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F Ciani

Publications and source records attributed to F Ciani.

77 records · Page 5Linked to original sources
PubMedDate not supplied

Cell proliferation and growth-associated protein 43 expression in the olfactory epithelium in Poecilia reticulata after copper solution exposure.

This study investigated the regeneration in the olfactory mucosa of the teleostean fish Poecilia reticulata when returned to dechlorinated tap water after 4-day exposure to 30 microg/L of Cu(2+). The regeneration process in the olfactory tissue was examined in fishes at 0, 3, 6 and 10 days of recovery in well water. Jade B staining permitted to evaluate the rate of the damage which was especially extended to olfactory neurons. Immediately after the end of exposure, a massive mitotic activity in the basal region of the mucosa was detected by immunostaining with PCNA. After 3 days of recovery the nuclei of the newly formed cells had already finished their migration to the upper portion of the epithelium, and cellular division was much less intense. Simultaneously, immunoreactivity for the neural growth-associated phosphoprotein GAP-43 increased respect to control levels, revealing that the new differentiating PCNA-positive elements belonged to immature neurons. After 6 days in well water no mitotic activity was detected, while the GAP-43 labelling appeared particularly concentrated in the apical surface of the olfactory epithelium. After 10 days the aspect of the olfactory epithelium was almost identical to the control. The present results suggest that after 10 days regeneration seems to be complete and integrity of the tissue restored. Furthermore, the epithelium reconstitution does not show apparent divergence from other fishes or mammals.

Animals↗
PubMedDate not supplied

[Hyperglycinemia in clinical-laboratory practice].

Hyperglycinemia is a non rarely observed biochemical finding which can be caused by a primary defect of the glycine cleavage system (nonketotic hyperglycinemia) or by an enzymatic block due to toxic metabolites (ketotic hyperglycinemia in organic acidurias) or to specific drugs (such as sodium valproate). The Authors report their clinical and laboratory experience in the diagnosis of different forms of hyperglycinemia. Three patients with nonketotic hyperglycinemia (1 patient with transient neonatal form, 1 with the classic neonatal form, and 1 with late onset form), 6 patients with ketotic hyperglycinemia (1 propionic acidemia, 5 methylmalonic acidurias), and 8 patients with hyperglycinemia during sodium valproate treatment are described. In this study the diagnostic iter and the importance of a precise diagnosis of the hyperglycinemia for the appropriate treatment are discussed.

Amino Acid Metabolism, Inborn Errors↗