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Biomedical subjects

F H Bach

Publications and source records attributed to F H Bach.

At least 397 records · Page 22Linked to original sources
PubMed1978

Sensitization of lymphocytes against pooled allogeneic cells. I. Generation of cytotoxicity against autologous human lymphoblastoid cell lines.

Lymphocytes sensitized in vitro to a pool of X-irradiated allogeneic normal lymphocytes from 20 individuals develop cytotoxic activity for autologous human lymphoblastoid cells (LCL). Whereas pool sensitized T lymphocytes lyse autologous LCL cells, they fail to lyse autologous B-enriched or T-enriched normal target cells nor autologous phytohemagglutinin (PHA) blasts. In contrast to pool sensitization, stimulation with normal cells of single allogeneic individuals rarely led to development of cytotoxicity against autologous LCL cells. We conclude that human Epstein-Barr virus transformed LCL cells express target antigens cross-reactive with allogeneic target antigens expressed on normal cells and that sensitization with a pool of allogeneic cells is an effective means of generating effector cells directed against autologous abnormal cells.

B-Lymphocytes↗
PubMed1978

Pool-priming: A means of generating T lymphocytes cytotoxin to tumour or virus-infected cells.

It is proposed that cells cytotoxic for autologous or syngeneic tumour cells can be produced in vitro by sensitisation of lymphocytes to a pool of normal cells from 20 unrelated individuals. Restriction on molecules that can serve as cytotoxic determinants (C.D.), may explain why a random pool of cells from 20 people could present essentially all of the determinants (C.D.) may explain why a random pool of occur because of extensive cross-reactivity of antigens on tumour cells with normal alloantigens present in the stimulating pool.

Antigens, Neoplasm↗
PubMed1978

Alloantigen-induced enhancement and suppression of human cytotoxic lymphocyte responses to autologous lymphoblastoid cell lines.

In vitro sensitization of human lymphocytes to autologous lymphoblastoid cell lines (LCL) has been shown to give rise to cytotoxic lymphocytes capable of lysing autologous as well as allogeneic LCL cells. However, allogeneic LCL cells were found to be markedly less effective than autologous LCL cells in terms of generating lymphocytes capable of lysing autologous LCL cells. The addition of allogeneic LCL cells or allogeneic normal lymphocytes to a mixture of responding lymphocytes and X-irradiated autologous LCL cells suppressed the generation of cytotoxic lymphocytes against autologous LCL cells. Furthermore, suppressor T cells generated in allogeneic mixed leucocyte culture (MLC) and supernatants from MLC likewise decreased the generation of cytotoxic lymphocytes to X-irradiated autologous LCL cells. In contrast to the findings that alloantigens suppress the generation of cytotoxicity of X-irradiated autologous LCL cells, which ordinarily induce strong cytotoxic responses, were the findings that allogeneic stimulating cells and supernatants from MLC enhanced cytotoxic responses to autologous ultraviolet light or extensively heat-treated LCL cells that induce weaker cytotoxic responses. The possible mechanisms whereby alloantigens enhance or suppress cytotoxic responses to autologous abnormal cells and the implications of these findings are discussed.

Cell Line↗
PubMed1978

Association of mouse major histocompatibility and Rauscher murine leukaemia virus envelope glycoprotein antigens on leukaemia cells and their recognition by syngeneic virus-immune-cytotoxic T-lymphocytes.

Physical association was measured between MLV gp70, the envelope glycoprotein of Rauscher murine leukaemia virus (R-MLV), and serologically defined H-2 antigens on the surface of R-MLV transformed C57BL/6 (H-2DbKb) mouse leukaemia cells (RBL-5A). Capping and patching with antisera against H-2Db caused specific co-capping and co-patching of R-MLV gp70 antigens as seen by fluorescence microscopy. Despite the physical proximity of R-MLV gp70 and H-2Db antigens, high titre alphaR-MLV gp70 sera had no effect in blocking syngeneic T-lymphocyte mediated cytolysis of RBL-2A cells whereas alphaH-2Db sera were effective.

Animals↗
PubMed1977

Selective activation of human suppressor cells by a nonproliferative stimulus.

The eliciting of suppressor cell activity by heat-treated stimulating cells demonstrates that suppression can be induced by an antigenic but non-effective mixed lymphocyte culture (MLC) stimulus in vitro. Simultaneous or prior MLC or cytotoxic T lymphocyte activity is not required for alloantigen-induced suppressor cell activation.

Antigens↗
PubMed1977

Frequency of W4 and W6 antigens in perennial, nonallergic asthmatics.

The frequency of W4 and W6 antigens was determined in 39 perennial, nonallergic asthmatics and 53 normal controls. The data indicated that the frequency of asthmatics homozygous for W4 or W6 was not different from the unrelated control population. Hence, our data dispute the hypothesis that intrinsic asthma is an autosomal-recessive disease associated with the W6 antigen.

Asthma↗
PubMed1977

Mixed leukocyte culture incompatibility index for donor-recipient selection in kidney transplantation.

The mixed leukocyte culture test has been applied to selection of histocompatibility non-identical donor-recipient pairs for renal transplantation. The clinical course of transplant recipients who have histocompatibility mismatches but low mixed leukocyte culture stimulation is similar to that of mixed leukocyte culture and histocompatibility identical recipients. Living donor-recipient pairs with high mixed leukocyte culture stimulation had no better graft survival than cadaver recipients. An incompatibility index derived from mixed leukocyte culture may aid in the selection of satisfactory non-identical living related donors and may help avoid use of immunologically unsatisfactory living donors.

Cadaver↗
PubMed1977

A T-cell marker in mouse fibroblast x T-lymphocyte somatic cell hybrids.

Continuous hybrid cell lines have been generated by the fusion of allogeneically primed murine T lymphocytes with mouse L-cell-derived fibroblasts of the line 613. The resulting hybrid clones express one or more T-cell-specific surface components identifiable by radioiodination of intact cells followed by NP-40 solubilization of membrane proteins and sodium dodecyl sulfate polyacrylamide gel electrophoresis. These markers are stably expressed by the hybrid lines after several months of passage.

Animals↗