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F Hatey

Publications and source records attributed to F Hatey.

6 recordsLinked to original sources
PubMed1992

Gonadotropins induce accumulation of insulin-like growth factor I mRNA in pig granulosa cells in vitro.

Pig granulosa cells have been shown to synthesize insulin-like growth factor (IGF) I peptide in vitro, and this expression is regulated by gonadotropins via the cAMP pathway. By hybridizing an IGF I cDNA probe with total RNA isolated from pig granulosa cells cultured in vitro, we show that these cells contain two IGF I transcripts of about 0.9 kb and 9 kb in size. Treatment of the cells with gonadotropins (follicle-stimulating hormone, luteinizing hormone) or cAMP agonists (dibutyryl-cAMP, forskolin) induces an accumulation of the transcripts which can be abolished by transcriptional inhibitors, but not by translational inhibitors. We thus provide new evidence that pig granulosa cells are a site of IGF I synthesis, and we conclude that (1) gonadotropins increase IGF I mRNA levels; (2) the accumulation of IGF I mRNA results from an increased transcription; (3) the stimulation of IGF I gene transcription does not require ongoing protein synthesis; (4) these effects of follicle-stimulating hormone can be mimicked by cAMP agonists.

Animals↗
PubMed1979

Protein synthesis inhibition in rat liver by the mycotoxin patulin.

Patulin, a carcinogenic mycotoxin, inhibits in vivo and in vitro protein synthesis in rat liver. The in vivo inhibition culminates 5 h after toxin administration and reaches a maximum of 65% with regard to control values; a breakdown of polysomes is associated with the translational blockage. However in in vitro systems, the cellular fractions obtained from patulin-treated rats appear equally as active in protein synthesizing ability and as sensitive to the toxin action as those prepared from control animals. The in vitro inhibition by patulin is dose related. The postmitochondrial system is less sensitive than that functioning with isolated polysomes and pH 5 enzyme. This difference might be due to the presence of soluble factor(s) that counteract patulin action. We propose that the inhibition of protein synthesis by patulin may result from an interaction of the drug with active SH groups at the membrane level (amino acid transport, ion equilibrium) and/or at the cytoplasmic level (enzymes and factors involved in the translational process).

Amino Acids↗
PubMed1977

[Effect of patulin of RNA and protein synthesis in vitro].

Patulin, a mycotoxin isolated from cultures of Byssochlamis nivea, alters the transcriptional and translational processes. Using cell-free systems derived from procaryotic and mammalian cells, we have studied the mechanism of the mycotoxin-induced inhibition of in vitro RNA and protein syntheses.

Animals↗
PubMed1977

[Short term toxicity of ochratoxin A: some biochemical lesions in the rat (author's transl)].

The blood serum level of total proteins, urea, total lipids, cholesterol and glucose, and the excretion of nitrogen in the urine were estimated in control or treated rats. Male adult rats were given the toxin per os (0, 0.5, 1 or 2 mg/kg) during 10 days. An increase in the urinary volume was observed, with a decrease in the total nitrogen concentration. Blood total proteins and urea levels were higher than in control animals but total lipids and cholesterol levels dropped. Blood glucose concentration remained unaffected. These effects were evident even for the lowest dose and their intensity increased with the amount of toxin given to the animals. Statistical analysis showed significant differences between the results obtained in control animals and those obtained in animals treated with either 2 mg/kg or--except for blood urea--1 mg/kg.

Animals↗