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Biomedical subjects

F D Lublin

Publications and source records attributed to F D Lublin.

81 records · Page 5Linked to original sources
PubMed1984

Role of myelin antigens in murine relapsing experimental allergic encephalomyelitis.

Delayed, relapsing experimental allergic encephalomyelitis (DR-EAE) has been shown to be a good model of multiple sclerosis. This disorder has been produced in mice by immunization with whole central nervous system (CNS) tissue and adjuvants. The ability of various myelin components to produce DR-EAE was studied. Guinea pig myelin basic protein was able to induce DR-EAE that was clinically and pathologically identical to disease produced by whole CNS. Both acute inflammatory and chronic demyelinating lesions were seen in the CNS. Allogeneic mouse myelin basic protein either alone or in combination with cerebrosides and/or gangliosides was not able to produce DR-EAE.

Animals↗
PubMed1981

Syringoencephalomyelia (syringocephalus).

This is the fourth recorded patient with extension of syringomyelia into the brain rostral to the mesencephalon verified at autopsy. A syrinx was demonstrated from the low thoracic segments of the spinal cord to the cervico-medullary junction, where a fibrovascular malformation and dural-arachnoid adhesions deformed the pyramids. Cavities in the spinal cord were continuous, with rostral glial-lined cavities in both corticospinal tracts through a system of sponge-like tubes. The rostral extent of these cavities on the right was the centrum semi-ovale above the neostriatum; on the left, the cavities extended to the diencephalon. A classical lateral-dorsal syringobulbia in the right medulla accompanied the syringoencephalomyelia (syringocephalus).

Adult↗
PubMed1981

Delayed, relapsing experimental allergic encephalomyelitis in mice.

Immunization of female SJL mice with an emulsion of lyophilized mouse spinal cord, pertussis vaccine, and complete Freund's adjuvant produces a delayed and often relapsing form of experimental allergic encephalomyelitis (DR-EAE). The mice develop initial signs of disease an average of 6 mo after immunization. Relapses occurred 2 wk to 11 mo after the initial illness. Some animals had multiple relapses. Pathologic examination of the brain and spinal cord revealed perivascular infiltration of mononuclear cells with acute demyelination. Areas of subacute and chronic demyelination ("plaques") were also seen. This model produces a clinical course of relapsing-remitting disease with pathologic evidence of both recent and old inflammatory lesions at various levels of the central nervous system. It thus more closely resembles multiple sclerosis than acute EAE.

Animals↗
PubMed1980

Separation and properties of lymphocytes obtained from the central nervous system.

To study immune responses in inflammatory diseases of the central nervous system (CNS) we have devised a method of removing lymphocytes from brain and spinal cord. Experimental allergic encephalomyelitis was induced in adult Hartley guinea pigs to produce CNS inflammation. Brain and cervical spinal cord were removed and minced. The single-cell suspension was centrifuged on a modified Ficoll-Hypaque gradient. Lymphocytes passed through to the pellet, while brain cells stayed at the top of the gradient. An average of 1.7 x 10(6) lymphocytes was obtained from a single guinea pig brain and cervical spinal cord. When the CNS lymphocytes were cultured in the presence of phytohemagglutinin (PHA) or purified protein derivative (PPD) proliferative responses were obtained. No proliferative responses occurred when CNS lymphocytes were cultured with myelin basic protein. Using this technique, viable lymphocytes can be separated from inflamed CNS and can be cultured in vitro to measure cell-mediated immune responses.

Animals↗
PubMed1979

Myokymia and impaired muscular relaxation with continuous motor unit activity.

We have studied two cases of the syndrome of myokymia and impaired muscular relaxation with continuous motor unit activity. Both patients complained of muscle twitching, weakness, stiffness, and hyperhydrosis during their illness. Myokymia was present over the entire body in both. On repetitive testing of muscle strength each patient showed initial fatigue followed by increasing strength as he continued his efforts. Both patinets improved on phenytoin therapy at high blood levels. Nerve conduction velocities were decreased. Electromyograms showed continuous electrical activity at rest which persisted during sleep and spinal anaesthesia but was diminished by curare. Intravital staining with methylene blue in one case demonstrated sprouting and beading of motor nerve terminals with multiple innervation of muscle fibres. The neurophysiological and pathological findings in these two cases indicate an abnormality of peripheral nerve in this disorder.

Adult↗
PubMedDate not supplied

Delayed, relapsing experimental allergic encephalomyelitis in mice. Role of adjuvants and pertussis vaccine.

The effect was studied of varying the dosage of mycobacteria and pertussis vaccine in the immunization emulsion on the development of delayed, relapsing experimental allergic encephalomyelitis (DR-EAE) in SJL/J mice. DR-EAE could be induced with dosages of mycobacteria from 0.2 mg to 1.0 mg per mouse. Animals given pertussis vaccine from different batches and different sources all developed DR-EAE with similar percentages of animals sick, animals relapsing, and delay to onset. Mice immunized without pertussis vaccine also developed DR-EAE but had fewer relapses and a longer delay to onset. The role of antigens and adjuvants in relapsing EAE and analogies with multiple sclerosis are discussed.

Animals↗