Biomedical subjects

F J Cabañes

Publications and source records attributed to F J Cabañes.

At least 37 records · Page 2Linked to original sources

PubMed2000

Pneumocystis carinii pneumonia in a Yorkshire terrier dog.

A 14-month-old male Yorkshire terrier was presented to the Autonomous University of Barcelona Veterinary Teaching Hospital because of a history of chronic non-productive cough and acute dyspnea. A follow-up radiograph revealed a diffuse, bilaterally interstitial-alveolar lung disease with presence of air bronchograms. The dog died 5 h after admission with severe dyspnea. Histological sections of the necropsy specimens revealed the presence of characteristic Pneumocystis carinii cysts within alveolar spaces. A diagnosis of P. carinii pneumonia (PCP) was made on the basis of these results. To our knowledge, PCP has not been described in a Yorkshire terrier dog.

Animals↗

PubMed2000

Evaluation of different preservation and storage methods for Malassezia spp.

Freezing at -80 degrees C, lyophilization, preservation in distilled water, and storage in different culture media were performed in order to find a suitable method that allowed a prolonged storage of Malassezia spp. Freezing at -80 degrees C was the only method successful at maintaining all species.

Animals↗

PubMed2000

Otitis externa associated with Malassezia sympodialis in two cats.

The lipid-dependent species Malassezia sympodialis was isolated from two cats with otitis externa. To our knowledge, this is the first report of the isolation of lipid-dependent species of the genus Malassezia associated with skin disease in domestic animals.

Animals↗

PubMed2000

Atypical lipid-dependent Malassezia species isolated from dogs with otitis externa.

During a survey of the occurrence of Malassezia species in the external ear canals of dogs with chronic otitis externa, lipid-dependent Malassezia species were isolated in three dogs. These species were identified as Malassezia furfur and M. obtusa but showed atypical assimilation patterns. To our knowledge, this is the first report of the isolation of lipid-dependent species of the genus Malassezia in association with canine otitis.

Animals↗

PubMed2000

Prominent animal mycoses from various regions of the world.

The mycoses selected for presentation in this section are relatively common diseases of companion animals or livestock in certain areas of the world. Malasseziosis is arguably the most frequent mycosis of dogs (as otitis externa and dermatitis) throughout the world, although its diagnosis is often overlooked. Protothecosis is also geographically widespread, particularly in cattle where severe mastitis is a result of adventitious infection from the environment. In contrast, coccidioidomycosis and pythiosis are geographically limited in their occurrence (coccidioidomycosis by geographic region and pythiosis by climate), but within regions where they do occur, their presence in animals is not unusual. It was our intention to review recent developments in each of these diseases.

Animals↗

PubMed2000

[Animal dermatophytosis. Recent advances].

The proportion of positive samples in relation to the number of samples examined from cases of dog and cat dermatophytosis varies considerably from one investigation to another. In dogs, it ranges between 4% and 10% and few studies show higher prevalences. On the other hand, the percentages of positive cultures cited in the reviewed literature from dogs with or without suspected dermatophytosis are quite similar. In dogs with suspected lesions of dermatophytosis, with few exceptions, Microsporum canis is the most common species isolated. Trichophyton mentagrophytes and Microsporum gypseum are less frequently isolated. In cats the prevalence of dermatophytes is usually higher than in dogs, and it is usually higher than 20%. However the frequency of positive findings is higher in cats with suspected dermatophytosis than in cats without visible lesions, with the exception of asymptomatic infected cats and transient carrier cats. Cats are accepted as the principal reservoir for M. canis. Griseofulvin is the drug of choice in canine and feline dermatophytosis.

English Abstract↗

PubMed2000

[Aspergillus and nosocomial aspergillosis].

The current importance of nosocomial aspergillosis has prompted this symposium to review the most important issues in the field: taxonomy and identification of species involved, clinical presentations, diagnosis, treatment, prevention and molecular epidemiology applied to the detection of outbreaks of nosocomial aspergillosis.

English Abstract↗

PubMed2000

[Emerging mycotoxins: introduction].

Mycotoxins are a diverse group of secondary metabolites produced by moulds. They have a wide range of toxicological effects both in humans and animals. Nowadays about 200 of these metabolites have been described, but only a few of them may be considered as important from the agricultural point of view. In this publication, some aspects such as mycotoxin producing species, analytical methods, natural occurrence, preventive and detoxification techniques and regulations of these kind of mycotoxins will be developed by different authors involved in these research issues.

English Abstract↗

PubMed2000

[Mycotoxin producing fungi].

Mycotoxins are relatively small molecules characterized by a diversity of chemical structure and a diversity of biological activity. They are often genotypically specific for a group of species, but the same compound can also be formed by fungi belonging to different genera. Most of the mycotoxins known have been recognized as metabolic products of Aspergillus, Penicillium and Fusarium species. This review will be focused on aflatoxins, ochratoxins and fumonisins because of their hazard to animal and human health. The production of these mycotoxins have been usually associated with a small number of species but some recent studies have reported the production of these mycotoxins by some other species. These results show that mycotoxin production is broader than is normally thought, so the possibility can not be ruled out that new species may be a new source of unexpected mycotoxins in their natural substrates.

English Abstract↗

PubMed1999

New PCR method to differentiate species in the Aspergillus niger aggregate.

The DNA that encodes the 5.8S gene of the ribosomal RNA and the two intergenic spacers ITS1 and ITS2 of the two proposed type strains of the Aspergillus niger aggregate (A. niger and Aspergillus tubingensis) have been sequenced. By comparison of sequences we have found that both species could be differentiated by RsaI digestion of the PCR products of the mentioned regions. This method could be a useful tool in the identification of strains of the A. niger aggregate, especially in studies that involve a large number of isolates.

Aspergillus niger↗

PubMed1999

Surveillance of fumonisins in maize-based feeds and cereals from spain.

A survey has been carried out to determine the levels of fumonisins in 171 samples of maize-based feeds and cereals available in Spain. Also, the samples were examined for mold count and fungal species. Aspergillus, Penicillium, and Fusarium were the most frequent genera, and Fusarium and Aphanocladium had the highest individual percentage counts. Regarding Fusarium species, F. moniliforme (47. 4%) was the predominant species; F. proliferatum (5.3%) and F. subglutinans (7.0%) were isolated at low frequency. The high-performance liquid chromatography-o-phthaldialdehyde fluorescence method was used for the analysis of fumonisins. The highest levels of fumonisins were detected in maize. Overall, fumonisin B(1) (FB(1)) and fumonisin B(2) (FB(2)) were detected in 79.5 and 14.6%, of samples respectively, with average FB(1) levels of 3.3 microg/g and average FB(2) levels of 1.7 microg/g. Low levels of fumonisins in wheat, barley, and soybean were detected. This would appear to be the first report of concentrations of fumonisins in these commodities.

Animal Feed↗

PubMed1999

Isolation of Malassezia furfur from a cat.

During a survey of the occurrence of Malassezia species in the external ear canals of cats without otitis externa, Malassezia furfur was isolated. This is the first report of the isolation of M. furfur from cats.

Animals↗

PubMed1999

Fumonisin production by Fusarium species isolated from cereals and feeds in Spain.

The ability of Fusarium species to produce fumonisins was studied with 145 isolates of the following species: F. moniliforme (119 isolates), F. subglutinans (12 isolates), F. proliferatum (9 isolates), F. avenaceum (1 isolate), F. oxysporum (1 isolate), and F. semitectum (3 isolates). All isolates were cultured on autoclaved corn kernels. The production of fumonisins was determined by a high-performance liquid chromatography-o-phthaldialdehyde-fluorescence method. Fumonisin production was restricted to isolates of F. moniliforme (94.1%) and F. proliferatum (100%), in the section Liseola, including all strains isolated from wheat, barley, peas, and soybean. One strain of F. proliferatum isolated from maize produced 30,949 microg/g of fumonisin B1 and 16,966 microg/g of fumonisin B2.

Animal Feed↗

PubMed1999

DNA fingerprinting of Fusarium solani isolates related to a cutaneous infection in a sea turtle.

Random amplified polymorphic DNA (RAPD) fingerprinting was applied to Fusarium solani from a cutaneous hyalohyphomycosis in a loggerhead sea turtle. A total of seven F. solani isolates were examined, three from culture collections and four from the turtle infection (one from the turtle's lesions and three from the sand of the tank in which the turtle was kept). The banding patterns of the isolates from culture collections were markedly different from the patterns of the isolates from both the turtle and the sand. The RAPD banding patterns were the same with all the primers used, suggesting that this opportunistic infection may be related to the presence of F. solani in the tank. RAPD techniques can be particularly useful for large-scale epidemiological studies and for identifying sources of infection.

Animals↗

PubMed1998

Nasal granuloma caused by Scedosporium apiospermum in a dog.

A 10-month-old male American Staffordshire terrier was presented to the Autonomous University of Barcelona Veterinary Teaching Hospital because of a 6-month history of a mucopurulent bilateral nasal discharge. The dog had not responded to antibiotics. A follow-up X ray revealed a mixed pattern of osteolysis and increased radiodensity confined to the nasal cavity. Histologic sections of the biopsy specimens revealed the presence of granules containing numerous septate hyphae that were hyaline to pale brown and smooth, one-celled, subspherical-to-elongate conidia that were hyaline to brownish green, and bacteria. Cultures yielded numerous colonies belonging to Scedosporium apiospermum. Susceptibility tests were performed on the isolated strain. The isolate was sensitive to ketoconazole, intermediate to clotrimazole, and resistant to amphotericin B, 5-fluorocytosine, fluconazole, and itraconazole. The dog was treated with oral ketoconazole. During the treatment a general improvement in the lesions was observed. To our knowledge, S. apiospermum has not been implicated previously as an etiologic agent of nasal disease in dogs. This report provides its first description as such.

Animals↗

PubMed1997

Dermatophytes isolated from domestic animals in Barcelona, Spain.

This retrospective study deals with the main samples studied at the Mycology Diagnostic Service of the Faculty of Veterinary Science of Barcelona: animals with suspected dermatophytosis. Over a ten-year period from 1986 to 1995, 136 dermatophytes were identified from dog and cat cultures submitted for identification and from specimens submitted for mycological examination from a variety of other domestic animals. The most frequent dermatophytes isolated were Microsporum canis (55.9%), Trichophyton mentagrophytes var. mentagrophytes (27.2%), Microsporum gypseum (7.4%) and Trichophyton verrucosum (7.4%). The identity of the dermatophytes from dog and cat cultures submitted for identification was M. canis (77.8%), T. mentagrophytes (13.3%) and M. gypseum (8.9%). Dermatophytes were cultured from 15 of 105 (14.3%) canine specimens and 19 of 56 (33.9%) feline specimens submitted for mycological examination during this period. Microsporum canis was the most common species isolated (73.3% and 94.7% respectively). The percentage of positive microscopic examinations of the specimens of hair in culture positive submissions from dogs and cats was 58.8%. There was a high proportion of positive cultures from both dogs and cats less than 1 year of age, and in some breeds of dogs, but there was no significant difference between the sexes. Although dermatophytes were more frequently isolated in autumn and winter months, no significant difference was detected in the seasonal distribution of the canine and feline dermatophytosis. Trichophyton mentagrophytes was the most prevalent dermatophyte in rabbits and T. verrucosum in ruminants. Other isolated species were T. equinum and M. equinum from horses.

Animals↗

PubMed1997

Malachite green agar, a new selective medium for Fusarium spp.

Malachite Green Agar 2.5 ppm (MGA 2.5) is a potent selective medium for isolation and enumeration of Fusarium spp. It has been tested with pure and mixed cultures as well as in naturally contaminated samples. The recoveries of Fusarium species in MGA 2.5 were the same as the recoveries obtained in Nash and Snyder medium. However, this medium is a more selective culture medium for Fusarium spp. than Nash and Snyder medium, because it does not allow the development of colonies belonging to other fungal genera. MGA 2.5 is simple to prepare and less hazardous than other Fusarium selective media containing pentachloronitrobenzene (PCNB).

Journal Article↗

PubMed1997

Cutaneous hyalohyphomycosis caused by Fusarium solani in a loggerhead sea turtle (Caretta caretta L.).

Fusarium solani was reported as the agent of a cutaneous infection in an injured sea turtle collected in the Mediterranean Sea. The turtle was treated with both a topical 10% solution of iodine in alcohol and ketoconazole. The source of the causal agent was traced to the sand in the tank in which the turtle was maintained. The strain was only sensitive in vitro to amphotericin B and was resistant to 5-fluorocytosine, fluconazole, itraconazole, and ketoconazole.

Administration, Topical↗