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Biomedical subjects

F Joachim

Publications and source records attributed to F Joachim.

18 recordsLinked to original sources
PubMed1990

A case report of chronic neutropenia: clinical and ultrastructural findings.

This case report of severe periodontitis concerns a young male patient with chronic idiopathic neutropenia seen between 1981 and 1983 by the School of Dental Medicine of Geneva and from 1984 to 1988 by the Eastman Dental Hospital of London. The patient was maintained by intermittent systemic antibiotics, subgingival chlorhexidine irrigation, conventional debridement, brushing, and wire-mesh and composite splinting of loose teeth. After leaving school, at the patient's express wish, the extreme downhill pattern of the periodontitis resulted in removal of affected teeth and overdenture construction. The extracted teeth and associated soft tissues were examined for relevant plaque and host response features. The intact morphotypes associated with the advancing front of the lesion were invariably Gram-negative or positive coccoid cells, or less commonly short rods, as in all documented forms of periodotitis. Connective tissue destruction was associated with a leucocyte accumulation comprising mainly polymorphonuclear neutrophils (PMN) and plasma cells. Both were observed degenerated more deeply than in routine adult periodontitis, including PMN lysosome loss and lymphocyte maturation within peripheral blood vessels. Only in one instance, one part of the superficial connective tissue of one block contained bacteria. It was concluded that the features of plaque and the lesion suggest a typical first line of defence response as in other forms of periodontitis. From the consideration of the 10 years clinical history of the patient, it is clear that non-surgical management is possible even in extreme cases, without any compromise of the patient's oral or systemic health, and in accord with the patient's wishes.

Adolescent↗
PubMed1990

The plasma cell at the advancing front of the lesion in chronic periodontitis.

This study analyses the ultrastructure of the plasma cell population of periodontitis-affected soft tissue close to the advancing front of interdental lesions. Biopsies from 20 patients and 3 control volunteers were examined: 5 with treated adult periodontitis (AP), 5 with untreated AP, 5 with treated juvenile or post-juvenile periodontitis (JP) and 5 with untreated JP. Plasma cell (PC) counts increased significantly (p less than 0.05) with lesion severity. They were absent from epithelium and sparse in the clinically healthy control specimens. Degenerate PC tended to be more numerous within JP tissue but differences were not significant (p greater than 0.05) when compared to AP. Intact plasma cells were never seen within JP superficial connective tissue. Russell bodies were small and few in number. The presence of degenerated plasma cells indicated normal formation and release of immunoglobulins within the tissues of AP and JP. Increased counts of degenerate PC and tissue destruction in JP suggested a correlation, possibly attributable to anti-collagen antibody secretion.

Adolescent↗
PubMed1990

Interleukins and their relevance to the pathogenesis of periodontal disease.

The host response to exogenous antigen involves a complex series of interactions amongst macrophages, lymphocytes and other cells of the inflammatory system. Many, if not all, of these interactions are mediated by small molecular weight polypeptides known as cytokines. These key mediators of the host response include the interleukins, tumor necrosis factors and interferons. The biological properties of interleukin-1, -2, -4 and -6 are discussed and proposals made regarding their possible role in the pathogenesis of periodontal diseases.

Alveolar Bone Loss↗
PubMed1989

[Air polishing instruments. Review of literature].

The popularity of polishing air devices (AP) is rising with the increased distribution of commercial units in both United States and European dental offices and clinics. The instrument's efficiency and effectiveness in stain removal has been demonstrated with minimal impact on soft tissue trauma and abrasion. This article reviews the literature on AP. Future research is indicated to explore surgical clinical applications of airpowder polishing as well as more detailed information regarding the biological basis for its use.

Air↗
PubMed1989

[Aspects of distribution of plasma cells at the advancing front of the lesion in chronic periodontitis: a quantitative ultrastructural study].

The aim of this study was to compare the distribution of plasma cells within the soft tissue walls of interdental deep pockets from patients with adult (AP) and juvenile/post-juvenile (JP/PJP) periodontitis. Biopsies from 20 patients and 3 control volunteers were examined: 5 with treated AP, 5 with untreated AP, 5 with treated JP/PJP and 5 with untreated JP/PJP. No plasma cells were seen within the epithelium from any of the biopsies examined, and they were very sparse within the connective tissue from the control specimens. In all the pathological specimens examined there was marked tissue destruction. The percentage density of plasma cells tended to be statistically significantly higher in JP/PJP than in AP, and in untreated than in treated lesions. The statistical analysis showed also that plasma cells were more abundant in a layer deep to the neutrophils lining the basement membrane. Plasma cells were also more abundant in areas of extensive histological inflammation. The plasma cells were often degenerate and such degeneration appeared more evident in JP/PJP and in areas of connective tissue devoid of vascular tissue. These findings appear to indicate an association between increase of collagen destruction and increase in plasma cell percentage density. This may relate in turn to an increase in penetration and/or virulence of components of the subgingival plaque following an increase in tissue permeability. The scarcity of Russell bodies within the plasma cells would suggest that immunoglobulin production and secretion are normal.

Adult↗
PubMed1987

Effect of dental plaque on the oxidative metabolism of normal neutrophils.

The purpose of this study was to investigate the interaction between dental plaque and the oxidative metabolism of blood (PB), crevicular (CR) and salivary (SAL) PMN. Data indicated that supragingival plaque induced an in vitro production of chemiluminescence by both PB and CR--PMN in a dose-dependent manner with a maximum activity after 30 min incubation. Comparison between PB, CR and SAL-PMNs indicated that 1) both CR and SAL-PMNs spontaneously produced large quantities of oxygen radicals, 2) CR and SAL-PMNs further produced oxygen radicals upon phorbol myristate acetate or opsonized-zymosan stimulation, and 3) SAL-PMN could not be further activated by supragingival plaque.

Dental Plaque↗
PubMed1985

Mutagenicity of azo dyes in the Salmonella/microsome assay using in vitro and in vivo activation.

The mutagenicity of 6 azo dyes, including direct black 38 (DB38), direct black 19 (DB19), direct brown 95 (DB95), solvent yellow 3 (SY3), trypan blue (TPB), and food black 2 (FB2), was examined in the Salmonella/microsome assay. The effect of chemical azo reduction (dithionite) and in vivo metabolism on the mutagenicity of the dyes was also studied. In vivo azo-dye metabolites were isolated from the urine of rats intubated with dyes by XAD-2 column chromatography. Urinary metabolites from all the treated animals, except animals treated with FB2, induced frame-shift mutations in strains TA1538 and TA98 in the presence of liver S9 activation. The control urine did not increase the incidence of revertants in strains TA1538 and TA98. Thus, XAD-2 chromatography can be used to isolate genotoxic metabolites from the urine of animals intubated with azo dyes.

Animals↗
PubMed1984

Induction of unscheduled DNA synthesis in primary rat hepatocytes by benzidine-congener-derived azo dyes in the in vitro and in vivo/in vitro assays.

The genotoxicity of the benzidine-congener-derived azo dyes. Direct Blue 1 ( DB1 ), Direct Blue 14 ( DB14 ), Direct Brown 95 ( DB95 ), and Direct Red 46 ( DR46 ) was studied in the in vitro and in vivo/in vitro unscheduled DNA synthesis (UDS) assays in primary rat hepatocytes to determine if in vivo metabolism of these compounds was required for induction of UDS. Hepatocytes were isolated, cultured, and treated with the azo dyes and [3H]thymidine (in vitro assay); alternatively, in the in vivo/in vitro assay, rats were intubated with the azo dyes, the hepatocytes isolated at 17 h after dosing and incubated in a medium containing [3H]thymidine. UDS was quantified by an autoradiographic method. None of the azo dyes induced UDS in the in vitro assay. However, DR46 did induce marginal, but significant UDS in 1 experiment (1.2 net grains at 500 micrograms/ml media). No significant UDS was observed when DR46 was tested in a subsequent in vitro assay. In the in vivo/in vitro assay, DB95 (100 mg/kg), DB14 (125 mg/kg), and DR46 (100 mg/kg) induced significant UDS (12, 2.1, and 3.5 net grains, respectively). None of the azo dyes tested was mutagenic in the Salmonella/microsome assay in the presence and absence of rat liver enzymes. Therefore, in vivo reduction of azo dyes, presumably by the gut microflora, is a requirement for the genotoxicity of these azo dyes in the primary rat hepatocyte UDS assay.

Animals↗