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F Gavini

Publications and source records attributed to F Gavini.

42 records · Page 3Linked to original sources
PubMedDate not supplied

Separation of Escherichia adecarboxylata from the "Erwinia herbicola-Enterobacter agglomerans" complex and from the other Enterobacteriaceae by nucleic acid and protein electrophoretic techniques.

The species Escherichia adecarboxylata was examined for DNA relatedness to the "Erwinia herbicola-Enterobacter agglomerans" complex and to other members of the family Enterobacteriaceae. DNA-DNA hybridizations (nitrocellulose filter method) showed that strains received as E. adecarboxylata were highly related to each other (73-100% homology). Three strains of E. agglomerans and one strain of E. herbicola showed, respectively, 77, 96, 97 and 92% relatedness with the labelled DNA of E. adecarboxylata. Two groups (E2 and E3) of "atypical coliforms" previously described by Gavini et al. (1983) showed high reassociation values (76-79% and 80-89%, respectively) with E. adecarboxylata. Most of these strains produced similar or nearly identical protein electrophoregrams. All these strains were therefore classified in E. adecarboxylata. This taxon yielded hybridization values lower than 53% with the previously described phenetic or genetic groups belonging to or related to the "herbicola-agglomerans" complex and values lower than 64% with 56 other species of the Enterobacteriaceae. It was concluded that E. adecarboxylata is a species different from E. agglomerans and the other species of the family Enterobacteriaceae. A new definition of the species E. adecarboxylata is presented.

Bacterial Proteins↗
PubMedDate not supplied

[Rahnella aquatilis, a new member of the Enterobacteriaceae (author's transl)].

A DNA-DNA hybridization study was carried out to determine the taxonomic position of a new group of enterobacteria (group H2) previously studied by numerical taxonomy. All the strains of this group revealed relatively high reassociation binding ratios with the centrotype; 82% of the strains of the group showed more than 69% of reassociation with the centrotype. In spite of numerical taxonomy conclusions, there was no genetic relationship with the species Enterobacter cloacae (higher reassociation binding ratio: 37%). No significant genetic relationship with the other groups of enterobacteria was found. Rahnella aquatilis was defined from phenotypic and genetic data. The strain 133 (CIP 78-65) is proposed as type strain of the species.

DNA, Bacterial↗
PubMedDate not supplied

[Immunological study of glyceraldehyde-3-phosphate dehydrogenase in Enterobacteriaceae; taxonomic value].

The antigenic structure of glyceraldehyde-3-phosphate dehydrogenase of the most representative Enterobacteriaceae species were compared with an antiserum to Escherichia coli glyceraldehyde-3-phosphate dehydrogenase. The results of the immunodiffusion experiments were confirmed and specified by micro-complement fixation studies. They demonstrated a total immunological identity between the E. coli enzyme and the enzymes of Alcalescens-dispar and the Shigella species, a marked relatedness of the Salmonella species enzyme and a more or less significant relation of the enzymes of the other Enterobacteriaceae species. Moreover, the micro-complement fixation had the same sensitivity and a better selectivity than the DNA/DNA hybridizations. The results show that, like DNA polymerase, this enzyme has evolved more slowly than the other enzymes studied at this time.

Complement Fixation Tests↗
PubMedDate not supplied

[Study of the species "Enterobacter gergoviae" by DNA/DNA hybridization (author's transl)].

A DNA/DNA hybridization study was carried out with 13 strains of Enterobacter gergoviae and 123 strains of Enterobacteriaceae, Mostly Enterobacter and Klebsiella species. E. gergoviae strains were very highly related (relative binding ratios: 88-96%) to the type strain CIP 76-01, as previously reported by Brenner and co-workers. No close genomic relationship was found with other species of Enterobacter and Klebsiella (mean relative binding ratio: 39%) and other Enterobacteriaceae.

DNA, Bacterial↗
PubMedDate not supplied

[Taxonomic positions of H2S-Enterobacteria in relation to the genus (Citrobacter) (author's transl)].

This work studies the classification, by numerical procedure, of 111 strains belonging to the family Enterobacteriaceae. Their taxonomic position is discussed, in relation to the genus Citrobacter, Levinea, Enterobacter, to which some appear to be closely associated. The study is based on the usual biochemical and nutritional characters as shown by the utilisation of substrates tested as sole sources of carbon and energy. Six hierarchical classes can thus be defined. Their degree of affinity with members of the Enterobacteriaceae groups or genera is discussed. The diverse origins of the strains examined (human, soil, aquatic) are certainly the cause for the individualization of the new classes.

Alcohols↗