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Biomedical subjects

F Frankel

Publications and source records attributed to F Frankel.

At least 19 recordsLinked to original sources
PubMed1999

Biological surface engineering: a simple system for cell pattern formation.

Biological surface engineering using synthetic biological materials has a great potential for advances in our understanding of complex biological phenomena. We developed a simple system to engineer biologically relevant surfaces using a combination of self-assembling oligopeptide monolayers and microcontact printing (muCP). We designed and synthesized two oligopeptides containing a cell adhesion motif (RADS)n (n = 2 and 3) at the N-terminus, followed by an oligo(alanine) linker and a cysteine residue at the C-terminus. The thiol group of cysteine allows the oligopeptides to attach covalently onto a gold-coated surface to form monolayers. We then microfabricated a variety of surface patterns using the cell adhesion peptides in combination with hexa-ethylene glycol thiolate which resist non-specific adsorption of proteins and cells. The resulting patterns consist of areas either supporting or inhibiting cell adhesion, thus they are capable of aligning cells in a well-defined manner, leading to specific cell array and pattern formations.

3T3 Cells↗
PubMed1999

Do stimulants improve self-esteem in children with ADHD and peer problems?

The self-esteem of children with Attention Deficit Hyperactivity Disorder (ADHD) has been shown to be low. The effects of stimulant medication upon their self-esteem have not been systematically studied. The present study employed a reliable self-report instrument to measure the self-esteem of children with ADHD medicated with stimulants vs. those who were unmedicated. Results showed that stimulants were associated with significantly higher self-esteem. Children with ADHD prescribed stimulants reported feeling more intelligent and more popular than unmedicated children with ADHD. Children with ADHD and Oppositional Defiant Disorder (ODD) prescribed stimulants reported feeling better behaved. Significant correlations indicated that higher doses were associated with higher self-esteem. The present results suggest a need for a well-controlled study to determine if stimulants were responsible for the observed differences in self-esteem.

Analysis of Variance↗
PubMed1998

Videotaped assessment of parenting practices: a preliminary report.

Assessment of parenting skills was based upon a standardized videotape of vignettes of parents reading a magazine while their children were playing, playing with their children, or trying to get them to clean up. Parents who viewed this videotape came from 40, 2-parent families referred to an outpatient clinic for behavior problems (Behavior Problem group) or 20 nonreferred 2-parent families (Nonclinic group). They were compared on the degree to which they selected: (1) positive responses to children's prosocial behavior (praise), (2) ignoring children's inappropriate behavior (ignore), and (3) punishing children's aversive behavior (punish). The Nonclinic group was more accurate than the Behavior Problem Group and mothers were more accurate than fathers in their selection of praise to children's prosocial behavior. Nonclinic mothers were more accurate than all other combinations of Group and Parent Sex in their selection of ignore for children's inappropriate behavior. Sensitivity calculations suggested that this instrument could potentially be useful as a screening tool in clinical pediatric practice and early intervention. Implications for nursing research and practice were discussed.

Adult↗
PubMed1997

Use of the Child Behavior Checklist and DSM-III-R diagnosis in predicting outcome of children's social skills training.

A standardized 12-week cognitive-behavioral social skills package in which parents assisted children with socialization homework assignments, was presented to 52 nonpsychotic outpatient boys, many of whom were diagnosed with DSM-III-R Attention Deficit-Hyperactivity Disorder and Oppositional Defiant Disorder. Results demonstrated that the Thought Problems factor of the mother-completed Child Behavior Checklist aided in predicting changes in teacher-rated aggression. DSM-III-R diagnosis of Oppositional Defiant Disorder predicted changes in teacher-rated withdrawal subsequent to treatment. The need for research to determine which children benefit from which treatment was discussed.

Aggression↗
PubMed1997

Parent-assisted transfer of children's social skills training: effects on children with and without attention-deficit hyperactivity disorder.

OBJECTIVE: Previous research has demonstrated that peer rejection is a significant part of the clinical presentation of many children with attention-deficit hyperactivity disorder (ADHD). Outcome studies of treatment interventions have typically failed to show generalization of treatment gains to the home and classroom. This has been especially true for children who have comorbid oppositional defiant disorder (ODD). The present study was intended to demonstrate generalization of an outpatient social skills training program when parents were trained in skills relevant to their child's social adjustment. METHOD: Thirty-five children with ADHD and 14 children without ADHD were given 12 sessions of treatment (treatment group). Outcome was compared with 12 children with ADHD and 12 children without ADHD who were on a waitlist for treatment (waitlist group). Nineteen children with ODD were in the treatment group and five in the waitlist. Stimulant medication was prescribed for all children with ADHD. RESULTS: Subjects with ADHD showed improvement comparable with that of subjects without ADHD on all teacher- and parent-reported measures of peer adjustment and social skills, except teacher-reported withdrawal. Children with ODD had outcome comparable with that of children without ODD. Effect sized ranged from 0.93 to 1.34 indicating that the average treatment group subject was better off than 83.4% of waitlist subjects on outcome measures. CONCLUSIONS: The present results suggest that children with ADHD are best heiped by a combination of social skills training for themselves, collateral training for their parents and stimulant medication.

Analysis of Variance↗
PubMed1993

Using patient reports to assess health-related quality of life after total hip replacement.

Data on disease severity, co-morbidity, and process of care were obtained from the medical records of 356 patients without rheumatoid arthritis undergoing a first unilateral total hip replacement at four teaching hospitals in California and Massachusetts. Socio-demographic characteristics, functional status prior and subsequent to hospitalization, and improvement in health status were measured with a patient questionnaire 12 months after discharge. Completed questionnaires were received from 284 patients, a response rate of 79.8%. The questionnaire was acceptable to patients, reliable, and had good construct validity. The data indicate substantial benefits from hip arthroplasty. As expected, pre-surgical functioning was a strong predictor of outcomes 1 year after surgery. Controlling for pre-surgical functioning, age was not related to outcomes.

Activities of Daily Living↗
PubMed1993

A brief test of parental behavioral skills.

The present study details the development of a brief, criterion-referenced, multiple-choice test designed to assess parental behavioral skills. A nine item parent-child conflict questionnaire was developed and administered to parents of children referred to two clinic locations and to parents of nonreferred children. Factor-analytic techniques were used to refine the scale. Parents of nonclinic children obtained higher scores than parents of clinic-referred children. Further, only parents of nonclinic children scored better as the number of children reared increased. The effect of education level was not significant. Implications of these results are discussed.

Adult↗
PubMed1993

Sources of Family Annoyance (SOFA): development, reliability, and validity.

The Sources of Family Annoyance (SOFA) is a brief questionnaire intended to measure chronic stressors experienced by fathers and mothers in the home. Two scales, Household Negatives and Child Negatives, were derived from factor analysis. Experiment 1 demonstrated that the instrument discriminated a group of parents of psychiatric clinic-referred children from nonreferred parents. Experiment 2 demonstrated that the instrument discriminated mothers who were receiving public assistance from mothers in a community sample. Results suggest that the SOFA is a reliable and valid brief parent stress assessment instrument that may be useful in family-based interventions to reduce stress and improve the course of pediatric illness.

Adult↗
PubMed1992

Cluster analysis of child behavior checklists of 6 to 11-year-old males with varying degrees of behavior disorders.

Achenbach Child Behavior Checklist ratings were collected on boys aged 6 to 11 years screened as not having significant behavior problems, as clinic-referred boys, and as boys diagnosed as having Attention Deficit-Hyperactivity Disorder alone or together with Oppositional-Defiant Disorder or Conduct Disorder. Cluster analysis resulted in a classification system related to DSM-III-R classification of the boys and parent self-report of mildly antisocial acts. Clusters also enhanced the ability of the instrument to detect Attention Deficit Hyperactivity Disorder and disruptive behavior disorders.

Attention Deficit Disorder with Hyperactivity↗
PubMed1990

Platelet alpha 2-adrenergic receptor binding to 3H-yohimbine and personality variations in normals.

The authors examined platelet alpha 2-adrenergic receptor binding to 3H-yohimbine and several personality variables in 58 adult males in a campus community. Subjects with high receptor Bmax levels exhibited personality traits of less social inhibition and more sensation seeking and thrill seeking behavior, were more playful and autonomous, and came from healthier, more intact families. The results also suggested that high affinity states of platelet alpha 2-adrenergic receptors (low Kd) correlate with traits suggestive of stability, i.e., Autonomy, Dominance, Nurturance, Order, Succorance, and General Sensation Seeking Scale of Zuckerman, while low affinity states (high Kd) of platelet alpha 2-receptors correlate with psychopathological traits of Dependence, Exhibitionism, and Paranoia.

Adult↗
PubMed1989

Platelet MAO activity and personality variations in normals.

The authors examined platelet MAO activity and several personality variables in 58 non-clinic-referred healthy adult males. The psychological tests administered consisted of the Minnesota Multiphasic Personality Inventory, the Personality Research Form-E, and the Zuckerman Sensation Seeking Scale. The findings replicated previous findings in the literature.

Adult↗
PubMed1988

Cloning of estrogen-regulated messenger ribonucleic acids from rat uterus.

A cDNA library prepared from the mRNA of uteri of estrogen-stimulated immature female rats was constructed in lambda gt10. Differential screening of the hybrid phages was performed using control and stimulated cDNAs as probes. Selected clones were then characterized by Northern and Southern blot hybridizations. In all, eight unique clones corresponding to estrogen-stimulated messages in rat uterus were identified. These clones hybridized to uterine mRNAs varying in size from 1.4-8.4 kilobases. Three of the clones were characterized as coding for three different types of collagen, and one as coding for smooth muscle actin. These are described in more detail elsewhere. The kinetics of increase in estrogen-regulated messages was examined. After a single injection of estradiol, five clones, including the three collagen mRNAs, showed two peaks of accumulation, at 4 and 24 h. Messages of two other clones were maximal at 12 h. The actin clone hybridized to mRNAs with peaks at 4 h for cytoskeletal actins and 8-12 h for smooth muscle actins. Sequential 24-h injections of the hormone produced multiple peaks of mRNA accumulation with a timing consistent with the kinetics found after a single injection of hormone. The multiple injections, however, did not result in enhanced mRNA accumulation for any of these clones. In fact, several messages showed suppressed accumulation with continued estradiol administration. Accumulated inhibitory factors in uterine cells may be responsible for this refractory condition. Except for the actin mRNA, the estradiol-stimulated mRNAs were expressed mainly in uterus and ovary. These clones may be useful in studies on the mechanism of action of estrogenic hormones and their tissue-specific regulation of gene expression.

Animals↗
PubMed1987

Reward value of prosodic features of language for autistic, mentally retarded, and normal children.

The present study pursued hypotheses relevant to prosodic abnormalities in the expressive language of autistic children. An operant paradigm was employed to measure the intrinsic reinforcing value of a verbally presented story for autistic, mentally retarded, and normal groups controlling for chronological age and performance mental age. Results suggested that both autistic and mentally retarded children could perceive prosodic cues, although only mentally retarded children are able to use them in their expressive language. It was also suggested that linguistic stimuli may have less intrinsic reinforcement value for autistic and mentally retarded children than for normal children. Implications for future research and treatment were discussed.

Autistic Disorder↗
PubMed1985

Behavioral treatment approaches to pathological unsocialized physical aggression in young children.

This paper presents some hypotheses regarding the motivation of pathological unsocialized physical aggression in children and also reviews behavioral treatment. Tentative leads were offered as to extrinsic and intrinsic determinants. Among the most promising of these were parent-child interactive and attributive factors, and deficits in information processing in social situations. It was hypothesized that some of these factors might not be specific to pathological unsocialized physical aggression but are characteristic of children with behavior problems. Under this hypothesis, few studies were found which employed appropriate control groups. It was also hypothesized that the study of extrinsic and intrinsic factors for pathological unsocialized physical aggression may improve the design of treatment programs.

Adolescent↗
PubMed1984

Stimulus overselectivity in autistic and mentally retarded children--a research note.

Groups of autistic and mentally retarded children were compared for the degree of stimulus overselectivity manifested during test probes after discrimination learning. Results demonstrated that stimulus overselectivity was a function of diagnostic category when groups were equated for performance IQ and mental age. Procedures used to obtain IQ as well as discrimination learning rate were also shown to be equivalent. Pulse rate response suggested that the test probes may be assessing performance during the early trials of a transfer problem for both groups of children. Implications for 'one-look' models of discrimination learning were discussed.

Arousal↗
PubMed1983

Translocation of cytoplasmic estrogen receptors to the nucleus: immunohistochemical demonstration utilizing rabbit antibodies to estrogen receptors of mammary carcinomas.

Rabbit antibodies to cytoplasmic estrogen receptors (ER) of human breast carcinoma were utilized for investigating steroid-triggered in-vitro translocation of cytoplasmic ER to the nuclear compartment of the estrogen target cells. The immunofluorescent method (IF) previously described (S Raam et al., Eur J Cancer Clin Oncol 18: 1-12, 1982) was employed for immunohistochemical localization of ER. Four cases of normal endometrium, two cancers of the endometrium, and MCF-7 human breast cancer cells were maintained in a steroid free medium and exposed at 37 degrees C for two hours to growth medium alone (control) or to 2.5, 25 or 250 nanomoles of estradiol (E2), diethylstilbestrol (DES), or monohydroxytamoxifen (OH-TX). At the end of the incubation period the cells were processed for intracellular localization of ER. Complete traslocation of IF from the cytoplasm to the nuclear compartment was evident in all normal endometrial cells exposed to E2, DES or OH-TX for two hours. While cells from the endometrial cancer 'S', like the normal cells, translocated IF to the nucleus, cells of another cancer ('KLE') failed to translocate when exposed to E2 or OH-TX. Partial translocation was evident in 'KLE' cells exposed to DES. In MCF-7 cells grown in the absence of E2, IF was exclusively cytoplasmic. When these cells were exposed to the hormones, 50% showed a complete transfer of IF to the nucleus; in 40% a delayed response was evident; 10% failed to translocate. The results revealed the suitability of anti-ER antibodies for investigating the intracellular dynamics of ER in target cells responding to estrogens or antiestrogens.

Animals↗