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F Cluzeaud

Publications and source records attributed to F Cluzeaud.

43 records · Page 3Linked to original sources
PubMed1984

Heterogeneity of uridine incorporation along the rabbit nephron. I. Autoradiographic study.

We present here an autoradiographic study of uridine labeling in tubular segments micro-dissected from the rabbit kidney. Kidney pyramids were incubated for 60 min with low (66 nM) and high (66 microM) [3H]-uridine concentration. At the two concentrations studied the labeling was almost exclusively nuclear in all segments studied. At the low concentration, labeling predominated in the macula densa (MD = 63.88 +/- 6.15 silver grains/100 micron2, n = 11), cortical ascending limb (CAL = 19.65 +/- 1.65, n = 15), and initial distal tubule (DCTa = 24.31 +/- 2.70, n = 6). It was minimal in the proximal tubule (PCT2 = 9.14 +/- 1.61, n = 16) and in the cortical (CCT = 5.23 +/- 0.75, n = 18) and medullary (MCT = 5.52 +/- 1.10, n = 12) collecting ducts. At a high concentration, the profile of labeling was roughly similar except for a relative increase in labeling much more pronounced in collecting ducts (CCT = +373, MCT = +323%) than in the other structures (MD = -14, CAL = +66, DCTa = +49, PCT = +9%). Pulse-chase experiments do not show evidence for differences in turnover or degradation rates of RNA between segments, at least in the PCT and the connecting part of the CCT. Analysis of the results at low and high concentration suggests that the observed heterogeneity in uridine labeling depends on both variable endogenous nucleoside pools and different rates of uridine incorporation into RNA from one segment to another.

Animals↗
PubMed1982

[Comparative study of light and electron microscopic aspects of myocardial lesions induced in the rat by oils with several amounts of gamma-linolenic acid (author's transl)].

Four different oils : onager oil, mixture of onager oil (20 p. cent) and middle class triglyceride, sunflower oil and soybean oil were given to the rat during three different periods of time : 1) during 3 months after weaning ; 2) during 6 months after weaning ; 3) from the 9th to 15th months. Three types of myocardial lesions were found : interstitial inflammatory reaction, foci of cellular necrosis and fibrosis. The semi-quantitative study of these lesions did not find important differences from one oil to the other, as well as with the controls. However, subgroups onager are among the least altered, particularly as far as necrotic lesions are concerned. Morphometry of mitochondria, despite evident alterations under electron microscope, gave similar results in every groups.

Animals↗
PubMed1981

[Cardiomegaly due to iron deficiency in the rat (author's transl)].

The effects of chronic iron deficiency were studied in new born rats. Hemoglobin concentrations were significantly depressed throughout the experimental period when compared with those of control animals. At third postnatal month, there was a 56% increase of absolute heart weight and a 230% increase of relative heart weight. Heart weight increased much more than myocytes dimensions. This finding may be interpreted as characteristic of a double process of hypertrophy and hyperplasia. Both cell hypertrophy and multiplication were responsible for the observed hypertrophy of the heart. Number of capillaries/mm2 was unchanged, but both mean capillary diameter and total surface of capillary wall increased progressively during the period of anemia. The adaptation to myocardial hypoxia may be, in this experiment situation, localized at the capillary level rather than at the mitochondria. At cell level, we did not note any particular modification of the ultrastructure and particularly no degenerative change. No mitochondrial lesions were found even in severe anemics (blood hemoglobin below 3.2 g/100 ml).

Anemia, Hypochromic↗
PubMedDate not supplied

Degree of granulation of atrial cardiocytes: its decrease after aorto-caval fistula in the rat.

The experimental creation of an aorto-caval fistula in the rat leads to a decrease of the degree of granulation of atrial cardiocytes. This decrease is significant for granules distal to the nucleus and not for paranuclear granules. This partial degranulation reinforces the hypothesis according to which atrial cardiocytes participate in some sensitivity to local changes in blood pressure and flow.

Aorta, Abdominal↗
PubMedDate not supplied

Aorto-caval fistula in the rat. An experimental model of heart volume overloading.

Abdominal aorto-caval fistula was experimentally induced in rats, and animals were sacrificed 5 days, 1 month, and 6 months later. Cardiac weight almost doubled the normal weight during the first month. Cell length increased to the same extent as the cell width, indicating harmonious growth. Increase in cell volume, as well as the amount of degenerative changes, were more pronounced in subendocardium than in midwall and subepicardium. Cytological features of active cellular growth was found not only in the early stages (5 days and 1 month) but also 6 months after the operation. This finding is consonant with proteosynthesis stimulation not only in the early period of constitution of hypertrophy but also in the later stages.

Animals↗