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Biomedical subjects

F Ciampor

Publications and source records attributed to F Ciampor.

At least 73 records · Page 4Linked to original sources
PubMed1977

The effect of supraoptimal temperature on the formation of pseudorabies virus particles.

Replication of virulent and attenuated strains of pseudorabies virus (PRV) at supraoptimal temperature was inhibited in L cells non-adapted to 40 degrees C. Electron microscopy revealed the prevalence of hollow core particles without nucleoid; both the nuclei and cytoplasm of L cells were markedly altered at supraoptimal temperature. In BHK-21 cells adapted to 40 degrees C only replication of attenuated strains was inhibited whereas the virulent ones reached high titres at this temperature. The particles produced at 40 degrees C by attenuated strains were non-infectious but morphologically normal. The ability of attenuated PRV strains to reproduce in adapted BHK-21 cells at 40 degrees C was in correlation with the degree of their attenuation. Changes in the nuclei and cytoplasm of adapted BHK-21 cells at supraoptimal temperature were not as marked as in L cells.

Animals↗
PubMed1976

Morphological, cytological and biological observations on viruses isolated from patients with subacute thyroiditis de Quervain.

New data on viruses isolated from patients with subacute thyroiditis de Quervain are reported. Characteristic morphological, cytological, some physico-chemical and biological features of the isolated viruses are described. A possible role of these viruses in human and animal health disorders is discussed. The isolated viruses remain unclassified so far.

Animals↗
PubMed1976

Infection of Hela cells with a virulent and an attenuated strain of pseudorabies virus studied by electron microscopy.

Hollow-core particles, forming crystals in nuclei, prevailed in HeLa cells infected with an attenuated strain of pseudorabies virus (PRV). After infection with a virulent PRV strain, the cells contained mainly fully infectious dense-core particles. These findings might explain the lower susceptibility of HeLa and some other human cells to infection with attenuated strains of PRV as compared to virulent strains.

Cell Nucleus↗
PubMed1975

Experimental latent herpesvirus infection in rabbits, mice and hamsters: ultrastructure of the virus activation in explanted gasseric ganglia.

The frequency of latent infection as established in trigeminal ganglia of rabbits, mice and hamsters with human herpesvirus type 1 (HVH) was compared using two different virus strains. Explantation proved to be effective in reisolation of HVH from ganglion tissue, which did not yield infectious virus at time of its removal. After healing of acute keratitis, the latent infection in homolateral gasseric ganglia of rabbits was detected at a relatively high frequency (60-80 per cent) up to 120 days post infection (p.i.) in case of both virus strains. The activation rate was a little lower in hamsters. After inoculation of suckling and young mice with a sublethal dose of HVH by oral and nasal routes, approximately 40-100 per cent of the animals had virus in their gasseric ganglia during the acute period; 30-60 days later only 10-25 per cent had virus in the latent form. Immunofluorescent and electron microscopic examination of the explanted ganglion tissue showed the presence of HVH in neurons, neuronal satellites and Schwann cells. The nuclei of noneural cells contained numerous crystalline arrays. The possibility that pseudounipolar neurons of the regional sensoric ganglion are not the exclusive site of HVH latency is discussed.

Animals↗
PubMed1975

Morphology of A/WSN influenza virus-infected chick embryo cells.

A/WSN (H0N1) influenza virus-infected chick embryo cells (CEC) attained a swollen central region 4 hours post infection (p.i.), different from the elongated uninfected cell. From 6 hours p.i. of CEC an increasing number of approximately 100-120 nm, round protrusions were observed by scanning and transmission electron microscopy on the cell membrane, which was smooth on the uninfected cell. At 48 hours p.i. a great number of protrusions were observed on the cell; in addition, there were a number of holes or invaginations in the membranes of the infected cells indicating cell destruction.

Animals↗