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F K Klein

Publications and source records attributed to F K Klein.

5 recordsLinked to original sources
PubMed1984

Evaluation of accumulation of calculus in tube-fed mentally handicapped patients.

A group of tube-fed, nonambulatory, and multihandicapped clients and a similar nontube-fed group were compared to determine the rate of calculus formation over 90 days. The findings indicate that calculus formation is significantly more rapid in the tube-fed group: the majority (71%) of this calculus was formed within the first 30 days; there was no significant difference in plaque formation between the two groups, and the oral hygiene level for both groups was poor. Further study is in progress to determine the impact of rapid buildup of calculus on oral health; any disadvantage caused the patient by frequent prophylaxis; and possible causes of the rapid onset of calculus formation.

Adolescent↗
PubMed1980

Determination of pyrimidine ribotide and deoxyribotide pools in cultured cells and mouse liver by high-performance liquid chromatography.

High-performance liquid chromatographic (HPLC) assay for determining tissue pools of uridine, deoxyuridine, cytidine, deoxycytidine, and thymidine mono-, di-, and triphospates is presented. The method utilizes anion-exchange and, after conversion of nucleotides to nucleosides by acid phosphatase, reversed-phase chromatography on a preparative column with UV detection at 254 and 280 nm. The yield of this procedure is 80 +/- 2% with a sensitivity limit of 100 pmole nucleotide per sample. A sensitivity of 10 pmole can be achieved for each compound by rechromatographing appropriate nucleoside fractions on analytical columns. The recovery, including this step, is 66 +/- 7%. The assay is reproducible and highly selective, with a lower sensitivity limit of approximately 0.1 muM using 150--250 mg (wet weight) tissue samples. Nucleotide pools have been determined in Balb/c mouse liver and in mouse lymphoma (S-49) cell culture, the latter with and without addition of 5-fluorouracil (5-FUra) to the medium. Data obtained with this assay are similar to those using alternative methodologies. Observed depletion of dTXP pools and expansion of dUMP and dCXP pools after 5-FUra treatment are in agreement with published observations. Pools of dUDP and dUTP were not detectable (less than 10 pmole/10(8) cells) in any tissue sample. These data illustrate the utility of the present method in studying actions of pyrimidine antimetabolites.

Animals↗