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F F Badakhsh

Publications and source records attributed to F F Badakhsh.

7 recordsLinked to original sources
PubMed1982

Improved rapid slide agglutination test for presumptive diagnosis of canine brucellosis.

A modified rapid slide agglutination test for the presumptive identification of Brucella canis infection in dogs has been developed. The method required mixing 0.1 ml of canine serum with 0.1 ml of 0.2 M 2-mercaptoethanol solution. Equal volumes (0.05 ml) of the treated serum and the B. canis plate antigen were mixed. Agglutination results were read within 2 min. Clinical studies showed 100% agreement between this method and the conventional 2-mercaptoethanol tube agglutination test. Excellent correlation was shown between cultural isolation and the modified rapid slide agglutination test, using sera from experimentally infected dogs.

Agglutination Tests↗
PubMed1970

Comparison of Chemical Components of Cell Walls of Brucella abortus Strains of Low and High Virulence.

Amino acid, carbohydrate, and lipid components of cell walls of Brucella abortus strain 19A (low virulence) and strain 2308 (high virulence) were compared by thin layer chromatography (TLC) and by use of an amino acid analyzer. A total of 15 amino acids were detected by both chromatographic methods. Each amino acid was present in greater amounts in strain 2308 than in strain 19A when equal amounts of hydrolysates of cell wall and endotoxin-containing preparations were analyzed. A component with the same R(F) value as ethanolamine was present in strain 2308 cell wall hydrolysates but was not revealed by TLC of strain 19A cell wall hydrolysates. This component was not detected with the amino acid analyzer. TLC of cell walls tagged with 2,4-dinitrofluorobenzene prior to hydrolysis showed that phenylalanine was a terminal amino acid in cell walls of B. abortus strains 19A and 2308, B. suis strain 1776, and B. melitensis strain 2500. Carbohydrates detected in cell walls of strains 19A and 2308 by TLC were tentatively identified as glucose, mannose, rhamnose, and galactose. Colorimetric tests were also positive for 2-keto-3-deoxy-octulosonic acid, heptose, and dideoxyhexose. At least seven lipid components were detected by TLC of ether extracts of cell walls of strains 19A and 2308. It is suggested that one or more lipids is important in maintaining cell wall structure, because isolated cell walls rapidly became fragmented after exposure to ether.

Journal Article↗
PubMed1969

Deoxycholate-treated, nontoxic, whole-cell vaccine protective against experimental salmonellosis of mice.

A vaccine prepared from the residue after extraction of whole cells of Salmonella typhimurium with 2% sodium deoxycholate proved to be nontoxic and highly immunogenic. The material was not lethal for mice at 6.0 mg and was essentially nontoxic in rabbit skin, whereas endotoxic activity was found in the dialyzed extract. A high dosage, above 2.0 mg, was less protective than lower doses, indicating a degree of "immunologic paralysis." Three inoculations of low doses, 0.25 mg each, induced protection against death and tissue infection in animals challenged with 2,000 ld(50) of virulent homologous S. typhimurium and against death, but not against tissue infection, after heterologous challenge with S. enteritidis. Residues of purified cell walls were as effective as residues of whole cells, indicating that the immunizing antigen(s) resided in the cell wall.

Animals↗
PubMed1966

Detoxification and immunogenic properties of endotoxin-containing precipitate of Brucella abortus.

Badakhsh, Fred F. (University of Georgia, Athens), and John W. Foster. Detoxification and immunogenic properties of endotoxin-containing precipitate of Brucella abortus. J. Bacteriol. 91:494-498. 1966.-Endotoxin-containing precipitates (ECP) were prepared from Brucella abortus strain 19A by aqueous ether extraction followed by ethyl alcohol precipitation. Lysozyme was the most effective of several enzymes tried for detoxification of endotoxin present in the precipitate. Trypsin was shown to reduce mouse lethal toxicity but not rabbit dermal toxicity. Immunological studies of ECP and enzyme-treated ECP demonstrated that lysozyme did not harm the immunogenic property of ECP, whereas heat, ribonuclease, lipase, and proteolytic enzymes had an adverse effect. Serological reactivity of ECP was increased after lysozyme treatment, whereas ribonuclease reduced serological activity.

Animals↗