PubMed Health⌕ Search

Biomedical subjects

F Guignier

Publications and source records attributed to F Guignier.

11 recordsLinked to original sources
PubMed1992

[Standardization trial of ABO-Rh(D) blood typing using a U-microplate].

This study reports the results of ABO-Rh (D) typing in microplate according to a suggested protocol. 35,532 blood typings were performed by 13 laboratories, compared to usual technics. This work has proved the feasibility in routine of this protocol in order to identify the A, B, D and weak antigens. However the difficulties in detecting some weak variants reveal the interest of standards for immuno-haematology reagents, to apply in the microplate technology.

ABO Blood-Group System↗
PubMed1991

[Preparation of leukocyte-depleted human platelet concentrates by centrifugation and filtration of a pool of sterilely connected buffy-coats].

We describe a new method for the preparation of standardised therapeutic doses of leukocyte depleted platelets. The first step is to remove the buffy-coat from whole blood units drawn on triple Siamese ACD/SAGM bags (Maco-Pharma) by means of a Compomat (NPBI). The second step is to connect (SCD Haemonetics) six buffy-coats and one plasma to a special kit (Maco-Pharma) including a PALL PL 100 filter; after centrifugation, the supernatant platelet concentrate is extracted, filtered and recovered in a 2 litre TOTM PVC bag. The volume, the number of platelets and leukocytes of these pools are measured. A comparison of these parameters is made with therapeutic doses prepared in the same way without filtration. Besides, pH measurements up to the 6th day of storage and bacteriological checks are carried out. The results show: no platelet loss related to filtration; a synergy between the preparation process out of buffy-coats and the filtration: so each dose contains less than 10(6) leukocytes; a good pH level allowing the storage for five days as it is associated to the bacteriological safety of the functionally closed system. This technique makes it possible to transfuse only leukocyte depleted platelet concentrates. In addition, it offers new prospects for standardisation and quality improvement.

Blood Transfusion↗
PubMed1990

Serological characterization of murine monoclonal antibodies directed against acquired B red cells.

Balb/c mice were immunized with acquired B red cells. Twelve clones specific for acquired B red cells were obtained from two fusions. A detailed investigation of three clones is reported here. These antibodies appear to be directed to the B-like epitope since they are inhibited by galactosamine and fail to react after acetylation of red cells. E 231 is an example of a series of antibodies closely specific for acquired B red cells which can be useful in elucidating some AB0 typing problems. E 167 and F 47 showed a cross-reactivity with A1 red cells and a synthetic A trisaccharide. No affinity for B antigen could be demonstrated for any of the antibodies.

ABO Blood-Group System↗
PubMed1989

[Leukocyte removal in a closed system of human red-cell concentrates: a technic coupled with the automated extraction of the buffy coat using a sterile connector from a filtration kit].

A technique, integrally run in a closed system, for leucocyte depletion of human red cell concentrates is described. It associates two complementary processes: buffy-coat removal and filtration. The first step is carried out with an automated system for blood component preparation (Compomat, NPBI); its efficiency is improved by a custom made blood collection set with ACD anticoagulant solution in the primary bag. The second step is simplified by a filtration kit requiring only one sterile connection for operation (SCD 312, Dupont de Nemours) and allowing a standardised rinsing of the filter. Quality control of 33 units so prepared shows principally: --an intensive leuko-depletion (4 logs) enabling leukocyte contamination to be kept below 10(6) per unit; --a moderate red cells loss (15 ml for the first step and 20 ml for the second one). This technique provides a permanently available and very pure blood component. Moreover it offers new potential for standardisation and mastery of quality control.

Blood Cell Count↗
PubMed1989

[Viability of human red blood cells preserved for 35 days after leukocyte depletion (in vitro study)].

24 leukocyte poor red cells concentrates (L.P.R.C.) were prepared by sterile connection of a leucocyte filter between the primary bag and the SAGM bag of a blood unit after centrifugation. Their quality was followed up to 42 days by means of a panel of tests including, ATP and 2,3-DPG levels, hemolysis, plasma potassium, lactate and glucose, and counts of the microaggregates. 24 standard units acted as a control group. Results showed better preservation of LPRC and especially less hemolysis, higher ATP levels and at least equal oxyphoric capacity (explored by 2,3-DPG). Microaggregate formation was dramatically reduced and bacteriologic checks (48 at day 25 and 48 at day 42) were all negative. Leucocyte depletion appears as a new way to improve functionality of erythrocytes during storage in the SAGM medium. 35 days shelf life will allow this blood product to be more available and its preparation more standardised.

2,3-Diphosphoglycerate↗
PubMed1988

Comparison between a solid-phase low-ionic-strength solution antiglobulin test and conventional low-ionic-strength antiglobulin test: assessment for the screening of antierythrocyte antibodies.

A solid-phase low-ionic strength salt antiglobulin test (LISS-SPAT) has been developed using a microplate coated with dried sera as a solid phase. Before coating, the in vitro C3d fragment generation was activated by adding heat-aggregated immunoglobulin. The LISS-SPAT was compared with low-ionic strength conventional antiglobulin test (LISS-AGT) and also with a test using polybrene or papain microplates. When detecting the IgG and IgM antierythrocyte antibodies the reaction was developed in the same way in LISS-SPAT and LISS-AGT. In routine work, the LISS-SPAT provides a fast, reliable, handy and inexpensive screening of antibodies. This method appears to be an additional method to the papain and polybrene tests in microplates.

Blood Group Antigens↗
PubMed1985

Outbreak of respiratory syncytial virus in France.

A report is given of an outbreak of respiratory syncytial virus infection in a neonatal unit in France. Twenty-three of 32 infants were infected (72%) despite infection control procedures. Prophylactic administration of non-specific gamma globulins was associated with a significant decrease in infection rate (p less than 0.05). The administration of transfer factor to infected infants was also associated with a significantly lower rate of severe respiratory diseases (p less than 0.05).

Disease Outbreaks↗
PubMed1977

[Radioimmunologic determination of the thyrotropin releasing hormone].

We describe the preliminary steps for a radio-immunoassay of Thyrotropin Releasing Hormone (TRH). Rabbit antiserum at dilution 1 : 10 000 is used with radioiodinated TRH (125I). We are able to assay from 5 to 1 000 pg unlabeled TRH with an intraassay reporducibility varying from 7 to 4 % and the lowest detectable amount in this system is 10 pg TRH. TRH mean and standard deviation in normal subjects are 136,9 and 25,3 pg/ml.

Female↗
PubMedDate not supplied

[Assessment and value of the polybrene microplate test for study and identification of irregular anti-erythrocyte antibodies].

We have adapted Lalezari's manual polybrene test for use with microplate technology for screening and identification of anti-erythrocytes antibodies with a view to future automation. The technical conditions have been standardized, firstly by using a programmable centrifuge and a sequential shaking, secondly by using a preservative medium for panel after dispensing onto microplates. This methodology has been run in parallel with papain test and LIS indirect antiglobulin test: 7,000 screenings have been performed and their results are considered here. Our results are comparable to those described for automatic and manual techniques. The polybrene-microplate test affords a fast, reliable, handy and inexpensive means of screening and identification for irregular antibodies. It appears as an additional method for enzymatic tests in microplate. An antiglobulin test can be carried out after negative tests.

Antibodies↗