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F DeVenuto

Publications and source records attributed to F DeVenuto.

32 records · Page 2Linked to original sources
PubMed1978

Stability of hemoglobin solution during extended storage.

Hemoglobin solution, proposed as a blood substitute, must be stable for a long period of time in order to be stockpiled and be readily available when massive clinical transfusions are required. Hemoglobin solutions, prepared by crystallization and maintained in blood bags under sterile conditions, were stored at -20 degrees, 4 degrees, and 25 degrees C. At several intervals, methemoglobin content, p50, n values, osmolality, oxygen capacity, Na, K, and pH were assayed. Solutions maintained at -20 degrees C demonstrated no alterations in these parameters after 2 years of storage. Solutions kept at 4 degrees C remained stable for 12 months. After 12 months and especially after 18 months, deterioration was evident inthe refrigerator-stored solutions, as demonstrated by an increase in methemoglobin content and a decrease in P50. Attempts to store hemoglobin in liquid form at room temperature without alterations in structure and function of hemoglobin were unsuccessful. The characteristics of hemoglobin prepared from fresh or outdated blood were essentially the same.

Cold Temperature↗
PubMedDate not supplied

Distribution of progesterone and its effect on human blood during storage.

Human blood, drawn into ACD, was stored under blood bank conditions with and without addition of tritium-labeled progesterone. At selected storage intervals, several biochemical and biophysical parameters were assayed and the distribution of the hormone between plasma and erythrocytes was determined. The retention of the radioactive material by erythrocytes was studied by repeated washes and isotonic saline. The results indicate that erythrocytes stored with addition of progesterone have higher ATP levels, less spontaneous lysis, higher osmotic resistance, and higher uptake of methylene blue throughout the time of storage than do cells stored without added progesterone. This hormone appears to be in steady-state equilibrium between plasma and red blood cells during 42 days of storage and the amount in the plasma is almost twice that present in the cells. After repeated saline washes, about 10 er cent of the progesterone remains attached to the erythrocytes. Specific association of progesterone with red blood cell populations of different density shows that high specific activity is obtained in cell populations of low density (young cells) throughout the storage time investigated.

Adenosine Triphosphate↗