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F G Priest

Publications and source records attributed to F G Priest.

38 records · Page 3Linked to original sources

Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus.

Comparative 16S rRNA sequence analysis has demonstrated that the genus Bacillus consists of at least five phyletic lines. rRNA group 3 bacilli of Ash, Farrow, Wallbanks and Collins (1991) comprising Bacillus polymyxa and close relatives is phylogenetically so removed from Bacillus subtilis, the type species of the genus and other aerobic, endospore-forming bacilli that they warrant reclassification in a new genus Paenibacillus. The genus Paenibacillus can be readily distinguished from other Bacillus groups using a battery of phenotypic characters and a highly specific gene probe based on 16S rRNA.

Bacillus↗

A polyphasic taxonomic study of thermophilic bacilli from a wide geographical area.

Two hundred and thirty-four thermophilic Bacillus strains isolated from geographically widespread locations were examined by phenotypic characterisation followed by numerical analysis. The strains were distributed between eighteen cluster-groups which were subsequently evaluated in DNA base composition and DNA sequence homology studies. The inclusion of type and reference strains unambiguously identified strains related to B. licheniformis, B. pallidus, B. smithii, B. stearothermophilus, B. thermocloacae and B. thermoglucosidasius. Other reference strains included in distinctive groups were 'B. caldotenax', together with 'B. caldovelox' and 'B. caldolyticus', B. kaustophilus and 'B. thermodenitrificans'. An emended description of B. kaustophilus is provided. It is proposed that 'B. caldotenax' and 'B. thermodenitrificans' should be accepted as validly described species. Members of other clusters that appeared to have distinctive characteristics, including beta-glucanase production and the ability to degrade tyrosine, may provide the nuclei of further novel species.

Bacillus↗