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Biomedical subjects

F Hein

Publications and source records attributed to F Hein.

10 recordsLinked to original sources
PubMed2000

Cloning and characterization of eight cytochrome P450 cDNAs from chickpea (Cicer arietinum L.) cell suspension cultures.

Eight different P450 sequences were isolated from a cDNA library derived from cultured chickpea cells (cultivar ILC3279) elicited with a Phytophthora sojae (formerly megasperma) elicitor (Pmg-elicitor) by screening with heterologous and homologous probes. Screening with CYP73A1 from Helianthus tuberosus yielded several clones with one identical sequence. A full-length clone could be isolated and this sequence was assigned CYP73A19. Heterologous expression in yeast confirmed that CYP73A19 is the trans-cinnamic acid 4-hydroxylase of chickpea. Screening with a CYP81E2 polymerase chain reaction fragment from chickpea yielded a CYP81E2 full-length sequence and two almost identical CYP81E3 sequences, differing in only 16 out of 498 amino acids; both share more than 85% homology with the isoflavone 2'-hydroxylase from licorice (Glycyrrhiza echinata L.). Using CYP93A1 as a probe, it was possible to isolate a full-length member of the CYP93 family, CYP93C3, that shares more than 80% homology with isoflavone synthase from soybean. In addition, partial sequences CYP81E3, CYP81E4 and CYP81E5 were also found in this screening. The use of a CYP82A2 probe derived from BAC F10N7 from Arabidopsis thaliana yielded only one sequence, CYP76F1. Rescreening with CYP81E4 and CYP81E5 did not result in the isolation of any new P450 sequences. Northern blot experiments revealed that all but the CYP76F1 are induced rapidly and transiently in cell cultures upon elicitor treatment.

Journal Article↗
PubMed2000

Removal of a ruptured, detached, and entrapped angioplasty balloon after coronary stenting.

Entrapment of balloon catheters after coronary angioplasty or stent implantation is a complication during these procedures. We describe the removal of a ruptured angioplasty balloon which had become disconnected from the proximal shaft of the balloon catheter and entrapped after coronary stenting by using a 4 mm Amplatz "Goose Neck" Microsnare retrieval system (Microvena, White Bear Lake, Minnesota). The retrieval was accomplished without any complications. Retrieval systems should be on hand in all interventional catheterization laboratories.

Angioplasty, Balloon, Coronary↗
PubMed1988

Expression of Met-(-1) angiogenin in Escherichia coli: conversion to the authentic less than Glu-1 protein.

A method for obtaining authentic human angiogenin utilizing an Escherichia coli recombinant expression system is described. A synthetic gene encoding angiogenin was placed into a vector for direct expression under the control of a modified E. coli trp promoter. The protein was produced by the bacteria in an insoluble form and purified to homogeneity by cation-exchange and reversed-phase HPLC following reduction/solubilization and reoxidation. The protein isolated was identified as Met-(-1) angiogenin by amino acid analysis and tryptic peptide mapping; the latter demonstrated that all three disulfide bonds had formed correctly. Both the enzymatic and angiogenic activities of the Met-(-1) protein were equivalent to those of native angiogenin. A Met-(-1) Leu-30 derivative of angiogenin was also isolated and found to be fully active. Conversion of Met-(-1) angiogenin to the authentic less than Glu-1 protein was achieved by treatment with Aeromonas aminopeptidase under conditions in which the new N-terminal glutamine readily cyclizes nonenzymatically. This aminopeptidase treatment may have more general applicability for removal of undesirable N-terminal methionine residues from foreign proteins expressed in bacteria.

Amino Acid Sequence↗
PubMed1987

Chemoenzymatic synthesis of genes encoding medium-sized polypeptides by use of only one synthetic oligonucleotide.

A novel strategy for the synthesis of genes encoding medium sized polypeptides from only one synthetic oligodeoxynucleotide is outlined. A 140-mer oligodeoxynucleotide forming a hairpin structure at its 3'-end has been synthesized and successfully used in the construction and cloning of a gene coding for salmon calcitonin-gly(33). Employing this "one oligonucleotide - one gene" approach the manual work required for oligodeoxynucleotide synthesis is reduced to a minimum.

Amino Acid Sequence↗
PubMedDate not supplied

Genes expressed in Ascochyta rabiei-inoculated chickpea plants and elicited cell cultures as detected by differential cDNA-hybridization.

In response to the exogenous application of elicitors and attempted invasion by pathogens, plants exhibit a wide range of defense reactions. To understand the defense mechanisms at the level of gene activation and deactivation, differential screenings were performed to isolate cDNA clones which are differentially expressed in pathogen-inoculated resistant chickpea plants and elicitor-treated cell cultures. A plenty of genes were isolated and arranged in 5 groups, namely defense-related pathways, signal transduction pathways, regulation of gene expression, catabolic pathways and primary metabolism. Most of these genes were activated although several genes were also found to be suppressed. We discuss the plausible functions of cDNA products in plant defense responses. The cDNAs provide a variety of tools to investigate molecular mechanisms of defense responses and clearly reflect the massive genomic and metabolic changes which occur during manifestation of antimicrobial defense.

Ascomycota↗
PubMedDate not supplied

Early elicitor-induced events in chickpea cells: functional links between oxidative burst, sequential occurrence of extracellular alkalinisation and acidification, K+/H+ exchange and defence-related gene activation.

Elicitation of cultured chickpea (Cicer arietinum L.) cells stimulates a signal transduction pathway leading to several rapid responses: (1) oxidative burst, (2) extracellular alkalinisation, (3) extracellular acidification, (4) transient K+ efflux, and (5) activation of defence related genes all within 2 hours. Induced genes are encoding acidic and basic chitinases, a thaumatin-like protein and isoflavone reductase. All these elicitor-induced responses are inhibited by the Ser/Thr protein kinase inhibitor staurosporine and the anion channel blocker anthracene-9-carboxylic acid but stimulated by the Ser/Thr protein phosphatase 2A inhibitor cantharidin. The oxidative burst leads to a transient extracellular H2O2 accumulation which seems to be preceded by O2- production, indicating dismutation of O2- to H2O2. The oxidative burst is accompanied by transient alkalinisation of the culture medium which is followed by long-lasting extracellular acidification. An 80 percent inhibition of the alkalinisation after complete inhibition of the H2O2 burst with diphenylene iodonium indicates that the elicitor induced increase of extracellular pH is mainly based on a proton consumption for O2-dismutation. A simultaneous deactivation of the plasma membrane H+-ATPase during oxidative burst and extracellular alkalinisation is also suggested. The elicitor-stimulated extracellular acidification is inhibited by the plasma membrane H+-ATPase inhibitor N, N'-dicyclohexylcarbodiimide assuming a reactivation of the H+-ATPase 25 min after elicitation. Extracellular acidification seems not to be necessary for elicitor-induced activation of defence related genes. Opposite modulation of K+ and proton fluxes after elicitation and/or treatment with the H+-ATPase effectors fusicoccin or N, N'-dicyclohexylcarbodiimide indicate that the elicitor induced transient K+ efflux is regulated by a K+/H+ exchange reaction.

Anthracenes↗
PubMedDate not supplied

A summer institute on computer applications for nursing management: background, curriculum, and evaluation.

Nursing managers are faced with a growing number of computer applications for nursing management, yet they may lack the educational preparation to assist them in using these technologies for problem-solving and decision-making. This article describes a Summer Institute on informatics applications for nursing management taught by an international and multidisciplinary team of faculty members, and offered at the University of Limburg (Maastricht, The Netherlands). A discussion of professional, scientific, and educational issues serves as the foundation for curriculum content and instructional format. Evaluation data from both offerings are reviewed and underscore the professional relevance and didactic quality of the Summer Institute. The Summer Institute is presented as a possible model of continuing education in computer applications for nursing management transferable to Western European and North American countries.

Computer User Training↗