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Biomedical subjects

F Conrad

Publications and source records attributed to F Conrad.

14 recordsLinked to original sources
PubMed1991

Endotoxin effects on transmesothelial transport and intracellular uptake of albumin.

With a 2.9-mM concentration of unlabelled bovine serum albumin (BSA), the FITC-albumin transport (2 mg included) across the omental monolayer (0.48 +/- 0.16 mg/ml/30 min) was found to be significantly reduced as compared with the interstitial BSA concentration (290 microM) as it is the case, e.g., in peritonitis (0.79 +/- 0.09 mg/ml/30 min). Adding 10 micrograms lipopolysaccharide (LPS)/ml from Escherichia coli, serotype 0128:B12, we did not see any differences from the control. Cultured mesothelial cells took up double the amount of FITC-albumin (4.2 +/- 0.13 micrograms/10(5) cells/30 min) and in the presence of LPS the uptake of FITC-albumin was reduced to half the control (2.15 +/- 0.47 micrograms/10(5) cells/30 min). The results reveal the active participation of the mesothelium because high concentrations of BSA reduced exocytosis and stimulated endocytosis. Applying 10 micrograms/ml of LPS turned out to influence endocytosis and to reduce it at a high BSA concentration.

Animals↗
PubMed1989

[Tumor markers in the upper intestinal tract--stomach and pancreas].

Expectations of early diagnosis of carcinoma of the stomach and pancreas by using the tumour markers CEA and CA 19-9 have been disappointed. Although the sensitivity of CA 19-9 for pancreatic cancer is high, its field of application for screening is not efficient due to poor specificity of the marker. CEA and CA 19-9 are of great importance in the follow-up of pancreatic cancer. Results of tests of CEA and CA 19-9 in 100 patients with gastric cancer and 50 patients suffering from pancreatic cancer are reported.

Antigens, Tumor-Associated, Carbohydrate↗
PubMed1987

[New therapeutic aspects in the treatment of diffuse peritonitis].

The diffuse peritonitis is a syndrome during the course of which biochemical reactive chains are activated according to a cascade principle. Most of these biochemical reactive chains develop within an acid environment. The solutions applied for abdominal lavage so far had neutral pH value. The present acute peritonitis experiment has shown that a peritoneal pH increase during lavage therapy stopped the septic reactive chain and thus improved significantly the rate of survival in the test animals. These experimental findings ar now subject for clinical examination.

Animals↗
PubMed1975

An automated, direct method for measuring adipocyte cell size.

Current methods for direct determination of fat cell size are optical and non-automated. They are thus tedious, but have the advantage of providing estimates of the variance of a cell size distribution, as well as a measure of the mean cell size. An indirect method based on counting the number of osmium-fixed fat cells derived from a known wet weight of adipose tissue is also available. This method is automated and thus rapid, but does not provide information about the variance of the cell size distribution. In the present paper we describe a direct, automated method of fat cell sizing that provides estimates for both mean cell size and the variance of the cell size distribution. The correlation between our method and in indirect method based on counting osmium-fixed cells was 0.96. Transformation of the volume distribution to diameters indicated that cell diameters appeared to be normally distributed, confirming the observations of others.

Adipose Tissue↗
PubMedDate not supplied

[Age-related and pathophysiologic significance of capillary bed rarefaction of the hypertensive vascular system].

In spontaneously hypertensive rats (SHR), in normotensive related controls (WKY), as well as in non-related controls (NR), the rarefaction of the capillary bed in the gracilis muscle has been studied. By comparing four different in vivo and in vitro techniques for estimation of the number of capillaries, a rarefaction of about 30% in SHR, was found. The precapillary arterioles revealed a reduced rarefaction by about 25.5% or 40.2% in hypertensive, as compared with normotensive control animals. Age dependency was studied in animals of 6, 12, 24 and 48 weeks with respect to the rarefaction of the capillary bed. An age-dependent reduction of capillaries was seen from 385 to 272 cap/mm2 in NR, from 346 to 276 cap/mm2 in WKY, and an age- as well as hypertension-related rarefaction was found from 285 to 213 cap/mm2 in SHR. Under antihypertensive therapy (nifedipin, 300 mg/kg b.w.) systemic pressure was reduced significantly in all three strains studied and the capillary number changed from 674 to 1051 cap/mm2 in SHR, from 806 to 864 in WKY, and from 779 to 1019 in NR. The results indicate an age-dependency of the capillary rarefaction, which may be protracted and amplified by the hypertension disease. The pathophysiological meaning may be seen in reduction of the capillary surface area during a constantly elevated effective filtration pressure. By that, the increased transvascular fluid permeability of the capillary bed is prevented, which otherwise would cause edema formation in the hypertensive system.

Age Factors↗