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Biomedical subjects

F H Jaartsveld

Publications and source records attributed to F H Jaartsveld.

At least 19 recordsLinked to original sources

Incidence of paratuberculosis after vaccination against M. paratuberculosis in two infected dairy herds.

Vaccination against paratuberculosis of all newborn animals has been performed since April 1984 in two dairy herds with a high incidence of clinical cases of paratuberculosis, using a vaccine containing heat-inactivated M. paratuberculosis in a water/mineral oil emulsion. Animals slaughtered between April 1984 and January 1991 were included in the study. Histology, bacterioscopy and culture on Smith and modified Löwenstein-Jensen media were performed using jejunum, ileum and draining lymph nodes. The animals present on the farm in April 1984 constituted a retrospective non-vaccinated group, giving an indication of the initial infection rate. After vaccination, the percentage of animals culled for clinical paratuberculosis decreased significantly (7.8 to 1.8%; P < 0.005), as did the percentage of animals with positive histology (11.8% to 5%). The incidence of infected animals, defined by positive results in histology and or bacterioscopy and/or culture, however, increased from 21.8% to 25.9%.

Animals

[Doxycycline poisoning in veal calves].

Several veal calves that had received doxycycline died suddenly. Post mortem examination revealed pulmonary oedema, myocardial degeneration, and myocarditis. It is possible that hitherto unknown interactions between doxycycline and other compounds may have played a role.

Animals

[Rhodococcus equi infection in a goat].

Enterotoxaemia caused by Clostridium perfringens was diagnosed in a goat affected with severe enteritis which was found to be present at autopsy. Lesions bearing a resemblance to tuberculosis, caused by a Rhodococcus equi infection, were observed in the liver and lungs. This bacterium is constantly isolated from pulmonary abscesses in foals affected with pyemia. Similar cases of infection in goats were previously reported in the literature.

Abscess

[Contagious equine metritis in The Netherlands].

Contagious Equine Metritis (CEM) was detected in the Netherlands for the first time in 1987. A total number of five mares (Dutch saddle-horse) were infected in three separate outbreaks. The origin of the infection could not be determined in any of the cases. As the isolates of the causal organism, Taylorella equigenitalis, showed auto-agglutination, diagnosis was difficult. Therefore, an indirect immune fluorescence test as used to diagnose the second isolate. Five strains were isolated, which all were resistant to streptomycin. The prevalence of CEM since 1981 is summarised. The importance of CEM in horse-breeding is discussed.

Animals

[Listeria mastitis in cattle].

Cases of human infection caused by Listeria and resulting from the consumption of dairy produce contaminated by Listeria are referred to in the literature. A case of mastitis in cattle, caused by Listeria monocytogenes type 1/2a, is reported. The result of treatment of the infected quarters with penicillin was not satisfactory so far. Several cases of short-lived excretion of Listeria bacteria in the milk and carriers not showing any symptoms are referred to in the literature, whereas cases of prolonged mastitis caused by Listeria, marked by abnormal milk, increased cell counts and reduced production are not reported.

Animals

Effect of glucocorticoids on cows suspected of subclinical infection with M. paratuberculosis.

Glucocorticoids were administered to 10 heifers suspected of subclinical infection with Mycobacterium paratuberculosis. Three animals remained untreated. M. paratuberculosis was isolated from the internal organs of 2 animals after this treatment but not from any of the control group. Delayed type hypersensitivity and lymphocyte reactivity towards Johnin and purified protein derivates of M. avium and M. bovis were depressed. A sharp increase in total leucocyte count, due to an increase in neutrophil numbers, occurred. In the three untreated animals these parameters did not change during the experiment. A decrease of specific immunological reactivity towards M. paratuberculosis occurred, but not to such an extent that clinical disease developed.

Animals

[Mycoplasma bovis arthritis in a herd of dairy cattle].

Cases of severe arthritis due to M. bovis in a herd of dairy cattle are reported. M. bovis was also isolated from the mammary tissues and lungs of one of the lame animals at autopsy; this animal did not show any symptoms of respiratory disease or mastitis. M. bovis was detected in milk samples of eight animals in which mastitis was present or absent, though not in any of the animals affected with arthritis. Antibiotic therapy failed to produce permanent results in the lame animals. Ultimately, spontaneous recovery occurred in these cases. M. bovis was no longer isolated following the short period during which lameness was present.

Animals

[Diagnosis of paratuberculosis in the preclinical stage].

Those tests which are common practice in the diagnosis of Johne's disease in the Netherlands, namely the intradermal johnin hypersensitivity test, the complement fixation test and microscopic examination as well as examination of the faeces by culture, were carried out in twenty-one cattle from four herds infected with Johne's disease. In addition, the johnin lymphocytic stimulation test was performed. A number of animals of various ages, which were tested, showed positive johnin hypersensitivity tests prior to the beginning of the 1982 grazing period (Table 1). M. paratuberculosis was isolated from five of these twenty-one animals after death. Six animals showed a positive complement fixation test one or several times; M. paratuberculosis was isolated from four of these animals after death. On the other hand, M. paratuberculosis was isolated from an animal in which this hypersensitivity test had been constantly negative. The other animals from which M. paratuberculosis was isolated, showed positive johnin hypersensitivity tests in some and negative tests in other cases. Not a single shedder of M. paratuberculosis was identified using microscopic examination of the faeces. When the faeces were examined by culture, one of the three animals found to be positive after death was identified. The animals from the suspected herds showed a higher average johnin stimulation index (SI) than did those from herds free from Johne's disease. However, when the LST was used, all carriers of M. paratuberculosis also were not detectable. In conclusion, it has to be stated that none of the tests studied in these cases removed all doubt as to whether the animals were or were not subclinically infected with M. paratuberculosis.

Animals

[Organizing mastitis control (author's transl)].

In view of the considerable losses caused by clinical, though particularly subclinical, mastitis, organized mastitis control is justified. Cell counts of herd milk samples are the most effective method so far available in detecting herds in which mastitis is a problem. Cell counts are primarily made to determine the quality of the milk delivered; secondly, cell counts are used in mastitis control. Using the same cell count in determining the quality and in detecting herds affected with mastitis is a difficult matter. Herds in which mastitis is a problem, are systematically detected in a limited number of provinces in the Netherlands. In mastitis control on a farm, particular attention is paid to: (1) hygiene on the farm (2) the house climate (3) special arrangements for maintenance of the milking plant (4) the procedure adopted in milking (5) teat dipping (6) dry cow therapy (7) culling chronically infected cattle. Because of mastitis in the cows, there may be an increase in bacterial counts due to streptococci causing mastitis in addition to cell counts in herd samples. These streptococci are perceptible in the form of small punctate colonies, so-called pin-points, in bacterial counts. Bacteria other than streptococci causing mastitis may also produce these pin-points. In recent years, the cell counts of herd milk samples showed a marked decrease in the southern Netherlands. This decrease was due in part to the large number of improvements in the milking apparatus. Moreover, intensive education by lectures, excursions and counselling of factories has also stimulated this favourable development. However, these effects are hard to measure. The continued reduction of the cell count in herd samples imparts confidence in the results of this mastitis control.

Animals

[A survey of mastitis control, based on the number of drug containers made commercially available in the Netherlands during the period from 1971 to 1977 (author's transl)].

The figures on the number of marketed short-acting and long-acting antibiotics for the control of mastitis reported by the industry convey an impression of the mastitis situation in the Netherlands. The numbers and proportions of quarters and animals treated with short-acting antibiotics as well as the number and proportions of dry cows are reviewed. The figures suggest that the number of animals treated for acute mastitis are gradually decreasing, whereas the number of cattle treated with long-acting antibiotics in the early part of the dry period continues to be fairly constant.

Animals

The cell count of milk in relation to milk yield.

Quarter-milk cell counts and milk yields from 1000 cows in the province of Noord-Brabant were obtained over a 12-month period. The data from 933 animals, mainly heifers, were used to examine statistically the relationship between cell counts, as a criterion for sub-clinical forms of mastitis, and milk yield. Losses in milk were assessed by quarter, by pairs of quarters and by cow. The conclusions were that the losses in milk due to sub-clinical forms of mastitis, as indicated by cell count, can be substantial. The existence of a compensating increase in milk yield from a healthy gland, in response to the loss of milk from a neighbouring quarter with raised cell count, could not be demonstrated.

Animals

[Drug residues in poultry (author's transl)].

The following microbiological tests: the Bacillus subtilis BGA (Bundes-Gesundheitsamt) test and Sarcina lutea test as well as the test bacteria Bacillus stearothermophilus var. calidolactis and E. coli were used to examine whether drug residues were detectable in broiler chickens during and after treatment with various drugs. With the exception of the occidiostat Esb3, residues were not found to be present using the two above tests. On the other hand, residues of a number of drugs were detected in some interior organs and faeces during treatment when the other test bacteria were used. Residues were no longer detected in the faeces, however, within twelve hours after treatment had been discontinued.

Animals

[Use of the milk ring test in herd samples (author's transl)].

Performing the milk ring test in herd samples from herds of approximately twenty dairy cows is an acceptable method in checking herds for brucellosis. Herds of approximately forty dairy cows or more are unsuited for this method of checking. For the above reasons, it is essential that the compulsory examination of the blood of aborting cows should be consistently carried out and that the larger herds in the threatened areas should be examined by performing the milk ring test in individual samples at regular intervals.

Animals