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Biomedical subjects

F De Rosa

Publications and source records attributed to F De Rosa.

At least 19 recordsLinked to original sources
PubMed1992

[Diltiazem in paroxysmal supraventricular tachycardia: electrocardiographic findings at termination].

The effects of diltiazem hydrochloride (0.3 mg/kg i.v. over 2 min.) was studied by continuous electrocardiographic monitoring in 60 patients. Conversion to sinus rhythm was achieved in 55 patients (91%). Electrocardiographic findings were: undisturbed sinus rhythm in 20 patients; A-V junctional rhythm in 4 patients; complex ventricular arrhythmias (ventricular tachycardia or complex VPCs) in 19 patients. Patients with complex ventricular arrhythmias were matched against patients with normal sinus rhythm, with respect to the following parameters: age, sex, heart disease, tachycardia duration, tachycardia cycle length, sinus cycle length, pre- and post-infusion blood pressure. No differences between the two groups of patients were found. Ventricular arrhythmias occurring at the termination of supraventricular tachycardia are difficult to explain. Nevertheless, these arrhythmias are not associated with organic heart disease. They could be the expression of triggered activity.

Adolescent↗
PubMed1991

The serological diagnosis of human hydatid disease by time-resolved fluoroimmunoassay.

The use of a new immunoassay, time-resolved fluoroimmunoassay (TR-FIA), in the diagnosis of human hydatid disease has been evaluated. This technique, which is based on the labelling of antibodies with europium (Eu), was compared with a well-established method, the enzyme linked immunosorbent assay (ELISA). Of 102 patients with hydatid disease, 97 (95.1%) were positive according to TR-FIA and 83 (81.4%) according to ELISA. The rate of non-specificity for other parasitic infections (n = 206) was 8.7% for TR-FIA and 17.5% for ELISA. It is concluded that TR-FIA is more sensitive and more specific than ELISA in the diagnosis of human hydatid disease.

Animals↗
PubMed1990

Treatment of Echinococcus granulosus hydatid disease with albendazole.

The authors report their personal experience of the treatment of 50 patients with hydatid cysts of different localization with 10-12 mg Albendazole per kg per day for three months without intervals. During the treatment patients were submitted to careful clinical, biochemical, radiological and immunological controls. In all 105 hydatid cysts were observed, and the follow-up periods ranged from six to 42 months. The side effects were not severe. Four patients were considered healed, 31 improved, and 11 showed no change. Three patients relapsed after the end of the therapy, and were treated with a further cycle of Albendazole at the same dose rates, with good effect. The observed results are encouraging, and most of the patients did not require surgery.

Adolescent↗
PubMed1990

Basophil releasability in human hydatidosis.

We studied immunoglobulin E (IgE)- and non-IgE-mediated releasability in basophils from 31 patients with hydatidosis. Histamine release to non-IgE-dependent stimuli did not differ significantly between normal individuals and patients with hydatidosis. On the contrary, an increased histamine liberation was obtained by challenging basophils from hydatid patients with anti-human IgE. It is concluded that Echinococcus granulosus infection induces an enhanced sensitivity of basophils to IgE-dependent stimuli.

Animals↗
PubMed1990

[Ventricular arrhythmias in the acute phase of myocardial infarct and in the postinfarct. A 1-year follow-up].

A total of 90 AMI patients (80 male, 10 female; mean age: 62 years, range: 36-70 yrs) who were admitted to the coronary care unit within six hours of the onset of symptoms were evaluated for the following: the incidence and variability of complex ventricular ectopic beats (classes Lown III-V) in the acute, subacute and chronic phases of myocardial infarction. The diagnostic utility of Holter monitoring and treadmill exercise testing was compared so as to reveal complex ventricular ectopic beats. The relation between complex ventricular ectopic beats and left ventricular dysfunction was determined using the echocardiographic technique (ejection fraction, fractional shortening). The hospital mortality rate was 6.6% (6/9 patients) while the post-hospital mortality rate was 3.5% (3/84 patients). The prevalence rate of complex ventricular ectopic beats detected by Holter monitoring was respectively: 85.5% (77/90 patients) in the acute phase, 8.5% (7/80) at the 72nd hour, 18.5% (15/80) in the pre-discharge phase. At 1, 3, 6 and 12 months the prevalence rate was respectively 35% (27/77 patients), 28.8% (22/77), 24.6 (19/77), 24.6% (19/77). A high variability in arrhythmic groups was found. Each group was composed of new arrhythmic patients and stable arrhythmic patients, in addition to a small number of patients who were arrhythmic in the preceding control but no arrhythmic in the following control. This specific analysis of various groups revealed that the pre-discharge phase is the period with the highest number of "stable arrhythmic" patients (4 Holter positive out of 5) compared to the 1st month (3 Holter positive out of 4) and the 3rd month (2 Holter positive out of 3).(ABSTRACT TRUNCATED AT 250 WORDS)

Adult↗
PubMed1989

[Critical considerations on various experimental staphylococcus infections].

The authors discuss some problems concerning the use of models in biomedical research and particular animal models of staphylococcal infections simple, reproducible and similar to the corresponding human diseases. Two models of staphylococcal infections are examined in particular: experimental endocarditis and osteomyelitis, considering their characteristics and reliability in relation to the corresponding human diseases.

Animals↗
PubMed1989

Hydatid disease: analysis of parasite antigens in circulating immune complexes and in preformed hydatid antigen-antibody complexes.

Fifty-three sera from 29 patients with hydatid disease, all but one positive for specific anti-parasite antibodies and all negative for specific circulating antigens, were studied for the presence of circulating immune complexes (CIC) by conglutinin binding-assay (KgBA). Fourteen serum samples (26%) from eight patients (27%) were positive. These positive sera were pooled for each patient and the eight samples were PEG-precipitated and analysed for the presence of specific Echinococcus granulosus antigens in the CIC using a human anti-human-hydatid cyst fluid antiserum capable of recognizing the major antigenic systems of the parasite namely, antigens 4 and 5. The assays utilized for detecting antigen in CIC were: (a) blotting on nitrocellulose paper after sodium dodecil sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and specific immunological detection; (b) ultracentrifugation in acid buffer and subsequent detection of antigens by a sandwich-radioimmuno assay (RIA); (c) protein separation by isoelectric focusing (IEF) and specific immunological recognition. In addition, all positive sera were analysed for the presence of antigen in the CIC by a modified KgBA and by polyethylenglicol (PEG)-precipitation in acid buffer followed by immunological recognition of antigen. All tests gave negative results with the patients' samples, but were positive with preformed in vitro complexes between parasite antigens and corresponding antibodies. Failure to detect antigen in the CIC could be due to: 1) insufficient sensitivity of the assays used to detect hydatid antigens in CIC; 2) rapid clearance of antigen or CIC from the circulation; 3) presence of parasite antigen not recognized by the antiserum employed; 4) production of CIC as a result of polyclonal B-cell activation. This last hypothesis is supported by the demonstration of IgM-rheumatoid factor (RF) and anti-F(ab')2 antibodies respectively in 11 (44%) and 13 (52%) out of 25 patients.

Animals↗
PubMed1989

Treatment of Echinococcus granulosus hydatid disease with mebendazole.

The Authors report their personal experience in the treatment of 70 patients with hydatid cysts of different localization with mebendazole, following protocols approved by WHO. During treatment patients were submitted to careful clinical, biochemical, radiological and immunological controls. On the whole, 150 hydatid cysts and 13 cases of widespread hydatidosis were observed. Follow-up ranged from 6 months to 5 years. Morphological and/or volumetric modifications were observed in 64.3% of the total number of cysts treated, regardless of their localization. 9 relapses were observed, and all but one case showed the same previous sensitivity to a further cycle of mebendazole. The observed side effects were not severe.

Adolescent↗
PubMed1989

Histamine release test in the diagnosis of human hydatidosis.

An evaluation of the use of the histamine release test (HRT) in the diagnosis of human hydatidosis is presented. This technique, which makes it possible to evaluate directly IgE dependent immediate hypersensitivity by measuring the histamine released from leucocytes (basophils) after antigenic challenge, was compared with the detection of serum specific IgE by the radio-allergosorbent test (RAST), and with the determination of serum specific IgE by the enzyme-linked immunosorbent assay (ELISA). Of the 54 patients with hydatidosis, all were positive according to HRT, 42 according to RAST and 47 according to ELISA. No false positive results were obtained by HRT in 43 patients with parasitosis other than hydatidosis, however, of these 43, 10 resulted in false positives according to RAST and five according to ELISA. It is concluded that HRT is more sensitive and more specific than RAST and ELISA.

Basophils↗
PubMed1989

[A case of disseminated mucormycosis].

Report of a case of disseminated mucormycosis. The Authors, after a review of the literature, report a fatal case of disseminated Mucormycosis observed in a young patient with aplastic anemia, severe neutropenia and treated with Deferoxamine.

Adult↗
PubMed1989

Spectrotypic analysis of humoral response in human hydatidosis.

The spectrotypic patterns of anti-Echinococcus granulosus and anti-F(ab')2 antibodies were analysed in 13 patients with different forms of hydatidosis by means of isoelectric focusing (IEF). Specific anti-echinococcus antibodies and specific echinococcus antigens were obtained by crossed affinity chromatography between patient serum and cyst fluid, as previously described (D'Amelio et al., 1985). A wide spectrotype in the pattern of anti-Echinococcus antibodies was observed, with strong homologies among the different sera mainly at acid and neutral pH. One serum displayed faint, nearly inapparent bands. Conversely, the pattern of anti-F(ab')2 antibodies in the six patients in whom they were positive was limited but heterogeneous, ranging from monoclonal through oligoclonal to polyclonal. A predominant clone was identified in the patient with a polyclonal pattern. A correlation between qualitative IEF spectrotypic patterns and the quantitative results of immunoenzymatic assays was observed for both types of antibodies.

Adolescent↗
PubMed1988

Primary connections of the anterior and posterior lateral line nerves in the oyster toadfish.

This study used anterograde transport of horseradish peroxidase to identify primary projections of the anterior and posterior lateral line nerves in the oyster toadfish, Opsanus tau. Both lateralis nerves project to the nucleus medialis and nucleus caudalis, which comprise a continuous lateralis cell column. Separation between nucleus medialis and nucleus caudalis is based on cell type and position. Unlike the primitive condition, the nuclei are not obviously separable on the basis of gross topography. The anterior lateral line nerve projects to the ventral area, and the posterior lateral line nerve projects to the dorsal area of the lateralis cell column. Anterior lateral line nerve projections to nucleus medialis terminate in a larger area than do projections from the posterior lateral line nerve, suggesting that more lateralis information enters from the head than from the trunk and tail. This speculation is reasonable considering the animal's large head and sedentary bottom existence. Both lateral line nerves project to the nucleus magnocellularis and the eminentia granularis, but only projections from the posterior lateral line nerve were seen in the granular layer of the cerebellum.

Afferent Pathways↗
PubMed1988

Cellular immune responses of hydatid patients to Echinococcus granulosus antigens.

The reactivity of peripheral blood mononuclear cells (PBMC) from 40 hydatid patients to hydatid fluid (HF) and to two hydatid fractions (pH5PPT and pH5SUP) was evaluated by the incorporation of 3H-methylthymidine into DNA. Maximal responses were detected using 200 micrograms/ml protein after 7-9 days incubation. The three antigen preparations were inducers of PBMC proliferation, with a good correlation (r2 = 0.87) of the responses induced by HF and by the fraction pH5PPT, which contains the two major hydatid antigens (5 and B). Lymphocytes from healthy donors and non-hydatid patients showed no response to these antigens. Neither direct nor inverse correlation was found between the results of the serological tests and of the PBMC proliferation assays. The majority of the patients (75%) responded in serological and in cellular tests. Of the remaining patients, six showed high antibody response associated with a negative PBMC proliferation assay and conversely four seronegative patients were found to respond positively in the PBMC proliferation assay. No relationship of the pattern of immune responsiveness to the patient's clinical forms could be established. Use of the PBMC proliferation assay with hydatid antigens appears rational in those patients which are low antibody producers, but the test is still not to be considered applicable for routine diagnosis.

Animals↗