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F Escande

Publications and source records attributed to F Escande.

29 records · Page 2Linked to original sources
PubMed1993

Characterization of Pasteurella from gingival scrapings of dogs and cats.

Gingival scrapings of 62 dogs and cats were examined for the presence of Pasteurella. Isolation was performed in a medium supplemented with thiostrepton. Twenty-eight and 37 strains were obtained from 21 dogs and 26 cats, respectively, and classified in recently described species or subspecies of the genus Pasteurella (P.): P. multocida subspecies multocida and septica, P. canis, P. dagmatis and P. stomatis. Twenty-one strains were classified as atypical P. stomatis and one strain obtained from a cat remained unclassified. All strains were susceptible to the antibiotics studied. P. multocida and P. stomatis (including atypical strains) represented 65 and 30% of feline isolates, and 14 and 68% of canine isolates, respectively. Assuming that P. multocida, P. canis and P. dagmatis are potentially pathogenic for humans, and that P. stomatis has a low pathogenicity or non-pathogenic, 77 and 28% of examined cats and dogs harboured one or several pathogenic strains. This difference could explain the fact that Pasteurella infections in man are lower in dog bites rather than cat bites.

Agar↗
PubMed1993

Detection of an adenylate cyclase gene in Pasteurella species.

A Pasteurella multocida adenylate cyclase gene has been previously cloned in Escherichia coli and sequenced. A 1200 bp HpaI fragment from the coding region was used as a probe to analyse the presence of the gene in different Pasteurella species and subspecies, Actinobacillus ureae (formerly P. ureae) and group EF-4 bacteria. Thirty-seven strains were checked for the presence of the gene. It was shown that the adenylate cyclase gene was detected only in the species Pasteurella multocida.

Adenylyl Cyclases↗
PubMed1993

Epidemiology of human infections by Pasteurella and related groups in France.

A retrospective study of infections due to Pasteurella (P.) and related groups was performed between the Pasteurella National Center and Nancy's hospital from 1985 to 1991. Among the 958 cases recorded, wound infections (bites, scratches and punctures) were the common forms of pasteurellosis (66%) caused by P. multocida (48%), P. canis (11%), P. dagmatis (5%), P. stomatis (4%), and in few cases by groups EF-4 and M-5 (14 and 13%, respectively). In human infections unrelated to animal wounds, respiratory tract diseases and bacteremia-septicemia were the predominant infections with respectively 19 and 11%, and caused by P. multocida. Next in importance were urogenital (2.5%), abdominal (1%) and central nervous system (< 1%) infections. The majority of animal bite wound infections was treated with penicillins or tetracyclines; with other forms, penicillins and cephalosporins were more likely.

Adult↗
PubMed1991

Resistance to trimethoprim and 2,4-diamino-6,7-diisopropyl-pteridine (0/129) in Pasteurella haemolytica.

Thirteen strains of Pasteurella haemolytica resistant to moderate levels of trimethoprim (MICs from 8 to 64 micrograms/ml) and 0/129 (MICs from 16 to 64 micrograms/ml) were isolated from bovine specimens. Two strains, CNP330 and CNP334, were studied and found to harbour various plasmids but all attempts to cure trimethoprim resistance were unsuccessful. Resistance characters were not transferable to Escherichia coli or to Pasteurella multocida by conjugation and to E. coli by transformation. The resistance gene(s) was therefore tentatively assigned to a chromosomal location and cloned into E. coli where it conferred trimethoprim resistance. Trans-complementation analysis of a dihydrofolate reductase-deficient mutant of E. coli showed that trimethoprim resistance was secondary to synthesis of a dihydrofolate reductase. DNA/DNA hybridization of the hybrid plasmid and of strains CNP330 and CNP334 with probes specific for dihydrofolate reductase types I to V were negative, indicating that cross-resistance to trimethoprim and 0/129 in P. haemolytica was due to the acquisition by P. haemolytica of a new resistance determinant.

Animals↗
PubMed1987

[In vitro antibacterial activity of ceftizoxime on Pasteurella and EF4].

The in vitro activity of ceftizoxime, a new third-generation cephalosporin, was tested by determining minimal inhibitory concentrations (MIC) by agar dilution method, for 150 strains of genus Pasteurella and group EF4 bacteria from various sources. All the strains were susceptible to ceftizoxime and inhibited by 0.03 micrograms/ml concentration. No significant difference appeared between the 7 species and human and animal strains. Group EF4 bacteria, frequently isolated from animal bite wounds in humans, had higher MIC (O.25-1 micrograms/ml). Results were compared to those obtained with cefotaxime, cephalothin, penicillin G and ampicillin.

Cefotaxime↗
PubMedDate not supplied

Biological characterization of Actinobacillus species and Pasteurella ureae.

Forty-seven strains of Actinobacillus and eleven strains of Pasteurella urea were studied using 119 morphological, physiological and biochemical characters. The resulting data were subjected to numerical analysis using the complement of Gower's coefficient excluding negative matches. Clustering was by unweighted pair group average linkage. At distance level 0.30, seven phenons and five isolated strains (including one strain of "A. seminis ") were obtained. The seven phenons correspond to Actinobacillus lignieresii , A. suis, A. equuli , A. capsulatus, "A. salpingitidis ", Actinobacillus sp. (Ross) and P. ureae. The characteristics allowing identification of the seven phenons are tabulated.

Actinobacillus↗