.

At least 217 records · Page 12Linked to original sources
PubMed1972

Gas chromatographic-mass spectrometric assay of four indole alkylamines of rat pineal.

Gas chromatography-mass spectrometry was used to quantitate serotonin, N-acetylserotonin, 5-methoxytryptamine, and melatonin in single rat pineal glands. After gas chromatographic separation, the ion density of specific fragments of each indole was measured with mass spectrometry. Sensitivity of this indole assay is of the order of 10(-12) to 10(-13) mole. Routinely, specificity is based on gas chromatographic retention time and the recording of the ion density generated by specific fragments. Absolute identification of the extracted indoles was based on multiple ion detection.

Animals↗
PubMed1972

Norepinephrine and dopamine: assay by mass fragmentography in the picomole range.

Gas chromatography-mass spectrometry makes possible the simultaneous measurement of norepinephrine and dopamine in concentrations of 0.1-milligram tissue samples. Specificity of the assay is confirmed both by the retention time of the compound and by the mass to charge ratio of the fragments recorded. The sensitivity is of the order of 0.5 picomole, and linearity of the response is maintained up to at least 200 picomoles.

Animals↗
PubMedDate not supplied

The effect of K2Cr2O7 on the growth and morphology of Escherichia coli.

Treatment with 16 microM K2Cr2O7 results is a time-dependent loss of the clonogenicity of E. coli cells. Under the same experimental conditions, optical density values do not change significantly, since cells have the ability to replicate but not to septate. In fact, microscopic analysis of samples taken over 240 min of exposure to the toxin has revealed the formation of filaments. The filamentous response appears similar to that generated by mitomycin C.

DNA Damage↗
PubMedDate not supplied

Role of metal ions in oxidant cell injury.

This paper represents a short review of recent data on the molecular mechanism(s) of H2O2 cytotoxicity. The role of metal ions has been discussed in light of their ability to drive a reaction of the Fenton type. Hydroxyl radicals play a key role in mediating the deleterious effects of the oxidant, although these species do not seem to directly produce the lethal lesions. It still remains unclear whether or not DNA represents a critical target for H2O2-induced cytotoxicity.

Animals↗
PubMedDate not supplied

Levels of NGF, p75NGFR and ChAT immunoreactivity in brain of adult and aged microencephalic rats.

Methylazoxymethanol (MAM)-induced microencephalic aged animals with reduced cortical mass and unmodified basal nucleus were used to study the relationship between cells that produce and cells that utilize NGF. Total cortical ChAT activity of MAM 2, 19 and 27 month old animals was reduced compared to their age-matched controls. To verify whether the reduction of enzyme activity can be ascribed to changes in or ablation of projecting neurons, we carried out immunohistochemical analysis of ChAT and low affinity NGF receptor (p75NGFR) in the basal nucleus of control and MAM-treated animals. ChAT and p75NGFR immunostaining of basal forebrain cholinergic neurons showed morphological changes in MAM animals, as revealed by cellular atrophy, reduced dendritic arborization and decreased staining intensity. In the cerebral cortex of microencephalic animals, reduced levels of NGF compared to controls were observed at all examined ages. These results suggest that MAM treatment induces long-lasting ablation of cortical NGF-synthesizing cells leading to reduced trophic support to basal forebrain cholinergic neurons, which might be responsible for the cellular atrophy observed in the basal nucleus.

Aging↗
PubMedDate not supplied

Nocturnal hyperactivity induced by prenatal methylazoxymethanol administration as measured in a computerized residential maze.

Treatment of female Sprague-Dawley rats with 15 or 25 mg/kg (IP) of methylazoxymethanol acetate (MAM) at gestational day 15 (15 DG) resulted in a dose-dependent reduction of total brain weight of the adult offspring. When tested for spontaneous activity in a residential maze over a 23 hour period, those animals treated with the highest dose of MAM showed an increase in both locomotion and local activity during night hours without changes in the structure of behavior. Animals treated with 15 mg/kg of MAM showed no difference in activity compared to controls despite a significant reduction in brain weight.

Animals↗