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Biomedical subjects

F Filadoro

Publications and source records attributed to F Filadoro.

At least 37 records · Page 2Linked to original sources
PubMed1991

Effect of cyclosporin A on B cell maturation and differentiation.

The activation and differentiation of resting B cells into Ig secreting cells are regulated by T cells, macrophages and their secreted factors. The present study evaluated the effect of cyclosporin A (CsA) on this process. Peripheral blood lymphomonocytes (PBMC) drawn from healthy donors were stimulated with protein A (PA) or with lipopolysaccharides plus pokeweed (LPS+PWM) in either the presence or the absence of CsA. Phenotypic B cell changes and immunoglobulin production was then analyzed. The data revealed that CsA decreased the expression of B cell surface receptors of the activation phase, and enhanced the resting phase receptors. Different effects of CsA were found on B cell differentiation, depending on its induction by PA or LPS+PWM. In the first system, CsA decreased the expression of differentiation phase receptors and the secretion of free Ig. In cultures stimulated with LPS+PWM, CsA increased the differentiated phase receptors and Ig secretion. Thus, CsA seemed to act as a blocking agent of the activation phase and as a modulator of the differentiation phase and of IgG secretion, depending upon the antigen used for stimulation.

Antigens, Surface↗
PubMed1989

Role of alkaline protease and elastase in the adherence of Pseudomonas aeruginosa to WEHI cells.

Recent clinical isolates were tested for production of some extracellular factors such as alkaline protease and elastase. They were also assayed for adhesiveness to WEHI cells. It is well known that extracellular production of substances other than toxins is related to virulence and may increase adherence. The present investigation aimed to evaluate the role of extracellular proteins in adherence. Alkaline protease production was assayed using a test performed with casein as substrate while elastase activity was investigated with the elastin-congored method. Our results demonstrated that P. aeruginosa strains which are good alkaline protease and elastase producers adher better than those showing no or low protease and elastase activity.

Bacterial Adhesion↗
PubMed1985

[Sero-epidemiological results in subjects receiving a renal transplant].

In this study a sero-epidemiological investigation on 127 renal allograft recipients was examined. In these patients, treated with Cyclosporine A or conventional drugs, antibody response to various antigens (Cytomegalovirus, Herpes simplex, Varicellae/Zoster and Mycoplasma pneumoniae) was examined. The data were compared to the healthy population and dialyzed subjects.

Adolescent↗
PubMed1984

[Pulmonary infectious complications caused by cytomegalovirus in clinical kidney transplantation].

Infections constitute one of the main complications in organ allografts because of the immunosuppression status of the patient due to the own disease and the reduction of the immunoresponse dealing with the immunosuppressive therapy. Moreover the bacterial, micotic and parasitic infections show a lower incidence while viral infections affect more frequently the patients. On these basis the AA, report their experience on 162 kidney allografts with particular reference to cytomegalovirus pulmonary infections observed in nine patients. Five patients died of acute respiratory insufficiency while in three cases a complete resolution of the clinical picture has been observed. Finally one case underwent the association of pulmonary infection and irreversible graft failure due to rejection. The AA. conclude that the cytomegalovirus pulmonary infection is particularly riskfull in the non immunocompetent patients and stress the potent effect of the high titer specific immunoglobulins to prevent such often lethal complication.

Adult↗
PubMed1984

In vitro susceptibility tests with cefoperazone compared with cefotaxime, cefoxitin, cefuroxime, cephaloridine and gentamicin.

Minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs) and sensitivity disc tests of cefoperazone, cefotaxime, cefoxitin, cefuroxime, cephaloridine and gentamicin were determined from 300 Gram-positive and -negative isolates from kidney transplant recipients. Cefoperazone inhibited 50% of Pseudomonas aeruginosa strains at a concentration of 6.25 micrograms/ml. Only gentamicin had similar activity but if we consider the sources of our strains and the nephrotoxicity of the compound, its use is not suitable. Moreover, cefoperazone was able to inhibit from 50% to 90% of the other strains at variable concentrations between 0.39 micrograms/ml and 3.12 micrograms/ml.

Bacteria↗