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Biomedical subjects

F Frigeri

Publications and source records attributed to F Frigeri.

61 records · Page 4Linked to original sources
PubMed1979

A study of bovid herpesvirus 2 infection in calves inoculated intradermally or intranasally.

Bovid herpesvirus 2 infection was studied in calves exposed to the virus by intradermal inoculation of the skin of the left cheek or by nasal spray. In either case a localised infection developed and virus replication was shown to occur mostly in the tissues of its primary localisation, i.e. the skin of the left cheek or the nasal mucosa. There were neither secondary lesions, except at the site of virus injection, nor any serious systemic involvement on the part of the animals. The virus was also recovered from some areas of the skin (right cheek, perineum and scrotum) that were free of macroscopic lesions; moreover, intranuclear inclusions were found in several tissues of the nervous system (brain, superior cervical, stellate and Gasserian ganglia) which did not show any signs of inflammatory or degenerative changes. These findings suggest that the skin and the nervous system play an important role in the naturally-occurring disease since they could be the sites where the virus is maintained latently in the host.

Administration, Intranasal↗
PubMed1978

Distribution of Bovid herpesvirus 2 in calves inoculated intravenously.

The behavior of Bovid herpesvirus 2 in the skin, lymph nodes, and nervous system of calves injected intravenously with the virus was studied. After a latent phase that lasted up to 40 hours after inoculation, the virus produced a systemic infection which was characterized by primary localization of virus in the skin, lymph nodes, and nervous system. Between postinoculation days 7 and 12, the infection tended to persist in the skin only. The finding of intranuclear inclusions in the pyramidal cells of the cerebral cortex and in the neurons of the superior cervical and stellate ganglia supports the hypothesis that the virus might utilize the structures of the nervous system for its replicative cycle in the calf.

Animals↗
PubMedDate not supplied

A study on neonatal calf diarrhea induced by rotavirus.

This review summarizes the results of a study on rotaviruses isolated from calves affected by neonatal diarrhea. The results indicated that rotavirus infection is widespread and supported the evidence for an etiologic role of these viruses in neonatal diarrhea. Differences in virulence among bovine rotaviruses appeared also to be confirmed. Conventionally reared calves were fully susceptible to the experimental infection induced by rotaviruses originating from heterologous hosts, i.e. monkeys, pigs and rabbits. When rotavirus strains of bovine, simian and rabbit origin were compared by cross neutralization tests, it was found the simian and porcine strains were indistinguishable and both appeared to relate antigenically to the bovine strain. Finally, it was proven that feeding newborn calves with colostrum and first milk of their dams, previously vaccinated with an inactivated adjuvanted rotavirus vaccine, could prevent the neonatal diarrhea from occurring.

Animals↗
PubMedDate not supplied

Hb-M "Hyde Park": a de novo mutation, identified by mass spectrometry and DNA analysis.

BACKGROUND: Structural hemoglobinopathies usually are inherited as autosomic dominant traits; de novo mutations are uncommon. Analytical and preparative procedures for the characterization of an abnormal hemoglobin are complex and time-consuming. Mass spectrometer analysis allows a rapid identification of the amino acid substitution. METHODS AND RESULTS: A cyanotic 7-year-old girl was found to have 16% methemoglobin. Laboratory data showed the presence of an abnormal hemoglobin, which was isolated by collecting the abnormal peak from DEAE and globin chains from CM52. The amino acid substitution was rapidly identified by FAB mass spectroscopic analysis, leading to the recognition of HbM Hyde Park. These data were confirmed by molecular analysis (Southern blot and DNA sequencing). Neither the parents nor a sister showed any abnormality; non-paternity was excluded by blood group serology and HLA typing. CONCLUSIONS: This is a case of HbM Hyde-Park arising as a de novo mutation. FAB mass spectroscopic analysis is a rapid and useful analytical method for identifying aminoacid substitution.

Blotting, Southern↗
PubMedDate not supplied

Preliminary studies on the diffusion of bovid herpesvirus 2 in experimentally infected calves.

This preliminary study on the diffusion of the Bovid herpesvirus 2 (BHV2) in calves inoculated intravenously, allowed to identify the target organs of the virus. In particular, in the first phase of the infection, i.e. post infection day (PID) 2, the virus resulted extensively widespread in the body of the calf, whereas in a subsequent phase, PID6, the virus was recovered only from the skin, the lymph nodes and the nervous system. Finally virus was not recovered in any of the tissues obtained from the calf killed at PID 12. Based on the results it might be speculated that, under the experimental conditions of these tests, the infection of cattle by BHV2 develops through two stages. The first stage would be characterized by a systemic condition in that the virus is present in the whole organism; the second stage which follows, consists in a localized infection, the virus being present only in some districts of the organism (skin, lymph nodes, nervous system) which could be considered as the definitive target sites for virus replication.

Animals↗
PubMedDate not supplied

Effect of alpha IFN on unaggressive immunoproliferative disorders.

BACKGROUND: alpha-IFN is reported to be an effective treatment for a number of lymphoproliferative diseases. Little information is available at present on its effect in unaggressive immunoproliferative disorders. STUDY DESIGN AND RESULTS: In a prospective non randomized study, 57 patients with IgG or IgA MGUS, smouldering myeloma or stage I MM treated with alpha-IFN (3 MU 3 times a week for at least 6 months) were compared to 129 untreated similar patients. Four patients in the IFN group showed a monoclonal component reduction > 50% versus none in the control group, and 25% of patients suffered disease progression (MC increase > 50% and/or osteolytic lesions) in the IFN group as compared to 18% in the control group. CONCLUSIONS: alpha-IFN administration at the dose used is ineffective for the majority of patients with slowly proliferating immunoproliferative disorders; only a small subgroup of them may benefit from such a treatment.

Case-Control Studies↗