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Biomedical subjects

F G Serio

Publications and source records attributed to F G Serio.

At least 19 recordsLinked to original sources
PubMed2005

Interleukin-18 concentrations and the pathogenesis of periodontal disease.

BACKGROUND: Interleukin-18 (IL-18) is reported as an important regulatory cytokine in non-oral inflammation. Our objective was to compare the concentrations of IL-18 within diseased and healthy human gingiva with concentrations of other T(H)1 and T(H)2 cytokines to determine possible effects of IL-18 on gingival inflammation. METHODS: Gingival biopsies were obtained prior to routine tooth extraction. Gingiva was grouped by the depth of the adjacent gingival sulcus: < or =3 healthy (featuring no bleeding on probing) and > or =3 mm diseased (featuring bleeding on probing). Diseased gingiva was subdivided into 3, 4 to 6 and >6 mm groups. Gingival interleukin (IL)-2, IL-4, IL-6, IL-10, IL-12, IL-18, and interferon (IFN)-gamma concentrations were assessed by enzyme linked immunosorbent assay (ELISA). Data were compared by factorial analysis of variance and the Pearson's correlation test. RESULTS: Concentrations of IL-2, IL-4, IL-6, IL-10, IL-18, and IFN-gamma adjacent to 4 to 6 mm diseased sites were greater than adjacent to < or =3 mm healthy sites (P <0.001). IL-12 concentrations were lower within diseased than within healthy gingiva (P <0.001). IL-6 and IL-18 concentrations were greater adjacent to >6 mm sites compared to healthy sites (P <0.001); the concentrations of the other cytokines (except IL-12) were similar to healthy sites. IL-6 and IL-18 concentrations were positively correlated, and IFN-gamma and IL-12 negatively correlated, with the adjacent gingival sulcular depth. CONCLUSIONS: Periodontal inflammation may not successfully resolve because of accumulation of IL-6 and IL-18, and decreased concentrations of IL-12, within diseased gingiva. Because of the highly significant correlation between IL-18 concentration and gingival sulcular depth, IL-18 may be a useful target for either preventive or palliative therapy for periodontitis.

Adolescent↗
PubMed2004

Interleukin-11 and IL-17 and the pathogenesis of periodontal disease.

BACKGROUND: Interleukin (IL)-11 and IL-17 are cytokines that modulate the inflammatory process and have not been assessed within normal or inflamed gingival tissues. Our purpose was to compare concentrations of human IL-11 and IL-17 within healthy and diseased human gingiva to determine their possible role in the initiation or progression of periodontal diseases. METHODS: Biopsies from healthy (non-hemorrhagic gingiva adjacent to a < or = 3 mm gingival sulcus) and diseased gingiva (hemorrhagic gingiva adjacent to a > or = 3 mm periodontal pocket) were studied. IL-11, IL-17, RANTES, and IL-6 concentrations were assessed within solubilized gingival biopsies by enzyme-linked immunosorbent assay. Data were compared by factorial analysis of variance and a post-hoc Tukey honestly significant difference (HSD) test. Regression analysis and partial correlation analysis (adjusted for sample weight) were also used to determine correlations between the variables. RESULTS: Interleukin-11 concentrations were highest within gingiva adjacent to 3 mm diseased pockets (P < 0.001), and IL-17 concentrations were highest at 4 to 5 mm sites compared to other sites (P < 0.001). Gingival concentrations of both cytokines were significantly lower in gingiva adjacent to a > or = 6 mm pocket. RANTES concentrations were significantly greater in gingiva adjacent to > or = 6 mm pockets than in tissues derived from other sites (P < 0.001). IL-11, IL-6, and RANTES concentrations were significantly correlated with sulcular depth. CONCLUSIONS: Gingival concentrations of IL-11 and IL-17 are different in diseased gingiva adjacent to 3, 4 to 5, and > or = 6 mm pockets, suggesting that their concentrations change as a consequence of the progression of gingivitis to periodontitis and that both cytokines could have a significant role in this progression. These data may be useful for the design of procedures for prevention of periodontal disease.

Adolescent↗
PubMed2001

Leptin within healthy and diseased human gingiva.

BACKGROUND: Plasma leptin concentrations are reported to be elevated in patients with inflammatory diseases. There is no consensus concerning the biological mechanism for this phenomenon. To date, tissue leptin concentrations have not been assessed within normal or inflamed gingiva. The purpose of this study was to assess concentrations of human leptin within healthy and diseased gingiva to define its possible role in periodontal disease progression. METHODS: Healthy (non-hemorrhagic gingiva adjacent to a < or =3 mm gingival sulcus) and inflamed gingiva (hemorrhagic gingiva adjacent to a > or =3 mm periodontal pocket) were studied. Leptin, vascular endothelial growth factor (VEGF; to assess potential vascular expansion), and interleukin-6 (IL-6; to assess periodontal disease activity and severity) concentrations were assessed within solubilized gingival biopsies by enzyme-linked immunosorbent assay. Data were grouped by sulcular depth and compared by factorial analysis of variance, regression analysis, and Scheffé comparisons. RESULTS: Leptin concentrations were highest within gingiva adjacent to a < or =3 mm sulcus and progressively declined within gingiva adjacent to a > or =3 mm sulcus. VEGF concentrations were highest within gingiva adjacent to 4 to 6 mm pockets and nearly equivalent in healthy (< or =3 mm sulcus) and severely diseased gingiva (>6 mm sulcus). IL-6 was positively correlated and leptin negatively correlated with adjacent probing depth; IL-6 concentration was significantly higher and leptin significantly lower in gingiva adjacent to >6 mm pockets compared to sites adjacent to <6 mm pockets (P<0.001). CONCLUSIONS: Human leptin is present within healthy and marginally inflamed gingiva and decreases in concentration as the adjacent probing depth increases. When leptin concentrations decreased (> or =3 mm sulcus), VEGF concentrations increased, suggesting that leptin could be released from gingiva coincident to vascular expansion. Thus, gingiva, in addition to adipose tissue, could be a source of circulating leptin in patients with periodontal disease. This possibility requires further investigation.

Adolescent↗
PubMed1999

Vascular endothelial growth factors and progression of periodontal diseases.

BACKGROUND: Tissues become hemorrhagic and edematous coincident to periodontal diseases; however, there is little information concerning the biologic mechanisms which may produce these changes. Vascular endothelial growth factor (VEGF) is a macromolecule which enhances blood vessel growth and permeability. However, there is no information concerning gingival VEGF concentrations within normal or diseased gingiva. The purpose of this study was to assess changes in gingival concentrations of VEGF during initiation and progression of periodontal diseases and compare them to changes in the number of blood vessel profiles and concentration of recognized markers of periodontal disease severity (interleukin-6[IL-6]). METHODS: Normal (non-hemorrhagic gingiva adjacent to a < or =3 mm gingival sulcus) and inflamed gingiva (hemorrhagic gingiva adjacent to a < or =3 mm, 4 to 6 mm, or >6 mm periodontal pocket) were studied. VEGF and IL-6 concentrations were assessed by ELISA and the number of blood vessels determined by histomorphometric techniques. Data were placed into one of the following groups: < or =3 mm, normal; < or =3 mm, diseased; 4 to 6 mm, diseased; and >6 mm, diseased. These groups were compared by factorial ANOVA and Scheffe comparisons. In addition, groups were compared by simple and multiple regression and regression ANOVA to determine possible correlations between them. RESULTS: VEGF and IL-6 concentrations were significantly lower within normal than within diseased gingiva. The number of blood vessel profiles and mean IL-6 concentrations were highest in diseased tissues adjacent to >6 mm sulci and were significantly correlated with sulcular depth (P <0.001). In contrast, VEGF concentrations were highest within diseased gingiva adjacent to 4 to 6 mm periodontal pockets (P <0.001) and were not correlated with sulcular depth. CONCLUSIONS: VEGF may be a factor in initiation and progression of gingivitis to periodontitis, possibly by promoting expansion of the vascular network coincident to progression of the inflammation.

Analysis of Variance↗
PubMed1999

Mucogingival surgical procedures: a review of the literature.

This article provides an in-depth review of the literature on mucogingival surgical techniques. Indications and contraindications of various surgical procedures are discussed with reference to the literature. Surgical techniques and indications for increasing the zone of keratinized tissue, such as free autogenous grafts, applications of freeze-dried skin, and dermal matrix allografts, are described. Procedures to attain root coverage, such as various autogenous grafts and guided tissue regeneration techniques, along with application of chemicals such as citric acid to improve their success, are also described.

Acid Etching, Dental↗
PubMed1999

Clinical rationale for tooth stabilization and splinting.

Tooth mobility can be a consequence of periodontal inflammation, attachment loss, and occlusal trauma. At times, this mobility may be tolerable, whereas at other times mobility may contribute to occlusal instability and/or patient discomfort. This article describes the clinical rationale for and the effects of splinting mobile teeth.

Bite Force↗
PubMed1999

Periodontal trauma and mobility. Diagnosis and treatment planning.

With the dearth of well-controlled human clinical studies, it is still impossible to answer the question of whether occlusal trauma modifies the progression of attachment loss resulting from inflammatory periodontal disease and the companion questions related to the treatment of occlusal trauma and mobility. Teeth with stable mobility are apparently at no greater risk of attachment loss than nonmobile teeth. Increasing mobility is a concern that must be addressed by inflammatory control, occlusal adjustment, and perhaps some type of stabilization or splinting of the tooth in question. In addition, greater attachment gains have been noted when occlusal adjustment was included as part of surgical therapy. There is no question that aspects of occlusal therapy have an empiric base. It is incumbent on the clinician to examine for, diagnose, and treat trauma from occlusion to stabilize the dentition. Proper occlusal management assists in maintaining the patient's natural dentition in a state of health and comfortable function.

Dental Occlusion, Traumatic↗
PubMed1998

Factors for progression of periodontal diseases.

Progression factors for periodontal diseases have been suggested by in vitro study of peripheral blood and gingival cells; however, those factors are not established in vivo. This investigation assessed biopsies of three groups of gingival tissues: those adjacent to a 1) < or =3 mm (normal), 2) 4-6 mm, and 3) >6 mm gingival sulcus, to determine changes in the gingival microenvironment coincident to the progression of periodontal disease. Superoxide dismutase (SOD) and catalase activity, and IL-12 and bcl-2 levels, were decreased at >6 mm; total protein and IL-6 concentrations were increased adjacent to >6 mm, as compared to < or =3 and 4-6 mm, sites. Apoptotic cells were evident only within gingiva adjacent to >6 mm sites. These data suggest that IL-12 is an important factor in the shift from a TH1 to TH2 cell profile and that a favorable gingival microenvironment for hyperinflammation may develop coincident to progression of periodontal diseases due to decreased bcl-2 and increased IL-6 concentrations within gingiva. These changes in the gingival microenvironment could impair apoptosis and promote enhanced release of reactive oxygen species (ROS) by phagocytes; decreased catalase and SOD activity could promote accumulation of ROS and result in additional tissue destruction.

Analysis of Variance↗
PubMed1998

A method for determining patient oral care skills: The University of Mississippi Oral Hygiene Index.

The plaque control record (O'Leary index) appears to be a commonly used oral hygiene index for assessing oral health skills. This index provides sufficient information for patient education; however, the time involved in data collection reduces its value. Most other indices limit the number of teeth and surfaces and function well for researchers, but are limited for patient education. A new oral hygiene index was developed based on the concepts of the Periodontal Screening and Recording (PSR). The highest score obtained in each buccal and lingual sextant is recorded. In addition, proximal and gingival plaque are noted separately. This study assesses the index for inter- and intrarater reliability and validity. Two calibrated hygienists examined 47 patients 3 times. The University of Mississippi Oral Hygiene Index (UM-OHI) was recorded for times 1 and 3, the O'Leary for time 2. There was a strong positive correlation between scores obtained for both hygienists for each method and repetition. The intrarater reliability was high for the 2 methods and also over time for the UM-OHI. Pearson's correlation coefficients ranged from 0.79 to 0.92. Paired t-tests used to compare scores for the 2 hygienists over time showed significant differences. Despite observer bias, these data seem to indicate that the UM-OHI has sufficient reliability and validity to be used as a health education teaching tool.

Calibration↗
PubMed1991

Plasma cell gingivitis of unusual origin. A case report.

Plasma cell gingivitis is a rare benign condition of the gingiva. It is marked by a dense infiltrate of normal plasma cells separated into aggregates by strands of collagen. It is a hypersensitivity reaction to some antigen, often flavorings or spices. The importance of this lesion is that it may cause severe gingival inflammation, discomfort, and bleeding and may mimic more serious conditions. Plaque control and conventional periodontal therapy alone will not cure this disease. The etiologic agent must be identified and the substance eliminated from use. This report outlines a case of plasma cell gingivitis which may have been brought on by the use of red peppers in cooking.

Adult↗
PubMed1991

Diagnosis and management of commonly occurring oral vesiculoerosive disorders.

Vesiculoerosive disorders are often difficult to diagnose and manage. The oral cavity is often overlooked as a source of diagnostic signs of dermatologic disease. In some disorders, oral manifestations precede cutaneous signs by as much as one year. The authors describe the oral presentation and management of lichen planus, allergic stomatitis, pemphigus vulgaris, and cicatricial pemphigoid, to enable the physician to recognize, diagnose, and treat commonly encountered oral manifestations of dermatologic disease.

Adult↗
PubMed1991

Periodontal diseases: a review.

Periodontal diseases are a collection of disorders that may affect patients throughout life. The most common form of periodontal disease, gingivitis, which affects only the soft tissues, is seen in children, adolescents, and adults. Periodontitis, which destroys the bone supporting a tooth, is found more commonly in adults over the age of thirty-five but may be present in a variety of forms in children after three years of age. These diseases are caused by bacterial plaque but may be modulated by systemic diseases, immunologic compromise, heredity, and other contributing factors. Periodontal pathoses may be an indication of an underlying systemic condition such as leukemia or human immunodeficiency virus infection. The standard treatment of periodontal diseases is the control of intraoral plaque. This may be accomplished using mechanical, chemotherapeutic, and surgical means.

Humans↗
PubMed1988

The effect of polishing pastes on composite resin surfaces. A SEM study.

CLINICALLY, COMPOSITE RESINS can be polished to a smooth surface at the time of placement using a variety of polishing methods. Subsequently maintaining the finish on recalls needs to be evaluated. The purpose of this investigation was to compare surface textures of a small particle composite resin (average particle size 5 microns) after polishing with prophylaxis and polishing pastes. Each specimen of the small particle composite resin, PrismaFil, was finished and polished to a smooth surface with Soflex discs. This was the control polish. The specimens were then polished with a rotating rubber prophylaxis cup with seven different prophylaxis and polishing pastes. All the samples were then prepared for scanning electron microscopy (SEM) evaluation and photographed at 750x to compare the effects of polishing on the surfaces of composite resins. SEM photographs were also made of a sample polished with Soflex discs (SF) and with a dry rubber cup (RC). The seven prophylaxis and polishing pastes were Command Ultrafine Lustre Paste (CULP), Coral Phosphate Prophylaxis Paste (CPPP), NuPro fine (NPF), NuPro medium (NPM), Prophydent (PD), Rembrandt Polishing Paste (RPLP) and Rembrandt Prophylaxis Paste (RPXP). The SEM photographs were rated by independent evaluators according to surface roughness from the smoothest to roughest surfaces. The results of the evaluator ratings determined that the composite resin surface gets rougher with the use of any polishing paste. The smoothest to roughest surface by groupings were: (SF)-(RPLP)-(CULP, RC)-(RPXP)-(NPF, CPPP, PD)-(NPM).

Composite Resins↗
PubMed1985

Description of a faculty-student group practice.

Dental schools are facing budgetary constraints, decreasing clinic patient populations, and increasing competition. At the same time, there is a need to provide students a realistic educational environment. The Dental Practice Systems (DPS) Program is a partially self-supported student-faculty group practice designed to address these issues. All individuals appearing for care are accepted into the program. Services are delivered by vertical teams of faculty preceptors, in-house specialty consultants, dental and dental hygiene students, and appropriate auxiliaries. All third- and fourth-year students rotate through the program providing clinical services. In addition, fourth-year students are assigned specific managerial responsibilities. The DPS Program maximizes the use of auxiliaries and students, allowing each to function as autonomously as is consistent with good patient care.

Behavioral Sciences↗