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F Gan

Publications and source records attributed to F Gan.

9 recordsLinked to original sources
PubMed2001

Two novel procedures for automatic resolution of two-way data from coupled chromatography.

In order to deal with the problem of simultaneous qualitative and quantitative analysis of the complicated samples in analytical chemistry, two novel procedures for automatic resolution of two-way data from coupled chromatography were developed in this work. The first can be used to determine automatically the number of components involved in a certain peak cluster. The second method is an iterative resolution procedure named the stepwise key spectrum selection which has also been developed to resolve automatically the spectra and chromatographic profiles of every component in the peak cluster investigated. The results obtained from simulated and real data show that the proposed methods perform fairly well.

Journal Article↗
PubMed2001

Library search of mass spectra with a new matching algorithm based on substructure similarity.

A new matching algorithm for library searches of mass spectra is presented in this paper. The algorithm is based on the substructure similarity of substances. It emphasizes m/z positions rather than abundance values. 32 spectra, whose corresponding molecular weights are less than 200, were randomly selected from a mass library of 61,993 spectra and taken as targets of library search to illustrate the availability of this algorithm. The results show that the algorithm is better than the one built in a commercial instrument when there is no spectrum of the unknown in the library but there are similar ones.

Journal Article↗
PubMed2001

An improved optimization strategy and its application to clustering analysis.

In this paper, a new optimization strategy is put forward which locates as many potential unimodal regions as possible in the search space. The potential optima can be further explored by a global optimization method for searching in the identified unimodal regions. The proposed strategy was evaluated by the optimization of test functions. The results obtained by this approach are comparable with those achieved by variable step size generalized simulated annealing (VSGSA) and a genetic algorithm (GA). Finally, we used this strategy in a clustering analysis of a tobacco data set.

Algorithms↗
PubMed1993

Single cell clone from culture cells of Panax ginseng.

The plating efficiency of a single cell clone from culture cells of Panax ginseng can be influenced by the nutritional compositions of plating medium. In order to promote the growth of single cells, it was essential to establish an appropriate ratio between 2,4-D concentration and KT concentration in the MS medium. The best combination of these two hormones was 1.5 mg/l and 0.5 mg/l, respectively. Considering the formation of cell clones, the suitable NH2NO4 concentration and CaCl2.2H2O concentration should be 400 mg/l and 750 mg/l, respectively. The plating efficiency can also be effectively increased when the plating medium was suitably supplemented with succinic acid, arginine, vitamins, etc. In optimization of the nutritional compositions of the medium, the plating efficiency was increased 2.34 times against the control. The cells suspended for 12 days were most favorable for the formation of cell clones. Different plating efficiency was obtained with different cell plating density. There was a low plating efficiency when the cell plating density was lower than 4 x 10(3) cells/ml.

Amino Acids↗
PubMed1992

Metabolic regulation of pigment formation of Onosma paniculatum cultured cells.

The pigment yields of Onosma paniculatum callus and suspension cultured cells were increased 5.5 and 8.1 fold of that of the control respectively, when 10(-5) M of copper ion was added into the medium. The pigments synthesis was also stimulated greatly when 10(-5) M of L-phenylalanine was added into the medium and the cells were cultured for twenty-one days. The maximum yield of pigments was obtained when 10(-6) M of ascorbic acid was added to the medium.

Ascorbic Acid↗
PubMed1989

Establishment of a group of murine leukemic cell lines and investigation of their biological characteristics.

An in vitro-vivo technique for establishment of cell lines on murine leukemia has been developed. Using this method, suppressive T lymphoblastic leukemia L7811-85, L7212-85, non-T, non-B lymphocytic leukemia L1210-86, B lymphocytic leukemia P 388-86 and Friend erythroleukemia FLCL cell lines have been established. Incidence of leukemia with these cell lines was 100%. Along with the increase of generations of cell lines, cell growth accelerated, generation time shortened and cloning efficiencies rose. A following up electron microscopic observation on L7811-85 and L7212-85 showed that the virus particles were "A" particles in original cells. When they became cell lines in vitro, virus particles increased and transformed into typical "C" particles with budding. An inhibitory activity relevant to leukemic cells on proliferation of leukemic cells has been observed in the supernatant of L7811-85 medium and was regarded as an "autocrine".

Animals↗