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Biomedical subjects

F Iglesias

Publications and source records attributed to F Iglesias.

15 recordsLinked to original sources
PubMed2001

Chemotaxis, viability, and labeling stability of leukocytes labeled with (99m)Tc-exametazime stabilized with methylene blue.

UNLABELLED: The in vitro viability, chemotaxis, and labeling stability of leukocytes labeled using (99m)Tc-exametazime stabilized with methylene blue were evaluated and compared with those obtained using nonstabilized (99m)Tc-exametazime. METHODS: Two identical leukocyte populations, from 30 healthy donors, were labeled simultaneously using freshly prepared and 2-h-old stabilized (99m)Tc-exametazime. The following quality control techniques were performed on each labeled leukocyte sample: eosin Y staining, chemotaxis radioassay, and labeling stability at 2 h after labeling. RESULTS: Eosin Y staining showed a cell viability of at least 98% in all samples, without a significant statistical difference between the populations. Chemotactic indices obtained with leukocytes labeled with freshly prepared, unstabilized (99m)Tc-exametazime were statistically greater than those obtained using (99m)Tc-exametazime stabilized with methylene blue (z = 2.41; P<0.02). Labeling stability at 2 h after labeling was the same for both populations. CONCLUSION: The use of (99m)Tc-exametazime stabilized with methylene blue for leukocyte radiolabeling does not affect either cell membrane integrity or labeling stability but can cause a decrease in the cell chemotactic capacity that discourages its clinical use.

Cell Survival↗
PubMed2000

[Labelling of ceftizoxime with 99mTc].

AIM OF THE STUDY: A new method to label antibiotics with 99mTc, using a third generation, wide spectrum cephalosporine (ceftizoxime), is presented. MATERIAL AND METHODS: 2.5 mg of ceftizoxime, 6.25 mg sodium dithionite in sodium bicarbonate buffer (pH=11.4) as a reductor agent, and 300 MBq of 99mTc were used. The final pH was adjusted to 8.4 with NaOH 0.1N. Radiochemical purity was assessed using ITLC-SG/MEK, ITLC-SG/0.9% NaCl and reverse phase HPLC. Biologic activity was assessed with the agar diffusion technique soaked with E. Coli in disks containing 10.5 microg labelled and non-labelled antibiotic. The maintenance of antibiotic activity was assessed by the diameter of the inhibition halos measured after 24 hours of incubation at 37 degrees C. RESULTS: The labelling efficiency was 94.9 +/- 2.4% (n = 20) and the complex was stable up to 6 h post-labelling. The HPLC chromatography showed five peaks: two of them at 1.7-2.0 min, which corresponds to the ceftizoxime derived product (82%-83% of the absorbance and about 90% of the radioactivity) and a third one at 4.7 min which corresponds to the intact ceftizoxime (6% of absorbance). The remaining two peaks appeared at 3.8 and 6.7 min and represented about 7%-8% of the absorbance. The antibiotic activity of the ceftizoxime-derived compound (CDC) was 83% of the unlabelled one. CONCLUSIONS: 1) The labelling method of ceftizoxime described causes a modification in its structure. 2) The 99mTc binds to the newly formed compound with high labelling efficiency and an acceptable shelf life stability. 3) The 99mTc-CDC maintains 83% of the original antibiotic activity and could be used for the detection of in vivo infection.

Ceftizoxime↗
PubMed1999

Irreducible phalangeal fracture in a child due to flexor tendon entrapment.

A 4-year-old girl sustained a longitudinal shaft fracture of the proximal phalanx of the ring finger. The fracture was irreducible by closed manipulation and required open reduction. The fragments were separated by both flexor tendons near the level of Camper's chiasma, which caused a "pinching" phenomenon when manipulative reduction was attempted. The case presented reveals that an irreducible fracture should be suspected in hyperextension injuries with severe angulation when rubbery resistance is noted at the time of closed manipulation.

Bone Wires↗
PubMed1998

[Effective renal plasma flow with I-131-hipuran: evaluation of a method with four extractions].

The aim of this work was to evaluate a method for the estimation of the effective renal plasma flow (ERPF) using sodium 131I-o-hippurate (OIH) by means the bicompartmental analysis of the radioactive concentration of 4 plasma samples, analyzing the error obtained respecting to the multi-sample method and comparing it with those obtained with other simplified methods. We calculated the ERPF from 62 patients using the multi-sample method as standard, the method based in a single sample at 44 minutes p.i., a method based in 2 samples from the first exponential, 4 methods based in 2 samples from the second exponential, one method based in 3 samples and 5 methods based in 4 samples (2 from the first exponential and 2 from the second exponential). Each simplified method was compared to the standard method and their absolute error (AE) and standard estimation error (SEE) were calculated. The method based in the 4 samples withdrawn at 4, 10, 30 and 60 minutes p.i. was the best of them with a correlation factor with the standard equal to 0.997, an EA that ranged from -63 ml/min to 49 ml/min (mean: -4 ml/min) and a SEE of 19.5 ml/min.

Body Fluid Compartments↗
PubMed1996

MRI in radiation-induced myelopathy and pharyngocutaneous fistula.

A patient developed a cervical myelopathy 20 months after radiotherapy for a carcinoma of the larynx. MRI showed an intramedullary lesion at C7. Although radiation myelopathy was suspected, tumour recurrence could not be excluded. A radiation-induced pharyngocutaneous fistula, confirmed histologically, appeared a month later. The fistula lay just anterior to the level of the spinal cord lesion, a finding useful in supporting a diagnosis of simultaneous radiation myelopathy.

Aged↗
PubMed1996

Cough, exertional, and sexual headaches: an analysis of 72 benign and symptomatic cases.

We analyzed our experience with cough, exertional, and vascular sexual headaches, evaluated the interrelationships among them, and examined the possible symptomatic cases. Seventy-two patients consulted us because of headaches precipitated by coughing (n = 30), physical exercise (n = 28), or sexual excitement (n = 14). Thirty (42%) were symptomatic. The 17 cases of symptomatic cough headache were secondary to Chiari type I malformation, while the majority of cases of symptomatic exertional headaches and the only case of symptomatic sexual headache were secondary to subarachnoid hemorrhage. Although the precipitant was the same, benign and symptomatic headaches differed in several clinical aspects, such as age at onset, associated clinical manifestations, or response to pharmacologic treatment. Although sharing some properties, such as male predominance, benign cough headache and benign exertional headache are clinically separate conditions. Benign cough headache began significantly later, 43 years on average, than benign exertional headache. By contrast, our findings suggest that there is a close relationship between benign exertional headache and benign vascular sexual headache. We conclude that benign and symptomatic cough headaches are different from both benign and symptomatic exertional and sexual headaches.

Adolescent↗
PubMed1989

Conformations in crystals and solutions of d(CACGTG), d(CCGCGG) and d(GGCGCC) studied by vibrational spectroscopy.

Crystals of self complementary DNA hexamers d(CACGTG), d(CCGCGG) and d(GGCGCC) were grown by vapour diffusion technique and studied by microRaman and microIR spectroscopies. The oligonucleotides were studied in parallel in solution by vibrational spectroscopy. A B- greater than Z transition was detected by Raman spectroscopy during the crystallization process for d(CACGTG). Vibrational spectroscopy shows that the d(GGCGCC) crystals adopt a B geometry. On the contrary the d(CCGCGG) sequence which is shown to be able to undergo in solution or in films quite easily the B- greater than Z transition, remains trapped in crystals in a geometry which may correspond to an intermediate conformation often proposed in models of the B- greater than Z transition. The crystals used in this study were characterized by X-ray diffraction. The unit cell and space group have been determined.

Base Sequence↗
PubMed1980

[Not Available].

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Black or African American↗