Determination of semivolatile compounds in drinking water by tandem mass spectrometric detection.
Explore the source record for details and available documents.
China↗
Biomedical subjects
F Feng
Publications and source records attributed to F Feng.
Autologous transplantation of gingival fibroblast-like cells and a hydroxylapatite complex graft in the treatment of periodontal osseous defects: cell cultivation and long-term report of cases.
Autogenous cell transplantation via hydroxylapatite (HA) vehicle has been reported to have beneficial effects on the treatment of human periodontal osseous defects. The aim of this study was to explore the possibility of using gingival fibroblast-like cells in the therapy of osseous defects caused by inflammatory periodontitis by reporting long-term results of gingival fibroblast-coated hydroxylapatite (GF-HA) grafting for healing these defects. Gingival fibroblasts were cultured from healthy gingivae of treated subjects. Growth of cells on HA particles was established in vitro, and then the GF-HA complex was transplanted into the periodontal osseous defects. Clinical parameters of gingival and plaque indices, probing depth, and periapical x-ray were monitored at baseline and at various periods from 50 months to 6 years after surgery. Grafting with only HA in the osseous defects of the same patient was used for comparison. The present study shows that GF-HA-treated sites could achieve marked pocket reduction and probing attachment gain at reentry and later recalls. Good clinical bone filling of osseous defects in GF-HA-treated sites was also demonstrated in periapical radiographs (increased bone height and reappearance of the crestal cortex) and in some reentry sites. One HA-treated site was filled with connective tissue only, and the absence of new bone formation was noted during a reentry operation. Another HA-treated site exhibited a comparable increase in radiographic density, while part of HA particles were gradually lost in longer recalls. These limited observations conclude that GF-HA grafting may provide a treatment modality leading to regeneration of periodontal tissues in periodontitis-affected osseous defects. Further studies including more cases and demonstration of the deposition of differentiated periodontal tissues are necessary before further application of this therapy.
Adult↗
Akt1/PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice.
The serine-threonine kinase Akt, also known as protein kinase B (PKB), is an important effector for phosphatidylinositol 3-kinase signaling initiated by numerous growth factors and hormones. Akt2/PKBbeta, one of three known mammalian isoforms of Akt/PKB, has been demonstrated recently to be required for at least some of the metabolic actions of insulin (Cho, H., Mu, J., Kim, J. K., Thorvaldsen, J. L., Chu, Q., Crenshaw, E. B., Kaestner, K. H., Bartolomei, M. S., Shulman, G. I., and Birnbaum, M. J. (2001) Science 292, 1728-1731). Here we show that mice deficient in another closely related isoform of the kinase, Akt1/PKBalpha, display a conspicuous impairment in organismal growth. Akt1(-/-) mice demonstrated defects in both fetal and postnatal growth, and these persisted into adulthood. However, in striking contrast to Akt2/PKBbeta null mice, Akt1/PKBalpha-deficient mice are normal with regard to glucose tolerance and insulin-stimulated disposal of blood glucose. Thus, the characterization of the Akt1 knockout mice and its comparison to the previously reported Akt2 deficiency phenotype reveals the non-redundant functions of Akt1 and Akt2 genes with respect to organismal growth and insulin-regulated glucose metabolism.
Alleles↗
[Diagnosis and treatment of pulmonary embolism during pregnancy].
OBJECTIVE: To study high risk factors, clinical presentation, diagnosis and treatment of pulmonary embolism (PE) during pregnancy and postpartum. METHODS: Two patients with pulmonary embolism were reported retrospectively. RESULTS: The 1st case was a pregnant woman with congenital heart disease at 39 weeks gestation, who underwent uneventful cesarean section (CS) because of heart disease, but she had tachycardia, tachypnea, cyanosis, dyspnea suddenly on the 10 days after CS and died immediately after the onset of above symptoms, the diagnosis of PE was highly suspected clinically. The 2nd case was a pregnant women with twin gestation, at 35 weeks, because of severe prenatal myocardiopathy, cesarean section was performed, Maternal death occurred suddenly during the surgery. The diagnosis of PE was confirmed by autopsy. CONCLUSIONS: The maternal mortality of PE during pregnancy and postpartum is very high, whenever there is any suspects, the objective examination for PE should be started early in order to get the chance to give anticoagulant therapy.
Adult↗
[Clinical study of topotecan in the treatment of small cell lung cancer and recurrent ovarian cancer].
OBJECTIVE: To evaluate the effect and adverse reaction of China made topotecan in the treatment of small-cell lung cancer (ACLC) and recurrent ovarian cancer (OV). METHODS: From January to July, 2000, topotecan was used to treat 141 patients at a dose of 1.2 mg/m2, given daily as 30-min i.v. infusion for 5 days. Treatment was repeated once every 3 weeks. Of the 141 patients, 118 were evaluable for therapeutic efficacy. All the patients received a total of 286 cycles of treatment were assessable for analysis of adverse reactions. RESULTS: Among the evaluable patients, there were 5 CR, 35 PR, with an overall response rate (RR) of 33.8%. There were 3 CR and 26 PR in 89 patients with SCLC (RR 32.5%). The response rate of patients with or without prior chemotherapy was 15.6% and 50%, respectively. In 29 patients with recurrent OV, there were 2 CR and 9 PR (RR 37.9%). The major toxic effect was myelosuppression. Non-hematopoietic toxicities were mild and tolerable. CONCLUSION: Topotecan is an effective drug for the treatment of SCLC and recurrent OV. It is still efficacious in some patients who previously received standard chemotherapy. The major dose-limiting toxicity is myelosuppression. The response rate and toxicity of the domestically made topotecan are comparable with those of the imported one.
Adolescent↗
[Modulation of human small cell lung cancer cell line GLC4/ADR multidrug resistance in the inhibition of multidrug resistance-associated protein and its antisense].
OBJECTIVE: To study the effect of antisense multidrug resistance-associated protein (MRP) RNA on multidrug resistance (MDR) in the human small cell lung cancer (SCLC) cell line GLC4/ADR, in which the overexpression of MRP gene is discovered. METHODS: In using the plasmid pRC/RSV-MRP1 containing complete ORF of MRP as a template, two antisense recombinants targeting at the 5' and 3' regions were constructed with the application of the polymerase chain reaction (PCR) technique. Lipofectamine was conducted to transduce these antisense MRPs into the GLC4/ADR cells. Three clones (the GLC4/ADR-pcDNA3, MO; GLC4/ADR-MRP-5' region, Ma; GLC4/ADR-MRP-3' region, Mb) of transfectants after the selection of G418 were obtained. The expression of MRP protein was detected by the use of the Western blot and the MTT method for determination of chemosensitivity to ADR was used. RESULTS: The antisense MRPs was found to be effectively expressed in the clones, being transfected with two different antisense MRPs. The MRP expression in these transfectants were inhibited at the rates of 14.0% (GLC4/ADR Ma) and 83.0% (GLC4/ADR Mb), respectively. Moreover, decrease in the ADR resistance was observed in the Ma and Mb at the rates of 9.5% and 28.4%. Although the intracellular ADR concentration was increased in these transfectants, the proliferation and cell cycle and their early apoptosis induced by the ADR, indicated no difference between the transfectants and parental cells. CONCLUSION: It is possible to obtain the effective expression of the MRP antisense structures for the blocking of the mRNA translation in GLC4/ADR cell line and the fragment complementary to the 3'-region of MRP is more effective for the inhibition of the MRP than that related to the 5'-region of MRP. The antisense RNA may be a useful treatment in combination with the conventional chemotherapy for SCLC, in which the MRP overexpression usually occurs.
ATP Binding Cassette Transporter, Subfamily B↗
[The relationship between cadmium-induced inhibition of splenic lymphocyte function and cell apoptosis].
OBJECTIVE: To explore the relationship between cadmium-induced inhibition of splenic lymphocyte function and cell apoptosis in vitro. METHODS: The splenic lymphocytes of mice were exposed to 3.10, 6.25, 12.50, 25.00, 50.00 micromol/L of cadmiun chloride (CdCl(2)) for various time period, to measure the lymphocyte transformation by MTT colorimetric assay and to detect the apoptosis in splenocytes by DNA agarose electrophoresis and flow cytometry (FACS). RESULTS: CdCl(2) could significantly inhibit the function of lymphocyte transformation in a dose-response pattern at concentrations of 25.00 and 50.00 micromol/L, with inhibition rates of 50% and 78% in ConA-stimulating group and 39% and 55% in LPS-stimulating group, respectively. CdCl(2) could also induce apoptosis of splenic cells at concentrations of 12.50 to 50.00 micromol/L. RESULTS: of FACS analysis showed 30% - 60% of cell apoptosis. Higher concentration of CdCl(2) could also cause reduction of cell survival. Effect of Cd-induced apoptosis was earlier and at a lower concentration of CdCl(2) than that of Cd-induced inhibition of lymphocyte transformation. CONCLUSION: CdCl(2) could induce cell apoptosis in vitro, which was one of the mechanisms of its suppression of lymphocyte function.
Animals↗
[Safety and immunogenicity of a new inactivated hepatitis A vaccine].
BACKGROUND: To evaluate the safety and immunogenicity of a new inactivated hepatitis A virus (HAV) vaccine. METHODS: In a randomized controlled trail thirty-one HAV-seronegative adults were enrolled and randomly assigned to either study group or control group. Subjects in the study group were given 1000 units of the new inactivated HAV vaccine, with a booster at 3 months. Subjects in the control group were given 720 ELISA units Havrix, produced by SmithKline Beecham Biologicals, with a subsequent dose at 3 months. Local and systematic reactions and serum response to the vaccines were compared between the two groups. RESULTS: Mild local reactions were noted in one subject from the study group and two from the control group after primary vaccination. Mild systematic reactions were reported in two subjects from each group after primary vaccination and in one subject from the study group after booster vaccination. Seroconversion rates were 94%, 100%, and 100% in the study group at 1, 3, and 4 (1 month after booster) month, respectively. The corresponding figures were 73%, 80% and 100% in the control group. Geometric mean antibody titers were 139.2 mIU/ml, 137.7 mIU/ml, and 1 066.7 mIU/ml at 1, 3, and 4 months,respectively, in the study group,and 104.3 mIU/ml, 111.3 mIU/ml, and 760.7 mIU/ml in the control group. CONCLUSIONS: The new inactivated hepatitis A vaccine was safe and highly immunogenic.
Adolescent↗
Mammary tumor development in MMTV-c-myc/MMTV-v-Ha-ras transgenic mice is unaffected by osteopontin deficiency.
Transgenic mice expressing c-myc and v-Ha-ras specifically in the mammary gland under the control of the mammary specific promoter MMTV develop unifocal mammary tumors with a half time of about 46 days, and these tumors express high levels of osteopontin mRNA and protein. In order to evaluate the requirement for osteopontin expression by these tumors, we have crossed transgenic mice expressing these two oncogenes with mice with a targeted disruption of the osteopontin gene. Littermates expressing both myc and ras, and with either wild-type or disrupted OPN alleles were evaluated for tumor incidence and growth rate. Both of these parameters were found to be unaffected by a lack of osteopontin in the whole animal. Ras and myc expression level, measured at the level of mRNA, was not different in tumors of the two genotypes. Macrophage accumulation, while extremely variable among different tumors, did not correlate with the OPN status of the animals. Expression of the related gene BSP was not detected in any of the tumors, and was similar in bones of wildtype and OPN -/- mice. Similarly, the vitronectin gene was expressed at very low levels in tumors of either genotype. These results indicate that despite its high level of expression, OPN is either not required for mammary primary tumor formation and growth in this system, or can be replaced by molecules other than BSP and vitronectin in mice that totally lack osteopontin.
Animals↗
[Clinical analysis of 10 cases of true histiocytic lymphoma].
OBJECTIVE: To explore the clinical and pathological characteristics of true histiocytic lymphoma. METHODS: The clinical and pathological data of 10 true histiocytic lymphoma patients admitted between 1986 and 1996 to our hospital was retrospectively reviewed. RESULTS: True histiocytic lymphoma accounted for 0.6% of non-Hodgkin's lymphoma(NHL) admitted in this period to our hospital. Enzymes associated with true histiocytic lymphoma were detected in all the 10 cases. The tumor cells were excluded from T/B lymphoid origin in 6 cases by immunohistochemistry. Eight cases received chemotherapy + irradiation +/- excision of primary lesion, 1 simply received irradiation and 1 received bone marrow transplantation. The response rate was 100%, with a 1, 3 and 5 year survival rate of 100%, 90% and 70%, respectively. The expected 10 year disease free survival rate is 40%. CONCLUSION: True histiocytic lymphoma is a rare subset of NHL. Those originated from lymph node are sensitive to chemotherapy and irradiation with a favorable prognosis.
Adolescent↗
[Nasea injection in the prophylaxis of gastrointestinal reaction induced by chemotherapeutic agents].
OBJECTIVE: To evaluate the efficacy and side effects of nasea injection for the prophylaxis of gastrointestinal reactions induced by chemotherapeutic agents, and compare them with those of kytril. METHODS: A multicenter, open, randomized self-crossover control trial was carried out. All the eligible patients were randomized into AB or BA group. The patients in AB group were treated by nasea in the first cycle and kytril in the second cycle, those in BA group were treated with the drugs in the reverse order. RESULTS: In patients enrolled in this study, 98 patients were evaluable for response, including 61 patients in DDP-arm and 37 in ADM-arm. Nasea was as effective as kytril in the control of anorexia, nausea and vomiting in the 0-6 hour period after chemotherapy. Nasea was significantly superior to kytril in the complete control of anorexia (38.8% vs 25.5%, P < 0.05) in the 0-24 hour period. The overall control rate of nausea by nasea in the 0-12, 0-18, and 0-24 hour period was 73.6%, 71.4%, 67.3%, respectively, which was significantly higher than that by kytril (65.3%, 61.2%, 48%). Nevertheless, in the control of vomiting in the 0-24 hour period, no statistical difference between the two drugs was observed. Nasea and kytril were equally effective in the control of anorexia, nausea and vomiting induced by DDP and ADM. The side effects of nasea were head heaviness, headache, dry mouth and constipation, etc. All of these were mild and comparable with kytril in their frequencies. CONCLUSION: Nasea can effectively prevent the gastrointestinal reaction induced by chemotherapeutic agents. The side effects of nasea are similar to those of kytril but its effect to alleviate gastrointestinal reactions is of longer duration.
Adolescent↗
[Treatment of advanced Hodgkin's disease: an analysis of 128 cases].
OBJECTIVE: To explore the rational treatment for advanced Hodgkin's disease. METHODS: A total of 128 patients with advanced Hodgkin's disease was included in this study. They could be divided into 3 groups according to the treatment they received. Patients in group 1 (n = 99) were treated by combination chemotherapy plus radiotherapy. Patients in group 2 (n = 24) were treated by chemotherapy alone. The remaining 5 patients in group 3 were treated by radiotherapy alone. The chemotherapeutic regimens used were MOPP, MOPP alternating with CHOP, or with ABVD. RESULTS: The overall response rate of 127 evaluable patients was 96.1%. The overall 1-, 3-, 5, and 10-year survival rate was 91.4%, 70.3%, 56.8% and 52.4%, respectively. The complete response (CR) rate in the 3 groups of patients was 69.7%, 58.3% and 100%, respectively. Patients in clinical stage III with no bulky mass, no systemic symptoms, and their tumor was predominantly of lymphocytic or nodular sclerotic type had better long term survival than those in clinical stage IV with bulky mass, systemic symptoms, and with lymphocyte depleting, mixed cellular tumor. Better long term survival was seen in patients who showed complete response to combined chemotherapy and radiotherapy. At least 6 cycles of chemotherapy were needed. CONCLUSION: Combination chemotherapy plus radiotherapy is effective in the treatment of advanced Hodgkin's disease.
Adolescent↗
[Simultaneous determination of fluoroacetamide and tetramethylendisulfotetramin with gas chromatography-mass spectrography].
Simultaneous determination of fluoroacetamide and tetramethylendisulfotetramin with gas chromatography-mass spectrography was established. The condition of separation, ways of extraction, the qualitative and quantitative determination of two compounds were discussed. The linear ranges of fluoroacetamide and tetramethylendisulfotetramin were 5-40 micrograms/ml and 0.5-20.0 micrograms/ml, respectively. The recoveries of these two compounds in different sample were 52.25%-94.30% and 61.85%-92.50%, respectively. The method was fast, sensitive and accurate.
Bridged-Ring Compounds↗
The clinical and epidemiological significance of serum PreS1/anti-PreS1 in HBV infection.
OBJECTIVE: To investigate whether PreS1 antigen and/or anti-PreS1 antibody in serum might have some significance different from routine hepatitis B virus (HBV) markers in hepatitis B virus infection. METHODS: Forty-six acute hepatitis B patients and 377 chronic persistent hepatitis patients were examined to assess the clinical significance of serum PreS1/anti-PreS1. HBV family clustering analysis was carried out in a village in Shanghai suburb area, and case-control study on role of PreS1 in HBV spreading was undertaken in Tangshan, Hebei province. RESULTS: PreS1 in HBsAg positive sera from chronic persistent hepatitis patients correlated with HBV DNA (P < 0.05), while the presence of PreS2 in HBsAg positive sera was much more common than that of PreS1 and had no correlation with HBeAg or HBV DNA. Among 46 patients with acute hepatitis B, the appearance of anti-PreS1 antibody in serum correlated with the disappearing or decreasing of both PreS1 and ALT. In antiviral treatment, PreS1 antigen turned negative much earlier than PreS2 and HBsAg in acute hepatitis B patients' sera. Family clustering analysis suggested that people with PreS1 positive in serum were more infective than those with HBsAg positive alone (P < 0.01). The case-control study showed that chronic hepatitis B patients with PreS1 positive in serum played a much more important role in HBV spreading than those with PreS1 negative (P < 0.01) and asymptomatic HBsAg carriers (P < 0.01), and the odds ratios (OR) were 3.8 and 3.2, respectively. CONCLUSIONS: Serum PreS1 closely correlates with active virus replication, and PreS1/anti-PreS1 status indicates the outcome of acute hepatitis B. The serum PreS1/anti-PreS1 marker is of some significance in HBV epidemiology as well.
Biomarkers↗
[Adrenal metastasis in primary lung cancer].
OBJECTIVE: To study the incidence, histologic types, clinical symptoms, response rates, and median survival time of adrenal metastasis in primary lung cancer. METHODS: The clinical data were retrospectively evaluated in 96 out of 6,976 patients with adrenal metastasis in primary lung cancer between January 1984 and January 1997. RESULTS: The incidence of adrenal metastasis was 1.38% (96/6,976) in 6,976 patients, 2.25% (37/1,643) in small cell lung cancer, 1.11% (59/5333) in non-small cell lung cancer. Abdominal pain and lumbago (excluding other reasons, for example, abdominal vertebrae and other abdominal organs were involved) occurred in 39% (37/96) in 96 patients. A overall response rate (CR + PR) was 43% in 54 patients who received chemotherapy (5 patients combined with irradiation) and could be evaluated. A median survival time was 7.17 (1-20) months in 96 patients. CONCLUSIONS: Primary lung cancer is easy to spread to adrenals. The incidence of adrenal metastasis is related to histologic types of primary lung cancer. Clinical symptoms include abdominal pain and lumbago. Surgical resection may be applied for a solitary adrenal metastasis after the primary tumor is removed. Chemotherapy is effective for patients with adrenal metastasis synchronous with other sites metastasis. The palliative radiation therapy can produce a high response rate in pain relief.
Adrenal Gland Neoplasms↗
[Expression of multidrug resistance-associated protein in human non-small cell lung cancer].
OBJECTIVE: To investigate the expression of the multidrug resistance-associated protein(MRP) gene and MRP in human non-small-cell lung cancer tissues and to determine whether such expression was related to cell type, differentiation, tumour size, lymph node metastasis and prognosis. METHODS: 92 paraffin-embeded lung tumor samples (43 squamous cell carcinoma, 49 adenocarcinoma) and 16 fresh non-small-cell lung cancer samples were examined by using immunohistochemistry method and the reverse transcription-polymerase chain reaction (RT-PCR) technology respectively. RESULTS: The expression of MRP mRNA and MRP were detectable in 31% (5-16) and 54% (50/92) of non-small-cell lung cancer specimens respectively. Eighteen(42%) squamous cell carcinoma specimens and thirty-two(65%) adenocarcinoma specimens showed positive immunostaining for MRP(P < 0.05). The expression of MRP was not related to tumour size, lymph node metastasis and cell differentiation significantly(P > 0.05). The five-year survival rate after operation of patients with MRP-positive tumours and MRP-negative tumours were 16% (8/50), 52% (22/42) respectively. Patients with MRP-positive tumours had shorter survival time than the MRP-negative patients significantly(P < 0.01). CONCLUSIONS: Adenocarcinoma had higher MRP expression than squamous carcinoma significantly, positive MRP immunostaining appears to be an independent indicator of poor prognosis in NSCLC.
Adult↗
[Clinical study of anastrozole in the treatment of postmenopausal women with advanced breast cancer].
OBJECTIVE: To study the effect and adverse reactions of anastrozole in the treatment of postmenopausal women with advanced breast cancer. METHODS: A multicenter, open, non-randomized and crossover clinical trial on the effect of anastrozole was conducted. Sixty-one postmenopausal women with advanced breast cancer were treated by oral anastrozole, 1 mg once every day. Each patient received 4-6 weeks of treatment. Plasma estradiol concentrations were determined 2 weeks before treatment and within 3 days after treatment discontinued. Effect and adverse reaction were evaluated. RESULTS: The response rate in 60 evaluable cases treated with anastrozole was 21.7% (CR = 0, PR = 13, MR = 2). The effect was correlated with postmenopausal time, previous endocrine therapy and ER status. Plasma level of estradiol was suppressed by 58.3%. The main adverse reactions, including nausea, fatigue, facial flush, were generally tolerable. CONCLUSION: As an aromatase inhibitor capable of decreasing plasma estradiol level, anastrozole is therapeutically effective for advanced breast cancer in postmenopausal women. Side effects are mild and tolerable.
Adult↗
[Expression of multidrug resistance-associated protein (MRP) and lung resistance protein (LRP) in human rectal carcinomas and its clinical significance].
OBJECTIVE: To investigate the expression and clinical significance of multidrug resistance-associated protein(MRP) and lung resistance protein(LRP) in rectal carcinoma. METHODS: Fiftyseven paraffin embeded primary rectal carcinoma specimens were examined by immunohistochemical staining. RESULTS: Of the 57 tumour specimens examined, 28(49.1%) and 24(42.1%) were positively immunostaned for MRP and LRP, respectively. Co-expression of the two proteins was observed in 10(17.5%) specimens. Positive immunostaning of either of the two proteins was not correlated with cell differentiation and Dukes stage (P > 0.05). Patients with MRP-positive tumours had shorter survival than MRP-negative patients (P < 0.05). No such correlation was found for LRP expression, nor was found between MRP and LRP expression. CONCLUSION: Positive MRP expression in rectal carcinoma appears to be an independant factor of prognostic significance. To examine its expression may help guide rational comprehensive therapy of rectal carcinoma.
Adult↗