Biomedical subjects

F H Li

Publications and source records attributed to F H Li.

3 recordsLinked to original sources

PubMed1992

Analysis of antigenic polypeptides of Dane particles and antibody response ability of HBV infected subjects to PreS1 polypeptides.

We demonstrated the constitutive polypeptides (PP) of Dane particles employing Western Blot and investigated the antibody response ability of HBV infected subjects to PreS1 PP in comparison with other serum markers from HBV infected individuals. The results indicated that 1) the major reason for discrepant results may be related to the detergents used in the sample solutions and the degree of denaturation the samples had undergone; 2) there are 12 bands in the PAGE-graph of Dane particles. By Western Blot it was confirmed that 5 PP (P24, P27, P36, P39, P42) are derived from S-open reading frame (S-ORF), P21 is associated with C-ORF, P24-25 possesses some epitopes of Pol protein, and P45 and P76 express similar epitopes to human IgG and IgM; and 3) the prevalence of anti-PreS1 PP was 17.24% in the group of healthy persons following latent HBV infection, much higher than that of HBV infected patients (1.21%). The above findings imply that antibody response ability of the host to PreS1 PP is attributing to the outcome of HBV infection. It may play an important role in the elimination of the virus.

Blotting, Western↗

PubMed1991

[Investigation of anti-hepatitis C virus antibody in healthy blood donors in China: enzyme immunoassay and recombinant immunoblot].

281 serum samples from Chinese healthy blood donors (HBsAg negative and ALT normal) were tested for anti-HCV antibodies with ortho-HCV ELISA test system and Abbott HCV EIA diagnostic kits. The results were further confirmed with recombinant immunoblot assay (RIBA). Our research showed that the prevalence of anti-HCV antibody in this group was 2.1% (6/281), being higher than that in other countries and regions. It indicated that great attention should be paid to HCV infection in China. The reason of dark groundback and high false positive reactivity produced by the ELISA was discussed. So, the positive reactivity produced by ELISA should be confirmed by other specific method such as RIBA. Reexamination of the 281 serum samples with HBsAg AUSRIA II showed that HBsAg was positive in 7 and HBVDNA positive in one of them.

Blood Donors↗

PubMed1977

Inbreeding estimation from population data: models, procedures and implications.

Four different estimation procedures for models of population structure are compared. The parameters of the models are shown to be equivalent and, in most cases, easily expressed in terms of the parameters WRIGHT calls "F-statistics." We have estimated the parameters of each of these models with data on nine codominant allele pairs in 47 Yanomama villages, and we find that the different estimators for a given parameter all yield more or less equivalent results. F-statistics are often equated to inbreeding coefficients that are definid as the probability of identity by descent from alleles taken to be unique in some founding population. However, we are led to infer from computer simulation and general historical considerations that all estimates from genotype frequencies greatly underestimate the inbreeding coefficient for alleles in the founding population of American Indians in the western hemisphere. We surmise that in the highly subdivided tribal populations which prevailed until the recent advent of civilization, the probability of identity by descent for homologous alleles was roughly 0.5. We consider some consequences of working with the customary, much lower, estimates--0.005 to 0.01--if, on the time scale of human evolution, these represent only a very recent departure from the inbreeding intensity that prevailed before civilization.

Alleles↗